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anti-Rat (Rattus) BBC3 antibody for Immunofluorescence

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • JFY-1
  • JFY1
  • Puma
  • PUMA
  • PUMA/JFY1
Epitope
C-Term
95
53
21
15
13
13
13
11
8
4
3
3
3
2
2
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
275
81
57
2
1
1
Host
Rabbit
243
28
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
13
10
8
8
8
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
262
84
66
53
43
34
11
9
6
4
3
2
2
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Catalog No. ABIN264891
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010840179 ABIN264892 IF WB Rabbit IgG C-Term Log in to see Polyclonal 3

Top referenced anti-BBC3 antibody for Immunofluorescence

Similar anti-BBC3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 262 Antibodies 70 Antibodies 45 Antibodies
Immunoprecipitation (IP) 4 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 12 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 67 Antibodies 7 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope C-Term
(95), (53), (21), (15), (13), (13), (13), (11), (8), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(275), (81), (57), (2), (1), (1)
Host Rabbit
(243), (28), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(13), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(262), (84), (66), (53), (43), (34), (11), (9), (6), (4), (3), (2), (2)
Pubmed 3 references available
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling References for anti-BBC3 Antibody (ABIN264891) Images
Specificity This antibody detects a 23 kDa protein corresponding to the molecular mass of PUMA on SDS PAGE immunoblots. 16 kDa band may be seen in some instances, possibly corresponding to PUMAβ.
Purification Affinity Chromatography
Immunogen C-terminal amino acids of Human PUMA
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling References for anti-BBC3 Antibody (ABIN264891) Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Gene ID 27113
NCBI Accession NP_001120712
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling References for anti-BBC3 Antibody (ABIN264891) Images back to top
Application Notes Western blot: 1: 1000. Immunoflourescence: 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details References for anti-BBC3 Antibody (ABIN264891) Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-BBC3 Antibody (ABIN264891)

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling Images back to top
Background publications

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001

Yu, Zhang, Hwang, Kinzler, Vogelstein: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001

Han, Flemington, Houghton, Gu, Zambetti, Lutz, Zhu, Chittenden: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001