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|Application / Reactivity||Rat (Rattus)||Human|
|ELISA||6 Antibodies||124 Antibodies|
|Enzyme Immunoassay (EIA)||2 Antibodies||7 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||20 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||10 Antibodies|
|Immunofluorescence (IF)||3 Antibodies||41 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies||45 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||12 Antibodies|
|Immunoprecipitation (IP)||9 Antibodies|
|Western Blotting (WB)||28 Antibodies|
|Dot Blot (DB)||36 Antibodies|
|Antigen||Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies|
|Epitope||AA 1-80, N-Term Alternatives|
|Reactivity||Human, Rat (Rattus) Alternatives|
|Conjugate||This CDKN1A antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|2 references available|
|Supplier||Log in to see|
Product Details anti-CDKN1A AntibodyTarget Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN180466) Images
This antibody detects p21/WAF1.
Cellular Localization: Nuclear.
|Characteristics||Synonyms: CAP20, CDKN1, CIP1, MDA6, MDA-6, PIC1, SDI1, WAF1, Cyclin-dependent kinase inhibitor 1,CDK-interacting protein 1, Melanoma differentiation-associated protein 6|
|Purification||Protein A Chromatography.|
|Immunogen||Full length human recombinant p21 protein. Remarks: Mol. Wt. of antigen: 21 kDaEpitope: aa 1-80|
Target Details CDKN1AProduct Details anti-CDKN1A Antibody Application Details Handling References for anti-CDKN1A Antibody (ABIN180466) Images back to top
|Alternative Name||CDKN1A / p21WAF1 (CDKN1A Antibody Abstract)|
|Background||P21WAF1/Cip1/Sdi1/Pic1 is a tumor suppressor protein. Expression of p21WAF1 is induced by wild type, but not mutant, p53 suppressor protein. The p21WAF1 protein binds to cyclin/CDK complexes and inhibits their kinase activity thereby stopping cell cycle progression. It also binds to PCNA (proliferating cell nuclear antigen) and blocks DNA replication but not the DNA repair process.Synonyms: CAP20, CDK-interacting protein 1, CDKN1, CIP1, Cyclin-dependent kinase inhibitor 1, MDA-6, MDA6, Melanoma differentiation-associated protein 6, PIC1, SDI1, WAF1|
|Pathways||p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway|
Application DetailsProduct Details anti-CDKN1A Antibody Target Details CDKN1A Handling References for anti-CDKN1A Antibody (ABIN180466) Images back to top
Flow Cytometry. Immunoprecipitation (Native verified): Use Protein A, 2 μg/mg protein lysate. Western blotting: 1-2 μg/mL for 2hrs at RT. Immunohistology (Formalin/Paraffin): Use at 1/50 for 30 min. Staining of formalin-fixedtissue requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for for 10-20 minfollowed by cooling at RT foir 20 min. Positive Control. Raji, PC12 cells, Colon carcinoma.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details References for anti-CDKN1A Antibody (ABIN180466) Images back to top
|Buffer||10 mM PBS, pH 7.4 containing 0.2 % BSA as stabilizer and 0.09 % Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-CDKN1A Antibody (ABIN180466)Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Chen, Cipriano, Sarkar, Ware, Arenkiel: "P53-independent induction of p21(waf1) pathway is preserved during tumor progression." in: International journal of oncology, Vol. 7, Issue 4, pp. 889-93, 2011
Buckley, Sweeney, Hamilton, Sini, Manning, Nicholson, deFazio, Watts, Musgrove, Sutherland: "Expression and amplification of cyclin genes in human breast cancer." in: Oncogene, Vol. 8, Issue 8, pp. 2127-33, 1993
ImagesProduct Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling References for anti-CDKN1A Antibody (ABIN180466) back to top