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anti-All Species C-MYC antibody for Immunoprecipitation

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • LOC100136746
  • RNCMYC
  • c-myc
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • bHLHe39
  • MYC
  • cmyc
  • zc-myc
  • MRTL
  • MYCC
  • c-Myc
  • C-MYC
  • CMYC
  • CMyc
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • myelocytomatosis oncogene a
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • LOC100136746
  • Myc
  • myca
  • MYC
Epitope
C-Term
145
120
75
38
34
25
21
15
15
14
12
10
9
9
9
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, All Species
630
213
124
48
42
36
20
18
18
14
14
13
12
9
9
6
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Host
Mouse
352
291
29
14
8
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is un-conjugated
42
36
35
17
16
16
13
12
12
12
12
12
12
11
11
9
9
7
6
5
5
5
4
4
4
4
4
4
4
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
423
227
220
212
210
146
139
119
89
52
18
14
13
11
10
6
6
5
4
2
2
1
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Catalog No. ABIN302092
$ 254.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN302017 FACS IHC (p) IP WB FITC Mouse IgG1 C-Term Log in to see 9E10 15
1 ABIN4285695 ELISA FACS ICC IF IP WB Mouse IgG1 Log in to see Myc-A7
1 ABIN2749043 FACS IP IHC (p) WB Biotin Mouse IgG1 C-Term Log in to see 9E10 15
1 ABIN3199353 IHC (p) IP WB HRP Mouse IgG1 C-Term Log in to see 9E10
1 ABIN4965083 IF IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN4994828 IHC IHC (p) IP WB HRP Mouse IgG1 Log in to see PL14 6
1 ABIN4957393 ICC IF IP WB Mouse IgG1 Log in to see PL14
1 ABIN3040346 FACS IP IHC WB Biotin Mouse IgG1 C-Term Log in to see
1 ABIN3040344 FACS IP IHC WB Mouse IgG1 C-Term Log in to see
1 ABIN3040347 FACS IP IHC WB FITC Mouse IgG1 C-Term Log in to see
1 ABIN3040345 FACS IP IHC WB Mouse IgG1 C-Term Log in to see

Top referenced anti-C-MYC antibody for Immunoprecipitation

Similar anti-C-MYC Antibodies

Application / Reactivity Human
ChIP DNA-Sequencing (ChIPSeq) 1 Antibodies
Chromatin Immunoprecipitation (ChIP) 5 Antibodies
Cytometry by Time of Flight (CyTOF) 11 Antibodies
Dot Blot (DB) 18 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 2 Antibodies
ELISA 186 Antibodies
Enzyme Immunoassay (EIA) 10 Antibodies
Flow Cytometry (FACS) 196 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Immunochromatography (IC) 14 Antibodies
Immunocytochemistry (ICC) 111 Antibodies
Immunoelectron Microscopy (IEM) 4 Antibodies
Immunofluorescence (IF) 177 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 13 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 137 Antibodies

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope C-Term
(145), (120), (75), (38), (34), (25), (21), (15), (15), (14), (12), (10), (9), (9), (9), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, All Species
(630), (213), (124), (48), (42), (36), (20), (18), (18), (14), (14), (13), (12), (9), (9), (6), (4), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1)
Host Mouse
(352), (291), (29), (14), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is un-conjugated
(42), (36), (35), (17), (16), (16), (13), (12), (12), (12), (12), (12), (12), (11), (11), (9), (9), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(423), (227), (220), (212), (210), (146), (139), (119), (89), (52), (18), (14), (13), (11), (10), (6), (6), (5), (4), (2), (2), (1)
Pubmed 14 references available
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling References for anti-C-MYC Antibody (ABIN302092) Images
Specificity The antibody 9E10 may be used to detect the c-Myc tag.
The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.
Purification Purified by protein A
Purity > 95 % (by SDS-PAGE)
Immunogen Synthetic peptide sequence (AEEQKLISEEDLL) corresponding to the C-terminal region of human c-Myc.
Clone 9E10
Isotype IgG1

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC Antibody (ABIN302092) Images back to top
Antigen
Alternative Name c-Myc (MYC Antibody Abstract)
Research Area Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC Antibody (ABIN302092) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC Antibody (ABIN302092) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.

References for anti-C-MYC Antibody (ABIN302092)

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Product cited in:

Jelínková, Šafaříková, Vondálová Blanářová, Skender, Hofmanová, Sova, Moyer, Kozubík, Kolář, Ehrmann, Hyršlová Vaculová: "Platinum(IV) complex LA-12 exerts higher ability than cisplatin to enhance TRAIL-induced cancer cell apoptosis via stimulation of mitochondrial pathway." in: Biochemical pharmacology, Vol. 92, Issue 3, pp. 415-24, 2014

Veracini, Simon, Richard, Schraven, Horejsi, Roche, Benistant: "The Csk-binding protein PAG regulates PDGF-induced Src mitogenic signaling via GM1." in: The Journal of cell biology, Vol. 182, Issue 3, pp. 603-14, 2008

Wang, Campoli, Ko, Luo, Ferrone: "Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti-tag monoclonal antibodies." in: Journal of immunological methods, Vol. 294, Issue 1-2, pp. 23-35, 2004

Fujiwara, Poikonen, Aleman, Valtavaara, Saksela, Mayer: "A single-chain antibody/epitope system for functional analysis of protein-protein interactions." in: Biochemistry, Vol. 41, Issue 42, pp. 12729-38, 2002

Baggio, Burgstaller, Hale, Putney, Lane, Lipovsek, Wright, Roberts, Liu, Szostak, Wagner: "Identification of epitope-like consensus motifs using mRNA display." in: Journal of molecular recognition : JMR, Vol. 15, Issue 3, pp. 126-34, 2002

Cermák, Símová, Pintzas, Horejsí, Andera: "Molecular mechanisms involved in CD43-mediated apoptosis of TF-1 cells. Roles of transcription Daxx expression, and adhesion molecules." in: The Journal of biological chemistry, Vol. 277, Issue 10, pp. 7955-61, 2002

Hilpert, Hansen, Wessner, Küttner, Welfle, Seifert, Höhne: "Anti-c-myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose." in: Protein engineering, Vol. 14, Issue 10, pp. 803-6, 2001

Siegel, Brandner, Hess: "Cross-reactivity of the monoclonal antibody 9E10 with murine c-MYC." in: International journal of oncology, Vol. 13, Issue 6, pp. 1259-62, 1999

Background publications

Hoffman, Amanullah, Shafarenko, Liebermann: "The proto-oncogene c-myc in hematopoietic development and leukemogenesis." in: Oncogene, Vol. 21, Issue 21, pp. 3414-21, 2002

Boxer, Dang: "Translocations involving c-myc and c-myc function." in: Oncogene, Vol. 20, Issue 40, pp. 5595-610, 2001

Prendergast: "Mechanisms of apoptosis by c-Myc." in: Oncogene, Vol. 18, Issue 19, pp. 2967-87, 1999

Dang, Resar, Emison, Kim, Li, Prescott, Wonsey, Zeller: "Function of the c-Myc oncogenic transcription factor." in: Experimental cell research, Vol. 253, Issue 1, pp. 63-77, 1999

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling References for anti-C-MYC Antibody (ABIN302092) back to top
Supplier Images
 image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (C-Term) (ABIN302092) Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)