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anti-Mouse (Murine) BAX antibody for Western Blotting

Recommended BAX Antibody (supplied by: Log in to see )

Antigen
BCL2-Associated X Protein (BAX) Antibodies
  • bax
  • fj16e01
  • wu:fc50b10
  • wu:fj16e01
  • bax-A
  • xBax
  • xlbax
  • BAX
  • BCL2L4
  • BAX-ALPHA
  • bcl2-associated X protein, a
  • BCL2-associated X protein
  • Bcl2-associated X protein
  • baxa
  • bax
  • BAX
  • Bax
  • LOC100355675
Epitope
AA 3-16, N-Term
116
61
30
27
18
15
15
13
13
13
12
12
11
9
8
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Mouse (Murine)
497
168
155
65
23
22
16
13
9
2
2
1
1
1
Host
Mouse
323
210
4
Clonality
Monoclonal
Conjugate
This BAX antibody is un-conjugated
19
14
13
11
8
7
7
6
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
367
216
140
125
118
112
95
49
39
34
11
6
5
4
2
2
1
1
1
1
1
1
Supplier
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Catalog No. ABIN492362
$ 346.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590793 ABIN222887 IP WB Rabbit IgG N-Term Log in to see Polyclonal 5
2.9590793 ABIN1534426 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 2
2.9590793 ABIN1532504 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
2.9590793 ABIN451743 ELISA FACS ICC IF IHC IHC (fro) IP WB Mouse IgG1 kappa N-Term Log in to see 6A7 2
2.9590793 ABIN967537 IP WB Mouse IgG1, kappa N-Term Log in to see 6A7 6
2.9590793 ABIN682843 IF (p) IHC (p) WB Rabbit IgG AA 179-192, pSer184 Log in to see Polyclonal 5
2.9590793 ABIN725390 FACS IF (cc) IF (p) IHC (p) WB Rabbit IgG AA 60-110 Log in to see Polyclonal 20
2.9590793 ABIN4283098 IHC IHC (fro) IHC (p) IP WB Rabbit Log in to see Polyclonal 2
2.9590793 ABIN1105496 WB Rabbit IgG N-Term Log in to see Polyclonal
2.9590793 ABIN4283102 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 2
2.9590793 ABIN3183486 ELISA IF WB Rabbit IgG Ser165 Log in to see Polyclonal
2.9590793 ABIN3183484 ELISA IF IHC WB Rabbit IgG Internal Region Log in to see Polyclonal
2.9590793 ABIN3183487 ELISA IHC WB Rabbit IgG N-Term Log in to see Polyclonal
2.9590793 ABIN2705573 IC IF IHC WB Rabbit Center Log in to see Polyclonal
2.9590793 ABIN2707357 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN2957872 IC IF WB Rabbit C-Term Log in to see Polyclonal
2.9590793 ABIN498950 IF WB Rabbit Log in to see Polyclonal
2.9590793 ABIN155065 WB Mouse IgG2a Log in to see 6036A7
2.9590793 ABIN2707358 IHC WB Rabbit N-Term Log in to see Polyclonal
2.9590793 ABIN1871227 IHC WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-BAX antibody for Western Blotting

Similar anti-BAX Antibodies

Application / Reactivity Mouse (Murine)
ELISA 58 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Flow Cytometry (FACS) 23 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 10 Antibodies
Immunofluorescence (IF) 39 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 61 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 6 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 25 Antibodies
Immunoprecipitation (IP) 47 Antibodies
Western Blotting (WB) 107 Antibodies

General

Antigen BCL2-Associated X Protein (BAX) Antibodies
Epitope AA 3-16, N-Term
(116), (61), (30), (27), (18), (15), (15), (13), (13), (13), (12), (12), (11), (9), (8), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Mouse (Murine)
(497), (168), (155), (65), (23), (22), (16), (13), (9), (2), (2), (1), (1), (1)
Host Mouse
(323), (210), (4)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BAX antibody is un-conjugated
(19), (14), (13), (11), (8), (7), (7), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(367), (216), (140), (125), (118), (112), (95), (49), (39), (34), (11), (6), (5), (4), (2), (2), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-BAX Antibody

Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN492362) Images
Specificity This antibody reacts with Mouse Bax (21 kDa) on Western blotting.
Cross-Reactivity (Details) Species reactivity (tested):Mouse.
Characteristics Synonyms: Apoptosis regulator BAX, BCL2L4, Bcl2-L-4, Bcl-2-like protein 4, BCL2-associated X protein
Purification Protein-A Sepharose Chromatography of hybridoma supernatant.
Immunogen Synthetic peptide (KLH conjugated) corresponding to amino acids 3–16 near theN-terminus of Mouse Bax. AA Sequence: GSGEQLGSGGPTSSRemarks: The immunogen sequence is not present in Human or Rat Bax. Hybridoma was established by fusion of mouse myelomacell NS-1 with Balb/c mouse splenocyte.
Clone 5B7
Isotype IgG1

