anti-Rat (Rattus) CDKN1A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended CDKN1A Antibody (supplied by: Log in to see )

Antigen
Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
  • p21
  • cip1
  • p16Xic2
  • CCND1
  • sdi1
  • waf1
  • cap20
  • cdkn1
  • mda-6
  • p21cip1
  • CDKN1A
  • si:ch1073-48m5.2
  • Cip1
  • UV96
  • Waf1
  • CAP20
  • CDKN1
  • CIP1
  • MDA-6
  • P21
  • SDI1
  • WAF1
  • p21CIP1
  • CDKI
  • Cdkn1
  • mda6
  • p21Cip1
  • p21WAF
  • cyclin-dependent kinase inhibitor 1A (p21, Cip1)
  • cyclin-dependent kinase inhibitor 1
  • cyclin-dependent kinase inhibitor 1A
  • cyclin-dependent kinase inhibitor 1A (P21)
  • cdkn1a
  • cki1
  • CDKN1A
  • LOC100348489
  • Cdkn1a
Reactivity
Human, Mouse (Murine), Primate, Rat (Rattus)
522
185
124
30
22
10
6
5
4
2
1
1
1
1
1
1
1
1
Host
Mouse
305
257
2
1
Clonality
Monoclonal
Conjugate
This CDKN1A antibody is un-conjugated
30
29
29
22
15
15
13
13
9
9
7
7
7
7
7
7
7
7
7
6
6
6
6
4
4
4
4
4
4
Application
Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
318
204
165
164
130
91
67
60
53
30
29
13
7
6
4
2
1
1
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Catalog No. ABIN4342331
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.850952 ABIN756862 IF (p) IHC (p) Rabbit IgG AA 96-146, pThr145 Log in to see Polyclonal
13.850952 ABIN4342317 IHC IHC (p) WB Rabbit pThr145 Log in to see Polyclonal
1 ABIN180466 FACS IHC (p) IP WB Mouse IgG2b AA 1-80, N-Term Log in to see CP74 2
1 ABIN180467 FACS IHC (p) IP Mouse IgG1 Log in to see HZ52
1 ABIN672606 IF (p) IHC (p) WB Rabbit IgG AA 120-155 Log in to see Polyclonal 4
1 ABIN151440 IHC (p) IP IHC WB Rabbit C-Term Log in to see Polyclonal 3
1 ABIN1490781 FACS IHC (p) IP WB Mouse IgG2b AA 1-80 Log in to see CP74
1 ABIN181292 IHC (p) IP Rabbit AA 2-14, N-Term Log in to see Polyclonal
1 ABIN2879255 IHC (p) ELISA WB Rabbit IgG AA 111-160 Log in to see Polyclonal
1 ABIN2888492 IHC (p) ELISA WB Rabbit IgG pThr145 Log in to see Polyclonal
1 ABIN318089 EIA IHC (p) WB Rabbit pThr145 Log in to see Polyclonal
1 ABIN386836 FACS IHC (p) IP WB Mouse IgG2b AA 1-80 Log in to see CP74
1 ABIN1100726 IHC (p) IP Mouse IgG1 Log in to see HZ52
1 ABIN756871 IHC (p) HRP Rabbit IgG AA 96-146, pThr145 Log in to see Polyclonal
1 ABIN386834 FACS IHC (p) IP WB Biotin Mouse IgG2b AA 1-80 Log in to see CP74
1 ABIN386833 FACS IHC (p) IP WB Biotin Mouse IgG2b AA 1-80 Log in to see CP74
1 ABIN756864 IHC (p) Biotin Rabbit IgG AA 96-146, pThr145 Log in to see Polyclonal
1 ABIN672615 IHC (p) WB HRP Rabbit IgG AA 120-155 Log in to see Polyclonal
1 ABIN2962235 IHC (p) ELISA WB Rabbit IgG AA 111-160 Log in to see Polyclonal
1 ABIN4342337 ELISA FACS ICC IF IHC IHC (fro) IHC (p) IP WB Alexa Fluor 700 Mouse IgG1 kappa Log in to see WA-1 (HJ21)

Top referenced anti-CDKN1A antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-CDKN1A Antibodies

Application / Reactivity Rat (Rattus) Primate
Cytometry by Time of Flight (CyTOF) 4 Antibodies 2 Antibodies
ELISA 25 Antibodies 17 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Flow Cytometry (FACS) 51 Antibodies 21 Antibodies
Immunocytochemistry (ICC) 19 Antibodies 20 Antibodies
Immunofluorescence (IF) 31 Antibodies 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 44 Antibodies 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 20 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 67 Antibodies
Immunoprecipitation (IP) 32 Antibodies
RNA Interference (RNAi) 1 Antibodies
Western Blotting (WB) 64 Antibodies

General

Antigen Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A) Antibodies
Reactivity Human, Mouse (Murine), Primate, Rat (Rattus)
(522), (185), (124), (30), (22), (10), (6), (5), (4), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Host Mouse
(305), (257), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CDKN1A antibody is un-conjugated
(30), (29), (29), (22), (15), (15), (13), (13), (9), (9), (7), (7), (7), (7), (7), (7), (7), (7), (7), (6), (6), (6), (6), (4), (4), (4), (4), (4), (4)
Application Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(318), (204), (165), (164), (130), (91), (67), (60), (53), (30), (29), (13), (7), (6), (4), (2), (1), (1)
Pubmed 10 references available
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Product Details anti-CDKN1A Antibody

