anti-Human BBC3 antibody for Immunohistochemistry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Reactivity
Human
294
122
107
18
1
1
1
Host
Rabbit
262
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
278
85
76
59
49
31
20
13
8
7
2
1
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Catalog No. ABIN4348684
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.059725 ABIN252347 IHC (p) IHC WB Rabbit Log in to see Polyclonal 5
9.059725 ABIN1031530 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal 1
9.059725 ABIN3030112 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 119-154 Log in to see Polyclonal
9.059725 ABIN3028491 IHC ELISA WB Rabbit Ig Fraction AA 109-137 Log in to see Polyclonal
9.059725 ABIN5024197 IHC WB Atto 390 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024200 IHC WB Atto 488 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024206 IHC WB Atto 594 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024212 IHC WB Atto 655 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024221 IHC WB Alkaline Phosphatase (AP) Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024224 IHC WB APC Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024227 IHC WB Biotin Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024233 IHC WB HRP Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024239 IHC WB PerCP Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024245 IHC WB Streptavidin Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024242 IHC WB PE Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024215 IHC WB Atto 680 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024203 IHC WB Atto 565 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024209 IHC WB Atto 633 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024218 IHC WB Atto 700 Rabbit N-Term Log in to see Polyclonal
9.059725 ABIN5024230 IHC WB FITC Rabbit N-Term Log in to see Polyclonal

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Reactivity Human
(294), (122), (107), (18), (1), (1), (1)
Host Rabbit
(262), (24), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(278), (85), (76), (59), (49), (31), (20), (13), (8), (7), (2), (1)
Pubmed 4 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 antibody (ABIN4348684) Images
Purification Immunogen affinity purified
Immunogen Antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the first 50 amino acids of PUMA.

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 antibody (ABIN4348684) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Gene Symbol: BBC3
Molecular Weight Theoretical MW: 23 kDa
Gene ID 27113
NCBI Accession NP_055232
Research Area Metabolism, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 antibody (ABIN4348684) Images back to top
Application Notes Western Blot 2 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 10 μg/mL, Immunohistochemistry-Paraffin 1:10-1:500, ImmunofluorescencePUMA antibody can be used for detection of PUMA by Western blot at 2 to 4 mg/mL. K562 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. antibody can also detect PUMA by immunohistochemistry at 10 mg/mL. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 antibody (ABIN4348684) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-BBC3 antibody (ABIN4348684)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Product cited in:

Long, Schoonen, Graczyk, OPrey, Ryan: "p73 engages A2B receptor signalling to prime cancer cells to chemotherapy-induced death." in: Oncogene, 2015 (Sample species: Human). Further details: Western Blotting

Fandy, Ross, Gore, Srivastava: "Flavopiridol synergizes TRAIL cytotoxicity by downregulation of FLIPL." in: Cancer chemotherapy and pharmacology, Vol. 60, Issue 3, pp. 313-9, 2007 Further details: Western Blotting

Toth, Jeffers, Nickson, Min, Morgan, Zambetti, Erhardt: "Targeted deletion of Puma attenuates cardiomyocyte death and improves cardiac function during ischemia-reperfusion." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 1, pp. H52-60, 2006 (Sample species: Rat (Rattus)). Further details: Western Blotting

Karst, Dai, Martinka, Li: "PUMA expression is significantly reduced in human cutaneous melanomas." in: Oncogene, Vol. 24, Issue 6, pp. 1111-6, 2005 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 antibody (ABIN4348684) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Immunohistochemistry: PUMA Antibody [NBP1-76639] - Analysis of PUMA in human breast t...
Immunofluorescence (IF) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Immunofluorescence: PUMA Antibody [NBP1-76639] - Analysis of PUMA in K562 cells with ...
Immunofluorescence (IF) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Immunocytochemistry/Immunofluorescence: PUMA Antibody [NBP1-76639] - K562 cells.
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Western Blot: PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Immunohistochemistry-Paraffin: PUMA Antibody [NBP1-76639] - Human breast carcinoma wi...
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Western Blot: PUMA Antibody [NBP1-76639] - Analysis for Puma alpha (NT) using IMG-458...
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN4348684) Immunohistochemistry-Paraffin: PUMA Antibody - Human breast carcinoma with PUMA anti...