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|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||37 Antibodies||158 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies||7 Antibodies|
|Flow Cytometry (FACS)||1 Antibodies||2 Antibodies|
|Immunochromatography (IC)||4 Antibodies||6 Antibodies|
|Immunocytochemistry (ICC)||8 Antibodies||25 Antibodies|
|Immunofluorescence (IF)||22 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||25 Antibodies|
|Immunohistochemistry (IHC)||33 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||21 Antibodies|
|Immunoprecipitation (IP)||3 Antibodies|
|Western Blotting (WB)||89 Antibodies|
|Dot Blot (DB)||22 Antibodies|
|Antigen||Checkpoint Kinase 1 (CHEK1) Antibodies|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This CHEK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-CHEK1 AntibodyTarget Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN358108) Images
|Specificity||This antibody detects CHK1 pSer280.|
|Purification||Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.|
|Immunogen||This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S280 region of Human CHK1. Genename: CHEK1|
Target Details CHEK1Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN358108) Images back to top
|Alternative Name||CHK1 (CHEK1 Antibody Abstract)|
|Background||Checkpoint pathways control the order and timing of cell cycle transitions and ensure that critical events, such as DNA replication and chromosome segregation, are completed with high fidelity. The S. pombe Chk1 gene encodes a protein kinase that is required for the DNA damage checkpoint. Antibodies against CHK1 recognized a 54-kD protein on immunoblots of mammalian cell extracts. However,CHK1 is modified in response to DNA damage. In vitro, CHK1 directly phosphorylated a regulator of CDC2 tyrosine phosphorylation, CDC25C. In response to DNA damage, CHK1 phosphorylates and inhibits CDC25C, thus preventing activation of the CDC2-cyclin B complex and mitotic entry. CHK1 directly phosphorylates CDC25A during an unperturbed cell cycle, and that phosphorylation of CDC25A by CHK1 is required for cells to delay cell cycle progression in response to double-strand DNA breaks.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1|
|Molecular Weight||54420 Da|
|Gene ID||1111, 9606|
|Research Area||Kinases/Phosphatases, Cell Cycle|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
Application DetailsProduct Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN358108) Images back to top
ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN358108) Images back to top
|Buffer||PBS with 0.09 % (W/V) Sodium Azide as preservative.|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.|
References for anti-CHEK1 Antibody (ABIN358108)Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Krämer, Mailand, Lukas et al.: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004 (PubMed).
Xu, Lee, Stern: "Microcephalin is a DNA damage response protein involved in regulation of CHK1 and BRCA1." in: The Journal of biological chemistry, Vol. 279, Issue 33, pp. 34091-4, 2004 (PubMed).
Ng, Lee, Ho et al.: "Differential mode of regulation of the checkpoint kinases CHK1 and CHK2 by their regulatory domains." in: The Journal of biological chemistry, Vol. 279, Issue 10, pp. 8808-19, 2004 (PubMed).
Chen, Ryan, Piwnica-Worms: "Chk1 kinase negatively regulates mitotic function of Cdc25A phosphatase through 14-3-3 binding." in: Molecular and cellular biology, Vol. 23, Issue 21, pp. 7488-97, 2003 (PubMed).
Groth, Lukas, Nigg et al.: "Human Tousled like kinases are targeted by an ATM- and Chk1-dependent DNA damage checkpoint." in: The EMBO journal, Vol. 22, Issue 7, pp. 1676-87, 2003 (PubMed).
ImagesProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN358108) back to top