anti-Human BBC3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Reactivity
Human
290
123
107
18
1
1
1
Host
Rabbit
259
24
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
274
85
75
59
50
31
20
13
9
7
2
1
Supplier
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Catalog No. ABIN4348684
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.875275 ABIN252347 IHC (p) IHC WB Rabbit Log in to see Polyclonal 5
13.875275 ABIN4348686 IHC IHC (p) IP WB Rabbit IgG C-Term Log in to see Polyclonal
13.875275 ABIN213906 ICC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN356830 EIA FACS IF IHC (p) WB Rabbit Ig BH3 Domain, C-Term Log in to see Polyclonal 3
1 ABIN658021 IHC (p) WB Rabbit Ig AA 109-137, Center Log in to see Polyclonal 1
1 ABIN388112 FACS IF IHC (p) WB Rabbit Ig AA 119-154, BH3 Domain Log in to see Polyclonal 1
1 ABIN731528 FACS IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 2
1 ABIN5555151 EIA IF IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN5551335 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN121685 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN2477602 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal 2
1 ABIN302789 IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN959855 IHC (p) WB Rabbit IgG AA 2-16 Log in to see Polyclonal
1 ABIN208703 IHC (p) WB Rabbit IgG AA 186-199 Log in to see Polyclonal
1 ABIN1804096 IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN731537 IHC (p) WB HRP Rabbit IgG AA 120-170 Log in to see Polyclonal
1 ABIN1732233 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1822879 IHC (p) WB Rabbit IgG AA 150-193 Log in to see Polyclonal
1 ABIN320459 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN731530 IHC (p) WB Biotin Rabbit IgG AA 120-170 Log in to see Polyclonal

Top referenced anti-BBC3 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-BBC3 Antibodies

Application / Reactivity Human
ELISA 74 Antibodies
Enzyme Immunoassay (EIA) 9 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunochromatography (IC) 2 Antibodies
Immunocytochemistry (ICC) 59 Antibodies
Immunofluorescence (IF) 51 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 85 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 32 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 268 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Reactivity Human
(290), (123), (107), (18), (1), (1), (1)
Host Rabbit
(259), (24), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(274), (85), (75), (59), (50), (31), (20), (13), (9), (7), (2), (1)
Pubmed 4 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN4348684) Images
Purification Immunogen affinity purified
Immunogen Antibody was raised against a synthetic peptide corresponding to 14 amino acids near the amino terminus of human PUMA-a. The sequence is identical between a and b forms of the PUMA proteins. The immunogen is located within the first 50 amino acids of PUMA.

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN4348684) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Gene Symbol: BBC3
Molecular Weight Theoretical MW: 23 kDa
Gene ID 27113
NCBI Accession NP_055232
Research Area Metabolism, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling ProductDetails: References for anti-BBC3 antibody (ABIN4348684) Images back to top
Application Notes Western Blot 2 μg/mL, ELISA 1:100-1:2000, Immunohistochemistry 1:10-1:500, Immunocytochemistry/Immunofluorescence 10 μg/mL, Immunohistochemistry-Paraffin 1:10-1:500, ImmunofluorescencePUMA antibody can be used for detection of PUMA by Western blot at 2 to 4 mg/mL. K562 cell lysate can be used as a positive control and a band at approximately 23 kDa can be detected. antibody can also detect PUMA by immunohistochemistry at 10 mg/mL. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details ProductDetails: References for anti-BBC3 antibody (ABIN4348684) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS
Buffer contains: 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

ProductDetails: References for anti-BBC3 antibody (ABIN4348684)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Product cited in:

Long, Schoonen, Graczyk, OPrey, Ryan: "p73 engages A2B receptor signalling to prime cancer cells to chemotherapy-induced death." in: Oncogene, 2015 (Sample species: Human). Further details: Western Blotting

Fandy, Ross, Gore, Srivastava: "Flavopiridol synergizes TRAIL cytotoxicity by downregulation of FLIPL." in: Cancer chemotherapy and pharmacology, Vol. 60, Issue 3, pp. 313-9, 2007 Further details: Western Blotting

Toth, Jeffers, Nickson, Min, Morgan, Zambetti, Erhardt: "Targeted deletion of Puma attenuates cardiomyocyte death and improves cardiac function during ischemia-reperfusion." in: American journal of physiology. Heart and circulatory physiology, Vol. 291, Issue 1, pp. H52-60, 2006 (Sample species: Rat (Rattus)). Further details: Western Blotting

Karst, Dai, Martinka, Li: "PUMA expression is significantly reduced in human cutaneous melanomas." in: Oncogene, Vol. 24, Issue 6, pp. 1111-6, 2005 (Sample species: Human). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling ProductDetails: References for anti-BBC3 antibody (ABIN4348684) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN4348684) Immunohistochemistry: PUMA Antibody [NBP1-76639] - Analysis of PUMA in human breast t...
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN4348684) Immunofluorescence: PUMA Antibody [NBP1-76639] - Analysis of PUMA in K562 cells with ...
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN4348684) Immunocytochemistry/Immunofluorescence: PUMA Antibody [NBP1-76639] - K562 cells.
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN4348684) Western Blot: PUMA Antibody [NBP1-76639] - K562 cell lysate with PUMA antibody at 2 u...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN4348684) Immunohistochemistry-Paraffin: PUMA Antibody [NBP1-76639] - Human breast carcinoma wi...
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN4348684) Western Blot: PUMA Antibody [NBP1-76639] - Analysis for Puma alpha (NT) using IMG-458...