anti-Human CHEK1 antibody for Immunofluorescence

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
pSer301
60
50
36
32
30
16
15
14
11
9
9
6
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
459
195
166
14
14
10
10
10
7
5
4
4
3
2
1
1
1
Host
Rabbit
396
54
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
389
169
134
73
64
40
22
21
21
10
7
6
6
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1531555
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
12.16076 ABIN1531554 IF ELISA WB Rabbit IgG pSer286 Log in to see Polyclonal 2
12.16076 ABIN1532570 IF ELISA WB Rabbit IgG Log in to see Polyclonal 2
12.16076 ABIN1532571 IF ELISA Rabbit IgG Log in to see Polyclonal 1
12.16076 ABIN151911 ICC IF IHC (p) IHC PLA WB Rabbit IgG AA 250-300 Log in to see Polyclonal 5
12.16076 ABIN497950 IF IHC (p) WB Rabbit AA 259-311 Log in to see Polyclonal
12.16076 ABIN2857050 ICC IF IHC (p) WB Rabbit IgG Internal Region Log in to see Polyclonal
12.16076 ABIN2857049 ICC IF WB Rabbit IgG Internal Region, Ser345 Log in to see Polyclonal
12.16076 ABIN2957528 IC IF WB Rabbit pSer301 Log in to see Polyclonal
12.16076 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.16076 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.16076 ABIN2705869 IC IF IHC WB Rabbit Center Log in to see Polyclonal
12.16076 ABIN151295 ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 2G1D5
12.16076 ABIN1870071 IF WB Rabbit IgG pSer317 Log in to see Polyclonal
12.16076 ABIN1870069 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal
12.16076 ABIN2857048 ICC IF IHC (p) WB Rabbit IgG Internal Region, pSer345 Log in to see Polyclonal
12.16076 ABIN5075918 ICC IF Rabbit IgG Log in to see Polyclonal
12.16076 ABIN2406852 IF WB Rabbit IgG pSer317 Log in to see Polyclonal
12.16076 ABIN2406850 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal
12.16076 ABIN514379 IF ELISA Mouse IgG2b kappa AA 361-476, partial Log in to see 2G3
1 ABIN439073 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal 2

Top referenced anti-CHEK1 antibody for Immunofluorescence

Similar anti-CHEK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 46 Antibodies 53 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 2 Antibodies 2 Antibodies
Immunochromatography (IC) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 7 Antibodies
Immunofluorescence (IF) 21 Antibodies 25 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 5 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 27 Antibodies
Immunohistochemistry (IHC) 49 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 31 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 117 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer301
(60), (50), (36), (32), (30), (16), (15), (14), (11), (9), (9), (6), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(459), (195), (166), (14), (14), (10), (10), (10), (7), (5), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(396), (54), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(389), (169), (134), (73), (64), (40), (22), (21), (21), (10), (7), (6), (6), (2)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images
Specificity Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Molecular weight of target protein: 54 kDa
Synonyms: CHEK1, Chk1, kinase Chk1
Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-CHEK1 antibody (ABIN1531555)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer301) (ABIN1531555) Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) Antibod...
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer301) (ABIN1531555) Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The ...