anti-Human C-MYC antibody for Enzyme Immunoassay

Recommended C-MYC Antibody (supplied by: Log in to see )

Antigen
V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
  • LOC100136746
  • RNCMYC
  • c-myc
  • mMyc
  • AU016757
  • Myc2
  • Niard
  • Nird
  • bHLHe39
  • MYC
  • cmyc
  • zc-myc
  • MRTL
  • MYCC
  • c-Myc
  • C-MYC
  • CMYC
  • CMyc
  • v-myc myelocytomatosis viral oncogene homolog
  • myelocytomatosis oncogene
  • myelocytomatosis oncogene a
  • v-myc avian myelocytomatosis viral oncogene homolog
  • v-myc myelocytomatosis viral oncogene homolog (avian)
  • LOC100136746
  • Myc
  • myca
  • MYC
Epitope
C-Term
148
121
77
46
43
29
26
16
15
15
14
10
5
5
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
671
215
125
50
42
36
20
20
18
14
14
13
12
9
9
6
6
4
4
4
3
3
3
2
2
1
1
1
1
1
1
Host
Mouse
377
308
29
16
8
1
Clonality
Monoclonal
Conjugate
This C-MYC antibody is conjugated to HRP
45
39
37
19
17
17
14
13
13
13
13
13
13
12
12
10
10
8
6
5
5
5
4
4
4
4
4
4
4
2
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
448
244
229
228
227
160
150
124
91
52
18
14
14
11
9
8
6
6
4
2
2
1
Supplier
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Catalog No. ABIN119683
$ 434.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358293 EIA WB Rabbit Ig pThr58 Log in to see Polyclonal 5
1 ABIN119684 EIA FACS IHC (fro) IHC (p) IP WB Mouse IgG1 C-Term Log in to see 9-00E-010 2
1 ABIN320077 EIA IHC (fro) IHC (p) WB Alkaline Phosphatase (AP) Mouse IgG1 C-Term Log in to see 9-00E-010 7
1 ABIN320079 EIA FACS IHC (fro) IHC (p) WB Mouse IgG1 C-Term Log in to see 9-00E-010 2
1 ABIN5610922 EIA IHC (p) WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN5610924 EIA WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN5610923 EIA WB Rabbit Ig Fraction Log in to see Polyclonal
1 ABIN950700 EIA IHC (fro) IF WB FITC Chicken Ig Log in to see Polyclonal
1 ABIN950702 EIA IHC (fro) IF IP WB Chicken Ig Log in to see Polyclonal

General

Antigen V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies
Epitope C-Term
(148), (121), (77), (46), (43), (29), (26), (16), (15), (15), (14), (10), (5), (5), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(671), (215), (125), (50), (42), (36), (20), (20), (18), (14), (14), (13), (12), (9), (9), (6), (6), (4), (4), (4), (3), (3), (3), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(377), (308), (29), (16), (8), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This C-MYC antibody is conjugated to HRP
(45), (39), (37), (19), (17), (17), (14), (13), (13), (13), (13), (13), (13), (12), (12), (10), (10), (8), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (2), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(448), (244), (229), (228), (227), (160), (150), (124), (91), (52), (18), (14), (14), (11), (9), (8), (6), (6), (4), (2), (2), (1)
Pubmed 2 references available
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Product Details anti-C-MYC Antibody

Target Details C-MYC Application Details Handling References for anti-C-MYC antibody (ABIN119683) Images
Sequence AEEQKLISEE DLL
Specificity This antibody detects the 62 kDa c-myc gene product. SM1863HRP may also be used to detect the commonly used c-myc 'tag'.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity Chromatography on Protein G
Immunogen Synthetic peptide sequence corresponding to the C-terminal region of Human c-myc.
Clone 9-00E-010
Isotype IgG1

Target Details C-MYC

Product Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC antibody (ABIN119683) Images back to top
Antigen
Alternative Name C-Myc (MYC Antibody Abstract)
Background C-myc gene product is involved in the regulation of the cell cycle and cell growth. It is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag
Gene ID 4609
NCBI Accession NP_002458
UniProt P01106
Research Area Transcription Factors, Cancer, Cell Cycle
Pathways p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus

Application Details

Product Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC antibody (ABIN119683) Images back to top
Application Notes ELISA: 1/100-1/500. Western blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining and does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC antibody (ABIN119683) Images back to top
Concentration 1.0 mg/mL
Buffer PBS, pH 7.4, 0.01 % Thimerosal, HRP Stabilizer
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-C-MYC antibody (ABIN119683)

Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Background publications

Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988

Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986