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anti-Human CDKN1A antibody for Immunocytochemistry

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • cap20
  • CAP20
  • CCND1
  • CDKI
  • cdkn1
  • CDKN1
  • Cdkn1
  • CDKN1A
  • cip1
  • CIP1
  • Cip1
  • mda-6
  • MDA-6
  • mda6
  • p16Xic2
  • P21
  • p21
  • p21CIP1
  • p21Cip1
  • p21cip1
  • p21WAF
  • SDI1
  • sdi1
  • si:ch1073-48m5.2
  • UV96
  • waf1
  • Waf1
  • WAF1
Reactivity
Human, Non-Human Primate
342
107
52
2
1
Host
Mouse
305
80
1
1
Clonality
Monoclonal
Conjugate
This CDKN1A antibody is un-conjugated
24
24
23
15
15
15
4
4
4
4
4
4
4
4
4
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
212
149
96
72
61
45
40
40
20
12
8
7
2
1
Supplier
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Catalog No. ABIN94444
$ 254.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010090914 ABIN2854752 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
0.0010090914 ABIN207017 ICC ELISA WB Mouse IgG1 Log in to see WA-1
0.0010090914 ABIN2618951 ICC Rabbit pThr145 Log in to see Polyclonal
0.0010090914 ABIN2618949 ICC IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see WA1
0.0010090914 ABIN3200734 ELISA ICC IHC (p) WB Mouse IgG1 Log in to see WA-1
0.0010090914 ABIN2989744 ICC IF IHC WB Rabbit IgG Log in to see Polyclonal
0.0010090914 ABIN3039305 ICC IF IHC ELISA WB Mouse IgG1 Log in to see
0.0010090914 ABIN3059804 ICC IF IHC WB Rabbit Log in to see Polyclonal

Top referenced anti-CDKN1A antibody for Immunocytochemistry

Similar anti-CDKN1A Antibodies

Application / Reactivity Non-Human Primate Human
ELISA 1 Antibodies 124 Antibodies
Immunocytochemistry (ICC) 1 Antibodies 9 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 1 Antibodies 60 Antibodies
Western Blotting (WB) 1 Antibodies 211 Antibodies
Dot Blot (DB) 36 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 20 Antibodies
Immunofluorescence (IF) 40 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 45 Antibodies
Immunohistochemistry (IHC) 97 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 12 Antibodies
Immunoprecipitation (IP) 40 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Reactivity Human, Non-Human Primate
(342), (107), (52), (2), (1)
Host Mouse
(305), (80), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1A antibody is un-conjugated
(24), (24), (23), (15), (15), (15), (4), (4), (4), (4), (4), (4), (4), (4), (4)
Application Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), ELISA, Western Blotting (WB)
(212), (149), (96), (72), (61), (45), (40), (40), (20), (12), (8), (7), (2), (1)
Pubmed 5 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN94444) Images
Specificity The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumour suppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferating cell nuclear antigen.
Purification Purified from ascites by precipitation methods.
Purity > 95 % (by SDS-PAGE)
Immunogen Human p21 protein
Clone WA-1
Isotype IgG1

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A Antibody (ABIN94444) Images back to top
Antigen
Alternative Name CDKN1A (CDKN1A Antibody Abstract)
Background The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1, also known as Cip1, Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which is expressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors upon various stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibits Cdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA (proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21 counteracts its inhibitory activities.
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A Antibody (ABIN94444) Images back to top
Application Notes Immunohistochemistry (paraffin sections)
Positive tissue: colon carcinoma

Working concentrations should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A Antibody (ABIN94444) Images back to top
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8 °C. Do not use after expiration date stamped on vial label.

References for anti-CDKN1A Antibody (ABIN94444)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Background publications

Choi, Kim, Bae, Lim, Lee, Shin: "p21 Waf1/Cip1 expression by curcumin in U-87MG human glioma cells: role of early growth response-1 expression." in: Cancer research, Vol. 68, Issue 5, pp. 1369-77, 2008

Keeshan, Cotter, McKenna: "Bcr-Abl upregulates cytosolic p21WAF-1/CIP-1 by a phosphoinositide-3-kinase (PI3K)-independent pathway." in: British journal of haematology, Vol. 123, Issue 1, pp. 34-44, 2003

Horký, Kuchtícková, Vojtesek, Kolár: "Induction of cell-cycle inhibitor p21 in rat ventricular myocytes during early postnatal transition from hyperplasia to hypertrophy." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 46, Issue 3, pp. 233-5, 1998

Waga, Hannon, Beach, Stillman: "The p21 inhibitor of cyclin-dependent kinases controls DNA replication by interaction with PCNA." in: Nature, Vol. 369, Issue 6481, pp. 574-8, 1994

Product cited in:

Kolár, Murray, Scott, Harrison, Vojt?sek, Dusek: "Relation of Bcl-2 expression to androgen receptor, p21WAF1/CIP1, and cyclin D1 status in prostate cancer." in: Molecular pathology : MP, Vol. 53, Issue 1, pp. 15-8, 2000

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN94444) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (ABIN94444) Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with an...
Immunofluorescence (IF) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (ABIN94444) Immunofluorescence staining of p21 in human primary fibroblasts using anti-p21 (; gre...