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anti-All Species C-MYC antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • AU016757
  • bHLHe39
  • c-myc
  • c-Myc
  • C-MYC
  • cmyc
  • CMYC
  • CMyc
  • LOC100136746
  • mMyc
  • MRTL
  • MYC
  • Myc2
  • MYCC
  • Niard
  • Nird
  • RNCMYC
  • zc-myc
Epitope
C-Term
93
66
58
40
36
34
27
25
21
17
14
14
13
13
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, All Species
638
189
151
10
8
6
5
5
4
3
3
3
3
3
2
1
1
1
1
1
Host
Mouse
426
201
16
13
8
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is un-conjugated
40
32
28
15
14
12
4
4
4
4
4
4
4
4
4
3
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
428
230
199
114
103
91
70
44
41
36
22
11
10
10
7
6
4
4
2
2
2
Supplier
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Catalog No. ABIN302092
$ 254.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008985059 ABIN302017 FACS IHC (p) IP WB FITC Mouse IgG1 C-Term Log in to see 9-00E-10 15
0.0008985059 ABIN2749043 FACS IP IHC (p) WB Biotin Mouse IgG1 C-Term Log in to see 9E10 15

Top referenced anti-C-MYC antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-C-MYC Antibodies

Application / Reactivity Human
Chromatin Immunoprecipitation (ChIP) 4 Antibodies
Dot Blot (DB) 36 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 2 Antibodies
ELISA 212 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 72 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 10 Antibodies
Immunocytochemistry (ICC) 38 Antibodies
Immunofluorescence (IF) 113 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies
Immunohistochemistry (IHC) 197 Antibodies
Immunohistochemistry (Acetone-fixed) (IHC (af)) 7 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 6 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 21 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 91 Antibodies

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope C-Term
(93), (66), (58), (40), (36), (34), (27), (25), (21), (17), (14), (14), (13), (13), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, All Species
(638), (189), (151), (10), (8), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Mouse
(426), (201), (16), (13), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is un-conjugated
(40), (32), (28), (15), (14), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(428), (230), (199), (114), (103), (91), (70), (44), (41), (36), (22), (11), (10), (10), (7), (6), (4), (4), (2), (2), (2)
Pubmed 14 references available
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling References for anti-C-MYC Antibody (ABIN302092) Images
Specificity The antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9-00E-10
Isotype IgG1

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC Antibody (ABIN302092) Images back to top
Antigen
Alternative Name c-Myc (MYC Antibody Abstract)
Target Type Tag
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC Antibody (ABIN302092) Images back to top
Application Notes Flow Cytometry: Recommended dilution: 1-5 µg/mL
Application note: Membrane permeabilization is required.
Immunoprecipitation: Recommended dilution: 1-5 µg/mL
Application note: not suitable for immunoprecipitation of native c-Myc protein
Western Blotting: Recommended dilution: 0,5-2 µg/mL
Positive control: c-Myc tagged proteinImmunohistochemistry (paraffin sections) Recommended dilution: 5-10 µg/mL
Positive tissue: perfused brain sections, liver, spleen

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC Antibody (ABIN302092) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-C-MYC Antibody (ABIN302092)

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Product cited in:

Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014

Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008

Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004

Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002

Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999

Background publications

Hoffman, Amanullah, Shafarenko, Liebermann: "The proto-oncogene c-myc in hematopoietic development and leukemogenesis." in: Oncogene, Vol. 21, Issue 21, pp. 3414-21, 2002

Boxer, Dang: "Translocations involving c-myc and c-myc function." in: Oncogene, Vol. 20, Issue 40, pp. 5595-610, 2001

Prendergast: "Mechanisms of apoptosis by c-Myc." in: Oncogene, Vol. 18, Issue 19, pp. 2967-87, 1999

Dang, Resar, Emison, Kim, Li, Prescott, Wonsey, Zeller: "Function of the c-Myc oncogenic transcription factor." in: Experimental cell research, Vol. 253, Issue 1, pp. 63-77, 1999

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling References for anti-C-MYC Antibody (ABIN302092) back to top
Supplier Images
 image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (C-Term) (ABIN302092) Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)