Target Details CDKN1A Application Details Handling ProductDetails: References for anti-CDKN1A antibody (ABIN4342331) Images
Specificity This MAb recognizes a 21 kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21 and shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdk s and a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase and is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma). In ELISA, MAb WA-1 is useful either as a solid phase or for detection of p21 protein.
Purification Protein A or G purified
Immunogen Recombinant human p21 protein was used as immunogen to generate the antibody.
Clone WA-1 (HJ21)
Isotype IgG1 kappa

Target Details CDKN1A

Product Details anti-CDKN1A Antibody Application Details Handling ProductDetails: References for anti-CDKN1A antibody (ABIN4342331) Images back to top
Antigen
Alternative Name p21/CIP1/CDKN1A (CDKN1A Antibody Abstract)
Background Gene Symbol: CDKN1A
Molecular Weight Theoretical MW: 21 kDa
Gene ID 1026
UniProt P38936
Pathways p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy

Application Details

Product Details anti-CDKN1A Antibody Target Details CDKN1A Handling ProductDetails: References for anti-CDKN1A antibody (ABIN4342331) Images back to top
Application Notes Western Blot, Flow Cytometry 0.5-1 μg/million cells, Immunohistochemistry 0.5-1 μg/mL, Immunocytochemistry/Immunofluorescence 1-2 μg/mL, Immunohistochemistry-Paraffin 0.5-1 μg/mL, Immunohistochemistry-Frozen 0.5-1 μg/mLHuman Chromosome Location: 6p21.31 Use in ELISA has been reported in literature. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in Western blot reported in scientific literature (PMID 28137862).
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details ProductDetails: References for anti-CDKN1A antibody (ABIN4342331) Images back to top
Format Liquid
Buffer 10 mM PBS and 0.05 % BSA
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store at 4°C.

ProductDetails: References for anti-CDKN1A antibody (ABIN4342331)

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling Images back to top
Product cited in:

Xiang, Yang, Xin, Gaertig, Reeves, Li, Li: "DYRK1A regulates Hap1-Dcaf7/WDR68 binding with implication for delayed growth in Down syndrome." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 114, Issue 7, pp. E1224-E1233, 2017 Method employed by authors: Western Blotting (WB) (Sample species: Mouse (Murine)).

Ge, Pettan-Brewer, Morton, Carter, Fatemi, Rabinovitch, Ladiges: "Mitochondrial catalase suppresses naturally occurring lung cancer in old mice." in: Pathobiology of aging & age related diseases, Vol. 5, pp. 28776, 2015 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry

Romanov, Pospelov, Pospelova: "Cyclin-dependent kinase inhibitor p21(Waf1): contemporary view on its role in senescence and oncogenesis." in: Biochemistry. Biokhimii͡a, Vol. 77, Issue 6, pp. 575-84, 2012

Koike, Yutoku, Koike: "Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation." in: Biochemical and biophysical research communications, Vol. 412, Issue 1, pp. 39-43, 2011

Nobre, Herráez-Hernández, Fei, Langbein, Kaden, Gröne, de Villiers: "E7 oncoprotein of novel human papillomavirus type 108 lacking the E6 gene induces dysplasia in organotypic keratinocyte cultures." in: Journal of virology, Vol. 83, Issue 7, pp. 2907-16, 2009 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Rossner, Binkova, Milcova, Solansky, Zidzik, Lyubomirova, Farmer, Sram: "Air pollution by carcinogenic PAHs and plasma levels of p53 and p21(WAF1) proteins." in: Mutation research, Vol. 620, Issue 1-2, pp. 34-40, 2007 (Sample species: Human).

Rössner, Binková, Chvátalová, Srám: "Acrylonitrile exposure: the effect on p53 and p21(WAF1) protein levels in the blood plasma of occupationally exposed workers and in vitro in human diploid lung fibroblasts." in: Mutation research, Vol. 517, Issue 1-2, pp. 239-50, 2002 Further details: Western Blotting

Scott, Ingram, Ball: "PDK1-dependent activation of atypical PKC leads to degradation of the p21 tumour modifier protein." in: The EMBO journal, Vol. 21, Issue 24, pp. 6771-80, 2002 Further details: Western Blotting

Blaydes, Luciani, Pospisilova, Ball, Vojtesek, Hupp: "Stoichiometric phosphorylation of human p53 at Ser315 stimulates p53-dependent transcription." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4699-708, 2001 Further details: Western Blotting

Uy, White, Oka: "Observation of Delta|k - l | = 3 Transitions in the nu3 Band of H3 O+" in: Journal of molecular spectroscopy, Vol. 183, Issue 2, pp. 240-4, 1997 Further details: Western Blotting

Images

Product Details anti-CDKN1A Antibody Target Details CDKN1A Application Details Handling ProductDetails: References for anti-CDKN1A antibody (ABIN4342331) back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-CDKN1A antibody (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1)) (ABIN4342331) Immunohistochemistry-Paraffin: p21/CIP1/CDKN1A Antibody (WA-1 (HJ21)) [NBP2-29463] - ...