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anti-Mouse (Murine) BBC3 antibody for Western Blotting

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • JFY-1
  • JFY1
  • Puma
  • PUMA
  • PUMA/JFY1
Epitope
C-Term
95
52
21
15
13
13
13
11
8
3
3
3
2
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine)
273
79
58
2
1
1
Host
Rabbit
241
28
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
13
10
8
8
8
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
260
84
65
52
42
33
11
8
6
4
3
2
2
Supplier
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Catalog No. ABIN500557
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13398378 ABIN223064 IF WB Rabbit IgG C-Term Log in to see Polyclonal
0.13398378 ABIN411574 IHC WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN492518 EIA WB Mouse IgG1 AA 76-170 Log in to see 2A9G5
0.13398378 ABIN259766 ICC IF WB Rabbit N-Term, AA 1-100 Log in to see Polyclonal
0.13398378 ABIN252347 IHC (p) IHC WB Rabbit IgG AA 186-199 Log in to see Polyclonal
0.13398378 ABIN1871229 WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1030609 ICC IF ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13398378 ABIN3186602 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.13398378 ABIN2498246 ICC ELISA WB Rabbit C-Term Log in to see Polyclonal
0.13398378 ABIN2425609 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN2431101 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13398378 ABIN1031786 ELISA WB Mouse Log in to see 2A9G5
0.13398378 ABIN2498247 ELISA WB Mouse Log in to see 2A9G5
0.0011731827 ABIN356830 EIA FACS IF IHC (p) WB Rabbit Ig BH3 Domain, C-Term Log in to see Polyclonal 3
0.0011731827 ABIN401380 EIA IF WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.0011731827 ABIN731528 IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 1
0.0011731827 ABIN361748 ICC IF WB Rabbit C-Term Log in to see Polyclonal 3
0.0011731827 ABIN264891 IF WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.0011731827 ABIN264892 IF WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.0011731827 ABIN121685 IHC (p) WB Rabbit IgG N-Term Log in to see Polyclonal

Top referenced anti-BBC3 antibody for Western Blotting

Similar anti-BBC3 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 15 Antibodies 79 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 9 Antibodies
Flow Cytometry (FACS) 1 Antibodies 7 Antibodies
Immunocytochemistry (ICC) 26 Antibodies 54 Antibodies
Immunofluorescence (IF) 30 Antibodies 46 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies
Immunohistochemistry (IHC) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 70 Antibodies
Immunochromatography (IC) 3 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope C-Term
(95), (52), (21), (15), (13), (13), (13), (11), (8), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(273), (79), (58), (2), (1), (1)
Host Rabbit
(241), (28), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(13), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(260), (84), (65), (52), (42), (33), (11), (8), (6), (4), (3), (2), (2)
Pubmed 3 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN500557) Images
Specificity This antibody detects PUMA at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Peptide Affinity Chromatography
Immunogen Synthetic peptide corresponding to 14 amino acids near the carboxy terminus of Human PUMA alpha. .Remarks: The sequence is identical between alpha and beta forms of the PUMA proteins.
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 Antibody (ABIN500557) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Molecular Weight 23 kDa
Gene ID 27113
NCBI Accession NP_055232
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 Antibody (ABIN500557) Images back to top
Application Notes ELISA. Western blot: 1 - 2 μg/mL. K562 or 3T3 cell lysate can be used as a positive control and aband at approximately 23 kDa can be detected. A lower band at approximately 16 kDa wasdetected in MOLT4 and U937 cells, which may represent the PUMA-b form. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 Antibody (ABIN500557) Images back to top
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BBC3 Antibody (ABIN500557)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Background publications

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001

Yu, Zhang, Hwang, Kinzler, Vogelstein: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001

Han, Flemington, Houghton, Gu, Zambetti, Lutz, Zhu, Chittenden: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN500557) back to top
Supplier Images
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN500557) Immunocytochemistry of PUMA in K562 cells with this product at 1 µg/ml.
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN500557) Immunofluorescence of PUMA in K562 cells with this product at 2 µg/ml.
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN500557) Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lys...