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anti-Mouse (Murine) BBC3 antibody for Immunocytochemistry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
C-Term
95
62
19
18
16
12
10
9
9
8
5
5
3
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
290
123
107
1
1
1
Host
Rabbit
259
24
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
274
86
76
60
50
32
20
13
9
7
2
1
Options
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Catalog No. ABIN361748
$ 383.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN2485956 ICC IF WB HRP Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485951 ICC IF WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485952 ICC IF WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485950 ICC IF WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485946 ICC IF WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485944 ICC IF WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485945 ICC IF WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485949 ICC IF WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485948 ICC IF WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485947 ICC IF WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485960 ICC IF WB Streptavidin Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485953 ICC IF WB APC Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485954 ICC IF WB Biotin Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485955 ICC IF WB FITC Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485957 ICC IF WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485959 ICC IF WB PE Rabbit C-Term Log in to see Polyclonal 3
1 ABIN2485958 ICC IF WB PerCP Rabbit C-Term Log in to see Polyclonal 3
1 ABIN259765 ICC IF WB Rabbit AA 1-100, Isoform 1, N-Term Log in to see Polyclonal
1 ABIN213906 ICC IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN4348685 ELISA ICC IF WB Rabbit Log in to see Polyclonal

Top referenced anti-BBC3 antibody for Immunocytochemistry

Similar anti-BBC3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 20 Antibodies 74 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 9 Antibodies
Flow Cytometry (FACS) 17 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 46 Antibodies 60 Antibodies
Immunofluorescence (IF) 34 Antibodies 51 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies 13 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 17 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 108 Antibodies
Immunochromatography (IC) 2 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope C-Term
(95), (62), (19), (18), (16), (12), (10), (9), (9), (8), (5), (5), (3), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(290), (123), (107), (1), (1), (1)
Host Rabbit
(259), (24), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(274), (86), (76), (60), (50), (32), (20), (13), (9), (7), (2), (1)
Pubmed 3 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN361748) Images
Specificity Detects ~23 kDa. Detects ~16 kDa bands sometimes, possibly corresponding to PUMAβ.
Purification Protein A Purified
Immunogen C-terminal amino acids of human PUMA

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 Antibody (ABIN361748) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 up-regulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Gene ID 27116
NCBI Accession NP_001120712
UniProt Q9BXH4
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 Antibody (ABIN361748) Images back to top
Application Notes
  • WB (1:1000)
  • ICC/IF (1:100)
  • optimal dilutions for assays should be determined by the user.
Comment

1 μg/ml of SPC-166 was sufficient for detection of PUMA in 20 μg of human K562 cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:HRP as the secondary antibody.

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 Antibody (ABIN361748) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS, 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-BBC3 Antibody (ABIN361748)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Background publications

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001

Yu, Zhang, Hwang, Kinzler, Vogelstein: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001

Han, Flemington, Houghton, Gu, Zambetti, Lutz, Zhu, Chittenden: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN361748) back to top
Supplier Images
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN361748) PUMA (CT) , Human K562 cells, and Mouse 3T3 cell lysates.
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN361748) PUMA (CT), K562 cells
 image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN361748) Human breast carcinoma