anti-Mouse (Murine) BBC3 antibody for Immunohistochemistry

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Reactivity
Human, Mouse (Murine)
294
121
107
18
1
1
1
Host
Rabbit
262
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
278
85
77
60
50
31
20
13
8
7
2
1
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Available images

Catalog No. ABIN252347
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN411574 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4965576 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1928224 IHC ELISA WB APC Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1928225 IHC ELISA WB PE Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1928226 IHC ELISA WB FITC Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN1928227 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN4348680 IHC IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2577758 ELISA IHC WB Rabbit IgG C-Term Log in to see Polyclonal
1 ABIN2880419 IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN2467757 IHC WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN355899 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN4903014 FACS ICC IF IHC WB Rabbit IgG Log in to see
1 ABIN2425609 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1738631 IHC WB Rabbit IgG Log in to see Polyclonal
1 ABIN1738632 IHC WB Rabbit IgG Log in to see Polyclonal

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Reactivity Human, Mouse (Murine)
(294), (121), (107), (18), (1), (1), (1)
Host Rabbit
(262), (24), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(278), (85), (77), (60), (50), (31), (20), (13), (8), (7), (2), (1)
Pubmed 5 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 antibody (ABIN252347) Images
Specificity The amino acid sequence used as immunogen is 100 % homologous in human (PUMA a and b) and 78 % homologous in mouse and rat. Human PUMA a is a 193 amino acid protein with a predicted molecular weight of approximately 22 kD. Human PUMA b is a 131 amino acid protein with a predicted molecular weight of approximately 15 kD.
Purification Protein G purified
Immunogen A portion of amino acids 186-199 (PLPRGHRAPEMEPN ) of human PUMA a was used as the immunogen.

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 antibody (ABIN252347) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Gene Symbol: BBC3
Gene ID 27113
Research Area Metabolism, Apoptosis/Necrosis, Cancer
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 antibody (ABIN252347) Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry 1:50-1:250, Immunohistochemistry-Paraffin 1:50-1:250Immunohistochemistry-Paraffin: See Karst et al, 2005
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 antibody (ABIN252347) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS containing 0.2 % gelatin
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-BBC3 antibody (ABIN252347)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Product cited in:

Liao, Chu, Hu, Merkin, Chen, Liu, Degenhardt, White, Ryazanov: "Paradoxical Roles of Elongation Factor-2 Kinase in Stem Cell Survival." in: The Journal of biological chemistry, Vol. 291, Issue 37, pp. 19545-57, 2016 (Sample species: Mouse (Murine)). Further details: Western Blotting

Kim, Jung, Lee, Lee, Kang, Lee, Park, Han, Lee, Ramkumar, Sung, Kim: "SIRT1 activation by resveratrol ameliorates cisplatin-induced renal injury through deacetylation of p53." in: American journal of physiology. Renal physiology, Vol. 301, Issue 2, pp. F427-35, 2011

Speidel, Helmbold, Deppert: "Dissection of transcriptional and non-transcriptional p53 activities in the response to genotoxic stress." in: Oncogene, Vol. 25, Issue 6, pp. 940-53, 2006 Further details: Western Blotting

Karst, Dai, Martinka, Li: "PUMA expression is significantly reduced in human cutaneous melanomas." in: Oncogene, Vol. 24, Issue 6, pp. 1111-6, 2005 Further details: Immunohistochemistry (Paraffin-embedded Sections)

Hershko, Ginsberg: "Up-regulation of Bcl-2 homology 3 (BH3)-only proteins by E2F1 mediates apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8627-34, 2004 (Sample species: Mouse (Murine)). Further details: Western Blotting

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 antibody (ABIN252347) back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN252347) Western blot analysis for Puma a(CT) using ABIN252347 at 1:500 against 30 ug/lane of ...
Immunohistochemistry (IHC) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN252347) PUMA expression in human dysplastic nevi (premalignant melanoma).
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN252347) Immunohistochemistry-Paraffin: PUMA Antibody [ABIN2523470] - IHC analysis of a formal...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN252347) Immunohistochemistry-Paraffin: PUMA Antibody [ABIN2523470] - IHC analysis of a formal...