Target Details BAX

Product Details anti-BAX Antibody Application Details Handling References for anti-BAX Antibody (ABIN492362) Images back to top
Antigen
Alternative Name Bax (BAX Antibody Abstract)
Background Bax (Bcl-associated X protein) is a 21 kDa tumor suppressor protein that suppresses tumorigenesis and stimulates apoptosis in vivo. Bax has extensive amino acid homology to Bcl-2. It can homodimerize through its BH3 domain and it forms heterodimers with other Bcl-2 family members through its BH1 and BH2 domains. Overexpression of Bax promotes apoptosis and counters the death repressor activity of Bcl-2 and Bcl-xL. It is believed that the ratio of Bcl-2/Bax complexes to free protein controls the relative susceptibility of cells to death stimuli. Apoptotic stimuli cause the translocation of monomeric Bax from the cytosol to the mitochondria where it forms Bax homodimers. Localization of Bax to the mitochondria results in the activation of caspase-3, membrane blebbing, and nuclear fragmentation. Bax also induces mitochondrial dysfunction by increasing mitochondrial membrane permeability. It accelerates the opening of the mitochondrial porin channel VDAC, thus regulating the release of cytochrome c during apoptosis.Synonyms: Apoptosis regulator BAX, BCL2-associated X protein, BCL2L4, Bcl-2-like protein 4, Bcl2-L-4
Gene ID 12028
UniProt Q07813
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis

Application Details

Product Details anti-BAX Antibody Target Details BAX Handling References for anti-BAX Antibody (ABIN492362) Images back to top
Application Notes ELISA. Western blotting: 1 μg/mL for chemiluminescence detection system. Immunofluorescence. Immunoprecipitation: 2 μg/200 μL of cell extract from 5x10^6 cells. Detailed procedure is provided in Protocols.
Protocol SDS-PAGE & Western Blotting1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl, pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10%glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. Measure the protein concentration of the supernatant and add the cold Lysisbuffer to make 8 mg/mL solution. 3) Mix the sample with equal volume of Laemmli's sample buffer. 4) Boil the samples for 3 minutes and centrifuge. Load 10 μL of the sample per lane in a 1mm thick SDS-polyacrylamide gel for electrophoresis. 5) Blot the protein to a polyvinylidene difluoride (PVDF) membrane at 1 mA/cm2 for 1 hourin a semi-dry transfer system (Transfer Buffer: 25 mM Tris, 190 mM glycine, 20% MeOH). See the manufacture's manual for precise transfer procedure. 6) To reduce nonspecific binding, soak the membrane in 10% skimmed milk (in PBS, pH7. 2) for 1 hour at room temperature, or overnight at 4°C. 7) Incubate the membrane with primary antibody diluted with PBS, pH 7. 2 containing 1%skimmed milk as suggest in the APPLICATIONS for 1 hour at room temperature. (Theconcentration of antibody will depend on condition. )8) Wash the membrane with PBS-T [0. 05% Tween-20 in PBS] (5 minutes x 3 times). 9) Incubate the membrane with the 1: 10,000 POD-conjugated anti-mouse IgG diluted with1% skimmed milk (in PBS, pH 7. 2) for 1 hour at RT. 10) Wash the membrane with PBS-T (10 minutes x 3 times). 11) Wipe excess buffer on the membrane, then incubate it with appropriatechemiluminescence reagent for 1 minute. Remove extra reagent from the membrane by dabbing with paper towel, and seal it inplastic wrap. 12) Expose to an X-ray film in a dark room for 3 minutes. Develop the film as usual. The condition for exposure and development may vary. Positive Controls: NIH/3T3, BaF/3,WR19LImmunoprecipitation1) Wash the cells 3 times with PBS and suspend with 10 volume of cold Lysis buffer (50 mMTris-HCl pH 7. 2, 250 mM NaCl, 0. 1% NP-40, 2 mM EDTA, 10% glycerol) containingappropriate protease inhibitors. Incubate it at 4°C with rotating for 30 minutes, thensonicate briefly (up to 10 seconds). 2) Centrifuge the tube at 12,000 x g for 10 minutes at 4°C and transfer the supernatant toanother tube. 3) Add primary antibody as suggested in the APPLICATIONS into 200 µL of the supernatant.
Restrictions For Research Use only

Handling

Product Details anti-BAX Antibody Target Details BAX Application Details References for anti-BAX Antibody (ABIN492362) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.2 containing 50 % Glycerol without preservatives.
Preservative Without preservative
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-BAX Antibody (ABIN492362)

Product Details anti-BAX Antibody Target Details BAX Application Details Handling Images back to top
Background publications

Lowe: "Colipase stabilizes the lid domain of pancreatic triglyceride lipase." in: The Journal of biological chemistry, Vol. 272, Issue 1, pp. 9-12, 1997

Images

Product Details anti-BAX Antibody Target Details BAX Application Details Handling References for anti-BAX Antibody (ABIN492362) back to top
Supplier Images
Western Blotting (WB) image for anti-BAX antibody (BCL2-Associated X Protein) (AA 3-16) (ABIN492362) anti-BCL2-Associated X Protein (BAX) (AA 3-16), (N-Term) antibody
Western Blotting (WB) image for anti-BAX antibody (BCL2-Associated X Protein) (AA 3-16) (ABIN492362) anti-BCL2-Associated X Protein (BAX) (AA 3-16), (N-Term) antibody (Image 2)