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|Application / Reactivity||Human|
|Dot Blot (DB)||36 Antibodies|
|Enzyme Immunoassay (EIA)||7 Antibodies|
|Flow Cytometry (FACS)||20 Antibodies|
|Immunocytochemistry (ICC)||9 Antibodies|
|Immunofluorescence (IF)||40 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||46 Antibodies|
|Immunohistochemistry (IHC)||97 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||12 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||60 Antibodies|
|Immunoprecipitation (IP)||40 Antibodies|
|Proximity Ligation Assay (PLA)||2 Antibodies|
|Western Blotting (WB)||211 Antibodies|
|Antigen||Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies|
|Conjugate||This CDKN1A antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CDKN1A AntibodyTarget Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN359673) Images
|Specificity||This antibody reacts to p21 (CDKN1A).|
|Purification||Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CDKN1A.|
Target Details CDKN1AProduct Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A Antibody (ABIN359673) Images back to top
|Alternative Name||CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)|
|Background||CDKN1A is a potent cyclin-dependent kinase inhibitor. It binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at G1. Expression is tightly controlled by the tumor suppressor protein p53, through which this protein mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. This protein can interact with proliferating cell nuclear antigen (PCNA), a DNA polymerase accessory factor, and plays a regulatory role in S phase DNA replication and DNA damage repair. This protein was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1|
|Gene ID||1026, 9606|
|Pathways||p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A Antibody (ABIN359673) Images back to top
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A Antibody (ABIN359673) Images back to top
|Buffer||PBS with 0.09 % (W/V) sodium azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CDKN1A Antibody (ABIN359673)Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Fukuchi, Nakamura, Ichimura et al.: "The association of cyclin A and cyclin kinase inhibitor p21 in response to gamma-irradiation requires the CDK2 binding region, but not the Cy motif." in: Biochimica et biophysica acta, Vol. 1642, Issue 3, pp. 163-71, 2003 (PubMed).
Bai, Miyake, Iwai et al.: "CDX2, a homeobox transcription factor, upregulates transcription of the p21/WAF1/CIP1 gene." in: Oncogene, Vol. 22, Issue 39, pp. 7942-9, 2003 (PubMed).
Frouin, Maga, Denegri et al.: "Human proliferating cell nuclear antigen, poly(ADP-ribose) polymerase-1, and p21waf1/cip1. A dynamic exchange of partners." in: The Journal of biological chemistry, Vol. 278, Issue 41, pp. 39265-8, 2003 (PubMed).
Dupont, Karas, LeRoith: "The cyclin-dependent kinase inhibitor p21CIP/WAF is a positive regulator of insulin-like growth factor I-induced cell proliferation in MCF-7 human breast cancer cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37256-64, 2003 (PubMed).
Di Padova, Bruno, De Nicola et al.: "Che-1 arrests human colon carcinoma cell proliferation by displacing HDAC1 from the p21WAF1/CIP1 promoter." in: The Journal of biological chemistry, Vol. 278, Issue 38, pp. 36496-504, 2003 (PubMed).
ImagesProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN359673) back to top