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anti-Human CDKN1A antibody for Flow Cytometry

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • cap20
  • CAP20
  • CCND1
  • CDKI
  • cdkn1
  • CDKN1
  • Cdkn1
  • CDKN1A
  • cip1
  • CIP1
  • Cip1
  • mda-6
  • MDA-6
  • mda6
  • p16Xic2
  • P21
  • p21
  • p21CIP1
  • p21Cip1
  • p21cip1
  • p21WAF
  • SDI1
  • sdi1
  • si:ch1073-48m5.2
  • UV96
  • waf1
  • Waf1
  • WAF1
Epitope
AA 1-80, N-Term
52
46
26
25
20
19
19
16
14
13
13
13
10
10
8
8
7
7
6
6
6
6
5
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Rat (Rattus)
342
107
51
2
1
1
Host
Mouse
305
80
1
1
Clonality
Monoclonal
Conjugate
This CDKN1A antibody is un-conjugated
24
24
23
15
15
15
4
4
4
4
4
4
4
4
4
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
212
150
96
72
61
45
40
39
19
12
9
7
2
1
Supplier
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Catalog No. ABIN180466
$ 531.30
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010666476 ABIN391763 FACS IF IHC (p) WB Rabbit Ig AA 134-164, C-Term Log in to see Polyclonal 5
0.0010666476 ABIN180467 FACS IHC (p) IP Mouse IgG1 Log in to see HZ52
0.0010666476 ABIN1490781 FACS IHC (p) IP WB Mouse IgG2b Log in to see CP74
0.0010666476 ABIN958082 FACS IP IHC WB Goat IgG Log in to see Polyclonal
0.0010666476 ABIN386836 FACS IHC (p) IP WB Mouse IgG2b Log in to see CP74
0.0010666476 ABIN215393 FACS IHC (fro) IF IHC (p) IP WB Mouse IgG1, kappa AA 58-77 Log in to see
0.0010666476 ABIN215392 FACS IHC (p) IP Mouse IgG1 Log in to see
0.0010666476 ABIN386834 FACS IHC (p) IP WB Biotin Mouse IgG2b Log in to see CP74
0.0010666476 ABIN386833 FACS IHC (p) IP WB Biotin Mouse IgG2b Log in to see CP74
0.0010666476 ABIN1838031 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see WA-1
0.0010666476 ABIN2326580 FACS IP IHC WB Mouse IgG2b AA 1-80 Log in to see 0-N-487
0.0010666476 ABIN1841525 FACS IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal
0.0010666476 ABIN2618949 ICC IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see WA1
0.0010666476 ABIN2326574 FACS IP IHC Mouse IgG1 Log in to see 2Q303
0.0010666476 ABIN385818 FACS IHC (p) IP Mouse IgG1 Log in to see HZ52
0.0010666476 ABIN2326653 FACS IF IP IHC WB Mouse IgG1 kappa Log in to see 3G223
0.0010666476 ABIN2326588 FACS IP IHC Mouse IgG1 Log in to see 0-N-489
0.0010666476 ABIN2739849 FACS ELISA Mouse IgG1, kappa Log in to see WA-1
0.0010666476 ABIN2326571 FACS IP IHC Mouse IgG1 Log in to see 0-N-489

Top referenced anti-CDKN1A antibody for Flow Cytometry

Similar anti-CDKN1A Antibodies

Application / Reactivity Rat (Rattus) Human
ELISA 6 Antibodies 124 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 7 Antibodies
Flow Cytometry (FACS) 2 Antibodies 20 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 9 Antibodies
Immunofluorescence (IF) 3 Antibodies 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies 45 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 12 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 28 Antibodies
Dot Blot (DB) 36 Antibodies

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Epitope AA 1-80, N-Term
(52), (46), (26), (25), (20), (19), (19), (16), (14), (13), (13), (13), (10), (10), (8), (8), (7), (7), (6), (6), (6), (6), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Rat (Rattus)
(342), (107), (51), (2), (1), (1)
Host Mouse
(305), (80), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1A antibody is un-conjugated
(24), (24), (23), (15), (15), (15), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(212), (150), (96), (72), (61), (45), (40), (39), (19), (12), (9), (7), (2), (1)
Pubmed 2 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN180466) Images
Specificity This antibody detects p21/WAF1.
Cellular Localization: Nuclear.
Characteristics Synonyms: CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1,CDK-interacting protein 1, Melanoma differentiation-associated protein 6
Purification Protein A Chromatography.
Immunogen Full length human recombinant p21 protein. Remarks: Mol. Wt. of antigen: 21 kDaEpitope: aa 1-80
Clone CP74
Isotype IgG2b

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A Antibody (ABIN180466) Images back to top
Antigen
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background P21WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. It also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Gene ID 1026
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A Antibody (ABIN180466) Images back to top
Application Notes Flow Cytometry. Immunoprecipitation (Native verified): Use Protein A, 2 μg/mg protein lysate. Western blotting: 1-2 μg/mL for 2hrs at RT. Immunohistology (Formalin/Paraffin): Use at 1/50 for 30 min. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for for 10-20 minfollowed by cooling at RT foir 20 min. Positive Control. Raji, PC12 cells, Colon carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A Antibody (ABIN180466) Images back to top
Concentration 0.2 mg/mL
Buffer 10 mM PBS, pH 7.4 containing 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-CDKN1A Antibody (ABIN180466)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Background publications

Chen, Cipriano, Sarkar, Ware, Arenkiel: "P53-independent induction of p21(waf1) pathway is preserved during tumor progression." in: International journal of oncology, Vol. 7, Issue 4, pp. 889-93, 2011

Buckley, Sweeney, Hamilton, Sini, Manning, Nicholson, deFazio, Watts, Musgrove, Sutherland: "Expression and amplification of cyclin genes in human breast cancer." in: Oncogene, Vol. 8, Issue 8, pp. 2127-33, 1993

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN180466) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (AA 1-80) (ABIN180466) anti-Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) (AA 1-80), (N-Term) antibody