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|Application / Reactivity||Human|
|Enzyme Immunoassay (EIA)||9 Antibodies|
|Flow Cytometry (FACS)||7 Antibodies|
|Immunochromatography (IC)||3 Antibodies|
|Immunocytochemistry (ICC)||54 Antibodies|
|Immunofluorescence (IF)||46 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies|
|Immunohistochemistry (IHC)||67 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||2 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||34 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||262 Antibodies|
|Antigen||BCL2 Binding Component 3 (BBC3) Antibodies|
|Conjugate||This BBC3 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
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Product Details anti-BBC3 AntibodyTarget Details BBC3 Application Details Handling Images
|Specificity||This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recognized on the western blot gel.|
|No Cross-Reactivity||Mouse (Murine), Rat (Rattus)|
|Immunogen||A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen.|
Target Details BBC3Product Details anti-BBC3 Antibody Application Details Handling Images back to top
|Alternative Name||PUMA (BBC3 Antibody Abstract)|
PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cellsundergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA inducescytochrome c release and activates the rapid induction of programmed cell death.PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrialtranslocation and multimerization of BAX. PUMA also plays a role in mediatingp53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Twoisoform of PUMA are known, PUMA-alpha and PUMA-beta. Alternate Names: anti-BBC 3 antibody, anti-BBC3 antibody, anti-BCL 2 binding component 3 antibody,anti-BCL2 binding component 3 antibody, anti-JFY 1 antibody, anti-JFY1 antibody,anti-p53 up regulated modulator of apoptosis antibody, anti-p53 Upregulated Modulatorof Apoptosis antibody, anti-PUMA alpha antibody, anti-PUMA/JFY1 antibody.
Gene Symbol: BBC3
|Gene ID||27113, 170770, 317673|
|Research Area||Cancer, Apoptosis/Necrosis, Metabolism|
Application DetailsProduct Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
In Western blot this antibody detects a band at approximately 18 kDa.
Recommended dilutions: Flow Cytometry 1:30, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000-10000
Protocol specific for PUMA Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
|Restrictions||For Research Use only|
HandlingProduct Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
|Buffer||PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide|
|Precaution of Use||WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage Comment||-20 °C.|
ImagesProduct Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top