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anti-Human CDKN1A antibody for Immunohistochemistry (Frozen Sections)

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Reactivity
Human
532
162
105
27
22
1
1
1
Host
Mouse
319
255
2
1
Clonality
Monoclonal
Conjugate
This CDKN1A antibody is un-conjugated
31
30
30
22
15
15
12
12
8
8
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
4
4
4
4
Application
Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
318
199
169
158
123
90
72
60
53
33
29
13
7
6
2
1
1
Supplier
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Catalog No. ABIN114806
$ 764.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN114807 IHC (fro) IHC (p) Mouse IgG2a Log in to see DCS-60-2
1 ABIN4342331 FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see WA-1 (HJ21) 9
1 ABIN112145 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see DCS60-2 1
1 ABIN966329 IHC (fp) IHC (fro) ELISA WB Mouse IgG1 Log in to see B459 3
1 ABIN215393 FACS IHC (fro) IF IHC (p) IP WB Mouse IgG1, kappa AA 58-77 Log in to see
1 ABIN290473 IHC (fro) IHC (p) ELISA WB Mouse Log in to see WA-1
1 ABIN1838031 IHC (fro) FACS IHC (p) ELISA WB Mouse IgG1 Log in to see WA-1
1 ABIN199523 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see
1 ABIN4342334 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 405 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342348 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 350 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342349 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 405 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342347 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 550 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342330 FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342341 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 680 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342342 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 488 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342344 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB DyLight 755 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342329 FACS ICC IF IHC IHC (fro) IHC (p) Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342335 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 488 Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342339 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Biotin Mouse IgG1 kappa Log in to see WA-1 (HJ21)
1 ABIN4342337 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see WA-1 (HJ21)

Top referenced anti-CDKN1A antibody for Immunohistochemistry (Frozen Sections)

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Application / Reactivity Human
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ELISA 143 Antibodies
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Flow Cytometry (FACS) 158 Antibodies
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Immunofluorescence (IF) 90 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 123 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
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General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Reactivity Human
(532), (162), (105), (27), (22), (1), (1), (1)
Host Mouse
(319), (255), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1A antibody is un-conjugated
(31), (30), (30), (22), (15), (15), (12), (12), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4)
Application Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(318), (199), (169), (158), (123), (90), (72), (60), (53), (33), (29), (13), (7), (6), (2), (1), (1)
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling Images
Specificity This antibody recognizes a 21 kDa protein identified as p21WAF1/Cip1/Sdi1/Pic1 tumor suppressor protein. This antibody is highly specific to p21 and does not show any cross reactivity to other mitotic inhibitors. This antibody does not cross react with mouse and rat.
Cellular Localization: Nuclear
Purification Supernatant
Immunogen BALB/C mice were injected with a full length human recombinant p21 protein.
Clone DCS-60-2
Isotype IgG2a

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling Images back to top
Antigen
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background Cell cycle progression is regulated by cyclins and their cognate Cdks. p21 (WAF1/CIP1) inhibits the activity of each member of the cyclin/Cdk family, and overexpression of this protein inhibits the proliferation of mammalian cells. The expression of p21 is inducible by a wide range of stress stimuli. p21 is tightly regulated at the transcriptional level by p53, and probably serves as the effector of p53 cell cycle control.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Gene ID 1026
NCBI Accession NP_000380
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling Images back to top
Application Notes Immunoprecipitation: 1: 50. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature)Recommended Positive Control: Colan carcinoma
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Images back to top
Format Liquid
Buffer 10 mM citrate buffer, pH 6.0 - containing sodium azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (ABIN114806) Formalin fixed paraffin embedded human colon carcinoma stained with p21WAF1 antibody....