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anti-Mouse (Murine) CHEK1 antibody for Enzyme Immunoassay

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
pSer280
58
47
37
34
28
20
14
13
12
10
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
451
149
140
5
4
4
3
2
2
2
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
374
180
138
63
60
34
25
22
20
7
6
5
1
1
Supplier
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Catalog No. ABIN358108
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008867729 ABIN265347 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008867729 ABIN1106682 EIA WB Mouse IgG1 Log in to see 2G1D5 1
0.0008867729 ABIN356395 EIA WB Mouse Log in to see 2G1D5

Top referenced anti-CHEK1 antibody for Enzyme Immunoassay

Similar anti-CHEK1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 41 Antibodies 164 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 1 Antibodies 2 Antibodies
Immunochromatography (IC) 4 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 8 Antibodies 27 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 40 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 28 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 99 Antibodies
Dot Blot (DB) 22 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer280
(58), (47), (37), (34), (28), (20), (14), (13), (12), (10), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(451), (149), (140), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(374), (180), (138), (63), (60), (34), (25), (22), (20), (7), (6), (5), (1), (1)
Pubmed 5 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN358108) Images
Specificity This antibody detects CHK1 pSer280.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280 region of Human CHK1. Genename: CHEK1
Isotype Ig

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN358108) Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
Molecular Weight 54420 Da
Gene ID 1111, 9606
UniProt O14757
Research Area Kinases/Phosphatases, Cell Cycle
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN358108) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN358108) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-CHEK1 Antibody (ABIN358108)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004

Ng, Lee, Ho, Arooz, Siu, Lau, Poon: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Groth, Lukas, Nigg, Silljé, Wernstedt, Bartek, Hansen: "Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint." in: The EMBO journal, Vol. 22, Issue 7, pp. 1676-87, 2003

Chen, Ryan, Piwnica-Worms: "Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7488-97, 2003

Images

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Supplier Images
 image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer280) (ABIN358108) anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody
 image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer280) (ABIN358108) anti-Checkpoint Kinase 1 (CHEK1) (pSer280) antibody (Image 2)