anti-Rat (Rattus) BBC3 antibody for Immunoprecipitation

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
  • BCL2 binding component 3
  • Bcl-2 binding component 3
  • BBC3
  • Bbc3
  • bbc3
Epitope
AA 76-170
93
62
19
18
16
12
10
9
8
8
5
5
3
2
2
2
1
1
1
1
1
1
1
Reactivity
Rat (Rattus)
294
120
106
18
1
1
1
Host
Rabbit
260
24
15
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
14
12
10
9
9
8
4
4
4
4
4
4
4
4
4
4
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
276
85
77
59
49
32
20
13
8
6
2
1
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Catalog No. ABIN1870134
$ 362.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1493084 IP WB Sepharose Rabbit C-Term Log in to see Polyclonal
1 ABIN2880419 IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN1690536 IP Sepharose Rabbit Log in to see Polyclonal
1 ABIN1690188 IP Magnetic Particles Rabbit Log in to see Polyclonal

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Epitope AA 76-170
(93), (62), (19), (18), (16), (12), (10), (9), (8), (8), (5), (5), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Rat (Rattus)
(294), (120), (106), (18), (1), (1), (1)
Host Rabbit
(260), (24), (15)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(14), (12), (10), (9), (9), (8), (4), (4), (4), (4), (4), (4), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blotting (WB)
(276), (85), (77), (59), (49), (32), (20), (13), (8), (6), (2), (1)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Specificity The antibody is a rabbit polyclonal antibody raised against PUMA. It has been selected for its ability to recognize PUMA in immunohistochemical staining and western blotting.
Purification Antigen-specific affinity chromatography
Immunogen PUMA (Arg76-Val170)
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name p53 Upregulated Modulator Of Apoptosis (PUMA) (BBC3 Antibody Abstract)
Background Alternative Names: JFY1, BBC3, BCL2 Binding Component 3
Pathways p53 Signaling, Positive Regulation of Endopeptidase Activity

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes
  • Western blotting: 1:50-400 Immunocytochemistry in formalin fixed cells: 1:50-500 Immunohistochemistry in formalin fixed frozen section: 1:50-500 Immunohistochemistry in paraffin section: 1:10-100 Enzyme-linked Immunosorbent Assay: 1:100-1:5000 Optimal working dilutions must be determined by end user.
Comment

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Format Liquid
Concentration 200 μg/mL
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C
Storage Comment Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.
Expiry Date 12 months

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BCL2 Binding Component 3 (BBC3) (AA 76-170) antibody (ABIN1870134) Figure. Western Blot; Sample: Recombinant PUMA, Rat.
 image for anti-BCL2 Binding Component 3 (BBC3) (AA 76-170) antibody (ABIN1870134) Figure.DAB staining on IHC-P. Samples: Rat Tissue