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anti-Rat (Rattus) BBC3 antibody for Western Blotting

Recommended BBC3 Antibody (supplied by: Log in to see )

Antigen
BCL2 Binding Component 3 (BBC3) Antibodies
  • BBC3
  • JFY-1
  • JFY1
  • Puma
  • PUMA
  • PUMA/JFY1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
273
79
57
2
1
1
Host
Rabbit
241
28
14
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
13
10
8
8
8
6
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
260
84
64
52
43
33
11
9
6
4
3
2
2
Supplier
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Catalog No. ABIN411574
$ 283.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13476625 ABIN492517 EIA WB Mouse IgG1 AA 76-170 Log in to see 2A8F6
0.13476625 ABIN492519 EIA WB Mouse IgG1 AA 76-170 Log in to see 10C5G1
0.13476625 ABIN492518 EIA WB Mouse IgG1 AA 76-170 Log in to see 2A9G5
0.13476625 ABIN492520 EIA WB Mouse IgG1 AA 76-170 Log in to see 10D4G7
0.13476625 ABIN3042633 WB Rabbit IgG AA 145-159, C-Term Log in to see Polyclonal
0.13476625 ABIN1871229 WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN3186602 ELISA WB Rabbit IgG C-Term Log in to see Polyclonal
0.13476625 ABIN2425609 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2431101 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13476625 ABIN2498248 ELISA WB Mouse IgG1 Log in to see 2A8F6
0.13476625 ABIN1031787 ELISA WB Mouse Log in to see 10C5G1
0.13476625 ABIN1031785 ELISA WB Mouse Log in to see 2A8F6
0.13476625 ABIN1031786 ELISA WB Mouse Log in to see 2A9G5
0.13476625 ABIN1031788 ELISA WB Mouse Log in to see 10D4G7
0.13476625 ABIN2498247 ELISA WB Mouse Log in to see 2A9G5
0.13476625 ABIN2498249 ELISA WB Mouse Log in to see 10C5G1
0.13476625 ABIN2498250 ELISA WB Mouse Log in to see 10D4G7
0.0011760078 ABIN731528 IF (p) IHC (p) WB Rabbit IgG AA 120-170 Log in to see Polyclonal 1
0.0011760078 ABIN264891 IF WB Rabbit IgG C-Term Log in to see Polyclonal 3
0.0011760078 ABIN264892 IF WB Rabbit IgG C-Term Log in to see Polyclonal 3

Top referenced anti-BBC3 antibody for Western Blotting

Similar anti-BBC3 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 262 Antibodies 70 Antibodies 45 Antibodies
Immunoprecipitation (IP) 4 Antibodies 2 Antibodies 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 34 Antibodies 12 Antibodies 5 Antibodies
Immunohistochemistry (IHC) 67 Antibodies 7 Antibodies 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies 1 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies 11 Antibodies

General

Antigen BCL2 Binding Component 3 (BBC3) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(273), (79), (57), (2), (1), (1)
Host Rabbit
(241), (28), (14)
Clonality
Polyclonal
Conjugate This BBC3 antibody is un-conjugated
(13), (10), (8), (8), (8), (6), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(260), (84), (64), (52), (43), (33), (11), (9), (6), (4), (3), (2), (2)
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Product Details anti-BBC3 Antibody

Target Details BBC3 Application Details Handling Images
Blocking Peptide Blocking peptide for this product available: ABIN412890
Specificity The antibody detects ~25 kDa (193 a.a.) PUMA alpha and ~16 kDa (131 a.a.) PUMA beta in samples from human, mouse and rat origins.
Purification Affinity purified
Immunogen Synthetic peptide surrounding amino acid 177 of human PUMA
Isotype IgG

Target Details BBC3

Product Details anti-BBC3 Antibody Application Details Handling Images back to top
Antigen
Alternative Name PUMA (BBC3 Antibody Abstract)
Background PUMA (p53 upregulated modulator of apoptosis) is one of the pro-apoptotic Bcl-2 family members which are also transcriptional targets of p53. PUMA gene encodes two BH3 domain-containing proteins termed PUMA-α and PUMA-β. PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Synonyms: PUMA/JFY1 ,JFY-1, PUMA, BBC3, bcl-2 binding component 3
Gene ID 27113
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling

Application Details

Product Details anti-BBC3 Antibody Target Details BBC3 Handling Images back to top
Application Notes Western blotting (0.5-4 μg/mL) and Immunohistochemistry (5 μg/mL).
However, the optimal conditions should be determined individually.
Comment

Suggested positive control: Western Blot: Jurkat cells and Rat kidney tissue, IHC: Tonsil tissue
Country of Animal Origin: China

Restrictions For Research Use only

Handling

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer 100 μg (0.5 mg/mL) affinity purified rabbit anti-PUMA polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 30 % glycerol, 0.5 % BSA, 0.01 % thimerosal.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice The antibody solution should be gently mixed before use.
Avoid freeze-thaw cycles.
Storage -20 °C
Storage Comment For long-term storage, aliquot and freeze at -70 °C.
Expiry Date 12 months

Images

Product Details anti-BBC3 Antibody Target Details BBC3 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (ABIN411574) anti-BCL2 Binding Component 3 (BBC3) antibody