anti-Mouse (Murine) BBC3 antibody for Western Blotting

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Reactivity
Human, Mouse (Murine), Rat (Rattus)
293
119
106
18
1
1
1
Host
Rabbit
260
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
276
85
77
60
49
32
20
13
8
7
2
1
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Catalog No. ABIN411574
$ 245.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.2432864 ABIN731528 FACS IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 2
3.2432864 ABIN252347 IHC (p) IHC WB Rabbit Log in to see Polyclonal 5
3.2432864 ABIN259765 ICC IF WB Rabbit AA 1-100, Isoform 1, N-Term Log in to see Polyclonal
3.2432864 ABIN3186602 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
3.2432864 ABIN2485944 ICC IF WB Atto 390 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485946 ICC IF WB Atto 565 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485949 ICC IF WB Atto 655 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485951 ICC IF WB Atto 700 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485950 ICC IF WB Atto 680 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485953 ICC IF WB APC Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485957 ICC IF WB PE-Atto 594 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485954 ICC IF WB Biotin Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485958 ICC IF WB PerCP Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485959 ICC IF WB PE Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485960 ICC IF WB Streptavidin Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485952 ICC IF WB Alkaline Phosphatase (AP) Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485945 ICC IF WB Atto 488 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485947 ICC IF WB Atto 594 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485948 ICC IF WB Atto 633 Rabbit C-Term Log in to see Polyclonal 3
3.2432864 ABIN2485955 ICC IF WB FITC Rabbit C-Term Log in to see Polyclonal 3

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(293), (119), (106), (18), (1), (1), (1)
Host Rabbit
(260), (24), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(276), (85), (77), (60), (49), (32), (20), (13), (8), (7), (2), (1)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN412890
Specificity The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 177 of human PUMA
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms: PUMA/JFY1 ,JFY-1, PUMA, BBC3, bcl-2 binding component 3
Gene ID 27113
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes Western blotting (0.5-4 μg/mL)and Immunohistochemistry (5 μg/mL). However, the optimal conditions should be determined individually. The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.
Comment

Suggested positive control:
Western Blot: Jurkat cells and Rat kidney tissue, IHC: Tonsil tissue

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) antibody (ABIN411574) anti-BCL2 Binding Component 3 (BBC3) antibody