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anti-Human CDKN1A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Epitope
C-Term
51
40
27
19
19
19
18
16
15
15
15
15
14
13
13
9
8
8
7
6
4
4
4
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
532
187
124
30
23
9
5
4
3
2
1
1
1
1
Host
Rabbit
318
256
2
1
Clonality
Polyclonal
Conjugate
This CDKN1A antibody is un-conjugated
31
30
30
22
15
15
12
12
8
8
7
7
7
7
7
7
7
7
7
6
5
5
5
4
4
4
4
4
4
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
332
203
174
158
134
92
72
61
53
31
30
13
6
6
4
2
1
1
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Catalog No. ABIN359673
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.931439 ABIN4342331 FACS ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG1 kappa Log in to see WA-1 (HJ21) 9
12.931439 ABIN4342320 ICC IF IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
12.931439 ABIN756862 IF (p) IHC (p) Rabbit IgG AA 96-146, pThr145 Log in to see Polyclonal
12.931439 ABIN757417 IF (p) IHC (p) Rabbit IgG AA 100-145, pSer130 Log in to see Polyclonal
12.931439 ABIN4342328 IHC (p) IP Mouse IgG2a Log in to see DCS-60-2
12.931439 ABIN4342317 IHC IHC (p) WB Rabbit pThr145 Log in to see Polyclonal
1 ABIN180466 FACS IHC (p) IP WB Mouse IgG2b AA 1-80, N-Term Log in to see CP74 2
1 ABIN358182 EIA IHC (p) WB Rabbit Ig pThr57 Log in to see Polyclonal 5
1 ABIN358181 EIA IHC (p) WB Rabbit Ig pThr145 Log in to see Polyclonal 1
1 ABIN657391 IF IHC (p) WB Rabbit Ig AA 117-146, C-Term Log in to see Polyclonal 1
1 ABIN389612 DB IHC (p) WB Rabbit Ig pThr145 Log in to see Polyclonal 1
1 ABIN391763 FACS IF IHC (p) WB Rabbit Ig AA 134-164, C-Term Log in to see Polyclonal 5
1 ABIN114806 IHC (fro) IHC (p) Mouse IgG2a Log in to see DCS-60-2
1 ABIN114807 IHC (fro) IHC (p) Mouse IgG2a Log in to see DCS-60-2
1 ABIN180467 FACS IHC (p) IP Mouse IgG1 Log in to see HZ52
1 ABIN1882179 DB IHC (p) WB Rabbit pSer146 Log in to see Polyclonal 7
1 ABIN319243 IHC (p) Rabbit pThr145 Log in to see Polyclonal 2
1 ABIN672606 IF (p) IHC (p) WB Rabbit IgG AA 120-155 Log in to see Polyclonal 4
1 ABIN112145 IHC (fro) IHC (p) WB Mouse IgG2a Log in to see DCS60-2 1
1 ABIN389613 DB IHC (p) Rabbit Ig pThr57 Log in to see Polyclonal

Top referenced anti-CDKN1A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CDKN1A Antibodies

Application / Reactivity Human
Cytometry by Time of Flight (CyTOF) 13 Antibodies
Dot Blot (DB) 36 Antibodies
ELISA 146 Antibodies
Enzyme Immunoassay (EIA) 7 Antibodies
Flow Cytometry (FACS) 158 Antibodies
Immunocytochemistry (ICC) 31 Antibodies
Immunofluorescence (IF) 92 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 53 Antibodies
Immunohistochemistry (IHC) 134 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 30 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 201 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
RNA Interference (RNAi) 1 Antibodies
Simple Western (SimWes) 6 Antibodies
Western Blotting (WB) 330 Antibodies

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Epitope C-Term
(51), (40), (27), (19), (19), (19), (18), (16), (15), (15), (15), (15), (14), (13), (13), (9), (8), (8), (7), (6), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(532), (187), (124), (30), (23), (9), (5), (4), (3), (2), (1), (1), (1), (1)
Host Rabbit
(318), (256), (2), (1)
Clonality
Polyclonal
Conjugate This CDKN1A antibody is un-conjugated
(31), (30), (30), (22), (15), (15), (12), (12), (8), (8), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (5), (5), (5), (4), (4), (4), (4), (4), (4)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(332), (203), (174), (158), (134), (92), (72), (61), (53), (31), (30), (13), (6), (6), (4), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN359673) Images
Specificity This antibody reacts to p21 (CDKN1A).
Purification Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDKN1A.
Isotype Ig

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A Antibody (ABIN359673) Images back to top
Antigen
Alternative Name CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)
Background CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1
Gene ID 1026, 9606
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A Antibody (ABIN359673) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A Antibody (ABIN359673) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CDKN1A Antibody (ABIN359673)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Background publications

Fukuchi, Nakamura, Ichimura, Tatsumi, Gomi: "The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif." in: Biochimica et biophysica acta, Vol. 1642, Issue 3, pp. 163-71, 2003

Frouin, Maga, Denegri, Riva, Savio, Spadari, Prosperi, Scovassi: "Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39265-8, 2003

Dupont, Karas, LeRoith: "The cyclin-dependent kinase inhibitor p21CIP/WAF is a positive regulator of insulin-like growth factor I-induced cell proliferation in MCF-7 human breast cancer cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37256-64, 2003

Di Padova, Bruno, De Nicola, Iezzi, DAngelo, Gallo, Nicosia, Corbi, Biroccio, Floridi, Passananti, Fanciulli: "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36496-504, 2003

Bai, Miyake, Iwai, Yuasa: "CDX2, a homeobox transcription factor, upregulates transcription of the p21/WAF1/CIP1 gene." in: Oncogene, Vol. 22, Issue 39, pp. 7942-9, 2003

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN359673) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (C-Term) (ABIN359673) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary ant...
Western Blotting (WB) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (C-Term) (ABIN359673) The anti-CDKN1A Pab is used in Western blot to detect CDKN1A in T-47D cell lysate.