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|Antigen||V-Myc Myelocytomatosis Viral Oncogene Homolog (Avian) (MYC) Antibodies|
|Conjugate||This C-MYC antibody is conjugated to HRP Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|2 references available|
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Product Details anti-C-MYC AntibodyTarget Details C-MYC Application Details Handling References for anti-C-MYC antibody (ABIN119683) Images
|Specificity||This antibody detects the 62 kDa c-myc gene product. SM1863HRP may also be used to detect the commonly used c-myc 'tag'.|
|Cross-Reactivity (Details)||Species reactivity (tested):Human.|
|Purification||Affinity Chromatography on Protein G|
|Immunogen||Synthetic peptide sequence corresponding to the C-terminal region of Human c-myc.|
Target Details C-MYCProduct Details anti-C-MYC Antibody Application Details Handling References for anti-C-MYC antibody (ABIN119683) Images back to top
|Alternative Name||C-Myc (MYC Antibody Abstract)|
|Background||C-myc gene product is involved in the regulation of the cell cycle and cell growth. It is primarily located to the cell nucleus, but has also been shown to localised to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers.Synonyms: c-myc tag, myc tag, myc-tag|
|Research Area||Transcription Factors, Cancer, Cell Cycle|
|Pathways||p53 Signaling, Cell Division Cycle, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus|
Application DetailsProduct Details anti-C-MYC Antibody Target Details C-MYC Handling References for anti-C-MYC antibody (ABIN119683) Images back to top
ELISA: 1/100-1/500. Western blot: 1/100-1/500 (Non-Reducing Conditions). Detects a band of approximately 62 kDa in Colo 320HSR cell lysates. Immunohistochemistry on Frozen and Paraffin Sections: This product does not requireprotein digestion pre-treatment of paraffin sections prior to staining and does not requireantigen retrieval using heat treatment prior to staining of paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-C-MYC Antibody Target Details C-MYC Application Details References for anti-C-MYC antibody (ABIN119683) Images back to top
|Buffer||PBS, pH 7.4, 0.01 % Thimerosal, HRP Stabilizer|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-C-MYC antibody (ABIN119683)Product Details anti-C-MYC Antibody Target Details C-MYC Application Details Handling Images back to top
Spandidos, Pintzas, Kakkanas, Yiagnisis, Mahera, Patra, Agnantis: "Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue." in: Anticancer research, Vol. 7, Issue 6, pp. 1299-304, 1988
Evan, Lewis, Ramsay, Bishop: "Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product." in: Molecular and cellular biology, Vol. 5, Issue 12, pp. 3610-6, 1986