anti-Human CDKN1A antibody for Enzyme Immunoassay

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Epitope
pThr57
51
40
39
27
26
20
20
18
18
18
15
15
15
15
12
9
8
7
7
5
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
Reactivity
Human
590
184
124
30
23
9
6
5
4
2
1
1
1
1
1
1
1
1
Host
Rabbit
319
311
3
1
Clonality
Polyclonal
Conjugate
This CDKN1A antibody is un-conjugated
33
32
32
23
17
17
15
15
13
13
10
10
10
10
10
10
10
8
8
7
7
7
6
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
358
223
195
167
153
141
67
62
60
53
30
15
7
6
6
2
1
1
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Catalog No. ABIN358182
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358181 EIA IHC (p) WB Rabbit Ig pThr145 Log in to see Polyclonal 1
1 ABIN358179 DB EIA Rabbit Ig pSer130 Log in to see Polyclonal 5
1 ABIN359673 EIA IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 5
1 ABIN271762 EIA WB Rabbit Log in to see Polyclonal
1 ABIN318089 EIA IHC (p) WB Rabbit pThr145 Log in to see Polyclonal
1 ABIN2718408 EIA WB Rabbit IgG Log in to see Polyclonal

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Epitope pThr57
(51), (40), (39), (27), (26), (20), (20), (18), (18), (18), (15), (15), (15), (15), (12), (9), (8), (7), (7), (5), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Reactivity Human
(590), (184), (124), (30), (23), (9), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Rabbit
(319), (311), (3), (1)
Clonality
Polyclonal
Conjugate This CDKN1A antibody is un-conjugated
(33), (32), (32), (23), (17), (17), (15), (15), (13), (13), (10), (10), (10), (10), (10), (10), (10), (8), (8), (7), (7), (7), (6), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(358), (223), (195), (167), (153), (141), (67), (62), (60), (53), (30), (15), (7), (6), (6), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN358182) Images
Specificity This antibody detects p21Cip1 pThr57.
Purification Protein G affinity chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding T57 of human P21CIP1.
Isotype Ig

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A antibody (ABIN358182) Images back to top
Antigen
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background This gene encodes a potent cyclin-dependent kinase inhibitor. The encoded protein binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. The expression of this gene is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation. Two alternatively spliced variants, which encode an identical protein, have been reported.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Molecular Weight 17988 Da
Gene ID 1026, 9606
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A antibody (ABIN358182) Images back to top
Application Notes ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A antibody (ABIN358182) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CDKN1A antibody (ABIN358182)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Background publications

Scott, Kimura, Dong, Ichinohasama, Bergen, Kerviche, Sheridan, DeCoteau: "Methylation status of cyclin-dependent kinase inhibitor genes within the transforming growth factor beta pathway in human T-cell lymphoblastic lymphoma/leukemia." in: Leukemia research, Vol. 28, Issue 12, pp. 1293-301, 2004

Amini, Saunders, Kelley, Khalili, Sawaya: "Interplay between HIV-1 Vpr and Sp1 modulates p21(WAF1) gene expression in human astrocytes." in: The Journal of biological chemistry, Vol. 279, Issue 44, pp. 46046-56, 2004

Chen, Turner, McCarthy, Scaltriti, Bettuzzi, Yeatman: "Clusterin-mediated apoptosis is regulated by adenomatous polyposis coli and is p21 dependent but p53 independent." in: Cancer research, Vol. 64, Issue 20, pp. 7412-9, 2004

Sieburg, Tripp, Ma, Feuer: "Human T-cell leukemia virus type 1 (HTLV-1) and HTLV-2 tax oncoproteins modulate cell cycle progression and apoptosis." in: Journal of virology, Vol. 78, Issue 19, pp. 10399-409, 2004

Giraud, Hurlstone, Avril, Coqueret: "Implication of BRG1 and cdk9 in the STAT3-mediated activation of the p21waf1 gene." in: Oncogene, Vol. 23, Issue 44, pp. 7391-8, 2004

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A antibody (ABIN358182) back to top
Supplier Images
 image for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr57) antibody (ABIN358182) anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr57) antibody
 image for anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr57) antibody (ABIN358182) anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (pThr57) antibody (Image 2)