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anti-Human C-MYC antibody for Chromatin Immunoprecipitation

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • AU016757
  • bHLHe39
  • c-myc
  • c-Myc
  • C-MYC
  • cmyc
  • CMYC
  • CMyc
  • LOC100136746
  • mMyc
  • MRTL
  • MYC
  • Myc2
  • MYCC
  • Niard
  • Nird
  • RNCMYC
  • zc-myc
Epitope
Center
94
66
58
40
36
34
27
25
21
17
14
14
13
13
5
4
4
4
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
638
188
151
10
9
6
5
5
4
3
3
3
3
3
2
1
1
1
1
1
Host
Rabbit
425
202
16
13
8
1
Clonality
Polyclonal
Conjugate
This C-MYC antibody is un-conjugated
40
32
28
15
14
12
4
4
4
4
4
4
4
4
4
3
1
1
1
Application
Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
428
230
199
113
103
92
71
44
40
36
22
11
10
10
7
6
4
3
2
2
2
Supplier
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Available images

Catalog No. ABIN2855631
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008873667 ABIN1873793 ChIP IP WB Rabbit IgG Log in to see Polyclonal
0.0008873667 ABIN2698879 ChIP IP WB Rabbit IgG Log in to see Polyclonal
0.0008873667 ABIN2988666 ChIP IP WB Rabbit IgG Log in to see Polyclonal

Similar anti-C-MYC Antibodies

Application / Reactivity Mouse (Murine) Human
Chromatin Immunoprecipitation (ChIP) 3 Antibodies 4 Antibodies
ELISA 41 Antibodies 212 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 10 Antibodies
Immunocytochemistry (ICC) 14 Antibodies
Immunofluorescence (IF) 36 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 79 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 15 Antibodies
Western Blotting (WB) 122 Antibodies
Dot Blot (DB) 36 Antibodies
Electrophoretic Mobility-Shift Assay (EMSA) 2 Antibodies

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope Center
(94), (66), (58), (40), (36), (34), (27), (25), (21), (17), (14), (14), (13), (13), (5), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(638), (188), (151), (10), (9), (6), (5), (5), (4), (3), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(425), (202), (16), (13), (8), (1)
Clonality
Polyclonal
Conjugate This C-MYC antibody is un-conjugated
(40), (32), (28), (15), (14), (12), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (1), (1), (1)
Application Chromatin Immunoprecipitation (ChIP), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(428), (230), (199), (113), (103), (92), (71), (44), (40), (36), (22), (11), (10), (10), (7), (6), (4), (3), (2), (2), (2)
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling Images
Cross-Reactivity Chimpanzee
Cross-Reactivity (Details) Chimpanzee (100 %)
Characteristics Rabbit Polyclonal antibody to c-Myc (v-myc myelocytomatosis viral oncogene homolog (avian))
c-Myc antibody
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human c-Myc. The exact sequence is proprietary.
Isotype IgG

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling Images back to top
Antigen
Alternative Name C-Myc (MYC Antibody Abstract)
Background The protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.

Cellular Localization: Nucleus
Molecular Weight 49 kDa
Gene ID 4609
Research Area Cancer, Cell Cycle, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling Images back to top
Application Notes Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , NIH-3T3 , Raw264.7 , HeLa , HeLa nuclear extract

Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling back to top
Supplier Images
Chromatin Immunoprecipitation (ChIP) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (Center) (ABIN2855631) ChIP Image c-Myc antibody immunoprecipitates c-Myc protein-DNA in ChIP experiments. C...
Western Blotting (WB) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (Center) (ABIN2855631) WB Image Sample (30 ug of whole cell lysate)    A: NIH-3T3   B: Raw264.7   10% SDS PA...
Western Blotting (WB) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (Center) (ABIN2855631) WB Image Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug 10% SDS PAGE antibody d...
Immunofluorescence (IF) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (Center) (ABIN2855631) ICC/IF Image c-Myc antibody detects c-Myc protein at nucleus by immunofluorescent ana...
Immunoprecipitation (IP) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (Center) (ABIN2855631) IP Image c-Myc antibody immunoprecipitates c-Myc protein in IP experiments. IP Sample...
Western Blotting (WB) image for anti-C-MYC antibody (V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian)) (Center) (ABIN2855631) WB Image c-Myc antibody detects c-Myc protein by western blot analysis. HeLa whole ce...