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anti-Human BBC3 antibody for Immunohistochemistry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • JFY-1
  • JFY1
  • Puma
  • PUMA
  • PUMA/JFY1
Epitope
C-Term
96
53
21
15
13
13
13
11
8
4
3
3
3
2
2
1
1
1
1
Reactivity
Human
276
82
58
2
1
1
Host
Rabbit
244
28
14
Clonality
Monoclonal
Conjugate
This BBC3 antibody is un-conjugated
13
10
8
8
8
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
263
84
66
53
44
34
11
9
6
4
3
2
2
Supplier
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Available images

Catalog No. ABIN258427
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.22865479 ABIN1031530 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 1
0.22865479 ABIN252347 IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal 5
0.22865479 ABIN2159473 IHC WB Rabbit Ig Fraction Center Log in to see Polyclonal
0.22865479 ABIN2491786 FACS IF IHC WB Rabbit Ig Fraction BH3 Domain Log in to see Polyclonal
0.0011340706 ABIN361754 IHC WB Rabbit N-Term Log in to see Polyclonal 4
0.0011340706 ABIN411574 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN1928225 IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
0.0011340706 ABIN1928224 IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
0.0011340706 ABIN1928226 IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
0.0011340706 ABIN1928227 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
0.0011340706 ABIN209040 IHC WB Rabbit C-Term Log in to see Polyclonal
0.0011340706 ABIN1928204 IHC ELISA WB PE Rabbit IgG AA 108-137 Log in to see Polyclonal
0.0011340706 ABIN1928202 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG AA 108-137 Log in to see Polyclonal
0.0011340706 ABIN1928205 IHC ELISA WB FITC Rabbit IgG AA 108-137 Log in to see Polyclonal
0.0011340706 ABIN1928203 IHC ELISA WB APC Rabbit IgG AA 108-137 Log in to see Polyclonal
0.0011340706 ABIN355899 IHC WB Rabbit IgG Log in to see Polyclonal
0.0011340706 ABIN1928232 IHC WB Alkaline Phosphatase (AP) Rabbit N-Term Log in to see Polyclonal
0.0011340706 ABIN1928235 IHC WB FITC Rabbit N-Term Log in to see Polyclonal
0.0011340706 ABIN1928241 IHC WB Atto 390 Rabbit N-Term Log in to see Polyclonal
0.0011340706 ABIN1928243 IHC WB Atto 565 Rabbit N-Term Log in to see Polyclonal

Top referenced anti-BBC3 antibody for Immunohistochemistry

Similar anti-BBC3 Antibodies

Application / Reactivity Human
ELISA 79 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 7 Antibodies
Immunochromatography (IC) 3 Antibodies
Immunocytochemistry (ICC) 54 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 67 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 262 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope C-Term
(96), (53), (21), (15), (13), (13), (13), (11), (8), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human
(276), (82), (58), (2), (1), (1)
Host Rabbit
(244), (28), (14)
Clonality (Clone)
Monoclonal   ( )
Conjugate This BBC3 antibody is un-conjugated
(13), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(263), (84), (66), (53), (44), (34), (11), (9), (6), (4), (3), (2), (2)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Specificity This antibody is predicted to detect both isoforms based on sequence analysis. The isoform B is ~18 kDa and isoform A is ~ 24kDa. Isoform B is primarily recognized on the western blot gel.
No Cross-Reactivity Mouse (Murine), Rat (Rattus)
Purification IgG purified
Immunogen A synthetic peptide corresponding to residues in the C-term of human PUMA was used as immunogen.
Clone EP512Y
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background PUMA, (p53 upregulated modulator of apoptosis), is activated by p53 in cellsundergoing p53-induced apoptosis (1-2). Localized in the mitochondria PUMA inducescytochrome c release and activates the rapid induction of programmed cell death.PUMA interacts with Bcl2 (3), Bcl-XL, Bcl2-like-1 protein and promotes mitochondrialtranslocation and multimerization of BAX. PUMA also plays a role in mediatingp53-induced cell death through the cytochrome c/APAF1-dependent pathway (2). Twoisoform of PUMA are known, PUMA-alpha and PUMA-beta. Alternate Names: anti-BBC 3 antibody, anti-BBC3 antibody, anti-BCL 2 binding component 3 antibody,anti-BCL2 binding component 3 antibody, anti-JFY 1 antibody, anti-JFY1 antibody,anti-p53 up regulated modulator of apoptosis antibody, anti-p53 Upregulated Modulatorof Apoptosis antibody, anti-PUMA alpha antibody, anti-PUMA/JFY1 antibody.
Gene Symbol: BBC3
Gene ID 27113, 170770, 317673
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes In Western blot this antibody detects a band at approximately 18 kDa.
Recommended dilutions: Flow Cytometry 1:30, Immunocytochemistry/Immunofluorescence 1:250-500, Immunohistochemistry 1:10-1:2000, Immunohistochemistry-Paraffin 1:100-250, Western Blot 1:1000-10000
Protocol Protocol specific for PUMA Antibody Immunohistochemistry Protocol for Paraffin-embedded Tissues
1. Solutions and reagents1.
. Xylene1.
. Ethanol, anhydrous denatured, histological grade (100 %, 95 %, 70 %)1.
. Washing buffer:TBST washing buffer: 1XTBS/0.1 % Tween-20To prepare stock solution of 10X TBS: add 24.2 g Trizma base and 80 g sodium chloride to 1L of dH2O. Adjust pH to 7.6.Working solution. 1XTBST/0.1 % Tween-20: add 100ml 10XTBS to 900 mL dH2O. Add 1 mL Tween-20 and mix well.1.
. Distilled water (dH2O)1.
. Antigen Retrieval Solution:0.01M Sodium Citrate
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Format Liquid
Buffer PBS 49 % , Glycerol 50 % , BSA 0.05 % , Sodium Azide
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid freeze-thaw cycles
Storage -20 °C
Storage Comment -20 °C.

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN258427) Western blot analysis on K562 cell lysate using anti-PUMA (C-term) RabMAb (cat. # 110...
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN258427) Immunofluorescent staining of HeLa cells using anti-PUMA (C-term) RabMAb (cat. # 110-...
Immunohistochemistry (IHC) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN258427) Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using anti-PU...