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anti-Monkey BAX antibody for ELISA

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • bax-A
  • xBax
  • xlbax
  • BAX
  • BCL2L4
  • BAX-ALPHA
  • bcl2-associated X protein, a
  • BCL2-associated X protein
  • Bcl2-associated X protein
  • baxa
  • bax
  • BAX
  • Bax
  • LOC100355675
Reactivity
Human, Monkey
496
169
155
64
23
22
16
13
9
2
2
1
1
1
Host
Mouse
323
210
4
Clonality
Monoclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
11
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
367
215
139
125
118
112
94
49
39
34
11
6
6
4
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN3026586
$ 361.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN4283112 ELISA Alexa Fluor 405 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283113 ELISA Alexa Fluor 488 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283114 ELISA Alexa Fluor 647 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283115 ELISA Alexa Fluor 700 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283117 ELISA Biotin Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283118 ELISA DyLight 650 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283119 ELISA DyLight 680 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283120 ELISA DyLight 488 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283122 ELISA DyLight 755 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283123 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PerCP Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283125 ELISA DyLight 550 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283126 ELISA DyLight 350 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283127 ELISA DyLight 405 Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283110 ELISA Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283156 ELISA FITC Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283121 ELISA HRP Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283111 ELISA Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283116 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Allophycocyanin Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4283124 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB PE Mouse IgG1 kappa AA 3-16 Log in to see 2D2
1 ABIN4901053 ELISA WB Mouse IgG2a Log in to see 2B12-G6-H6

Similar anti-BAX Antibodies

Application / Reactivity Monkey Human
Cytometry by Time of Flight (CyTOF) 2 Antibodies 4 Antibodies
ELISA 22 Antibodies 207 Antibodies
Flow Cytometry (FACS) 35 Antibodies 92 Antibodies
Immunocytochemistry (ICC) 6 Antibodies
Immunofluorescence (IF) 17 Antibodies
Immunohistochemistry (IHC) 13 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 10 Antibodies
Western Blotting (WB) 40 Antibodies
Cellular Assay (CA) 1 Antibodies
Dot Blot (DB) 1 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunoassay (IA) 1 Antibodies
Immunochromatography (IC) 6 Antibodies

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Reactivity Human, Monkey
(496), (169), (155), (64), (23), (22), (16), (13), (9), (2), (2), (1), (1), (1)
Host Mouse
(323), (210), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (14), (13), (11), (8), (7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(367), (215), (139), (125), (118), (112), (94), (49), (39), (34), (11), (6), (6), (4), (2), (2), (1), (1), (1), (1), (1), (1)
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling Images
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Characteristics This antibody recognizes a protein of 21 kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
Purification Protein G affinity chromatography
Immunogen Amino acids GSGEQPRGGGPTSS (3-16) were used as the immunogen for this anti-human Bax antibody.
Clone 2D2
Isotype IgG1

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background This antibody recognizes a protein of 21 kDa, identified as the Bax protein. The Bax antibody is highly specific and shows no cross-reaction with Bcl-2 alpha or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and the protein also counters the death repressor activity of Bcl-2.
Gene ID 581
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling Images back to top
Application Notes The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Bax antibody to be titered up or down for optimal performance.

1. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA buffer, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. ELISA: order BSA-free format for coating,FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,WB: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details Images back to top
Concentration 0.2 mg/mL
Buffer 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment Store the Bax antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BAX antibody (BCL2-Associated X Protein) (ABIN3026586) IHC testing of Hodgkin's lymphoma stained with Bax antibody (Clone 2D2).