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anti-Mouse (Murine) CHEK1 antibody for Immunocytochemistry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
Internal Region, pSer345
56
47
37
31
28
19
14
13
12
10
9
6
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
431
137
129
5
4
3
3
2
2
2
1
1
Host
Rabbit
377
47
21
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
12
12
11
8
8
8
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
354
173
125
55
51
34
22
20
18
8
6
4
1
1
Supplier
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Catalog No. ABIN2857048
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010161072 ABIN1858393 ICC IHC (fro) IHC (p) ELISA WB Rabbit IgG AA 194-446 Log in to see Polyclonal
0.0010161072 ABIN1843354 ICC IF WB Rabbit pSer301 Log in to see Polyclonal
0.0010161072 ABIN407619 ICC IF IHC (p) IHC WB Rabbit IgG Asp311 Log in to see Polyclonal
0.0010161072 ABIN2972901 ICC IF WB Rabbit Center, pSer345 Log in to see Polyclonal
0.0010161072 ABIN2987163 ICC IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal
0.0010161072 ABIN2983591 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal
0.0010161072 ABIN2857781 ICC IF WB Rabbit pSer301 Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 37 Antibodies 158 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies 7 Antibodies
Flow Cytometry (FACS) 1 Antibodies 2 Antibodies
Immunochromatography (IC) 4 Antibodies 6 Antibodies
Immunocytochemistry (ICC) 8 Antibodies 25 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies
Immunohistochemistry (IHC) 33 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 21 Antibodies
Immunoprecipitation (IP) 3 Antibodies
Western Blotting (WB) 89 Antibodies
Dot Blot (DB) 22 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Internal Region, pSer345
(56), (47), (37), (31), (28), (19), (14), (13), (12), (10), (9), (6), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(431), (137), (129), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(377), (47), (21), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(354), (173), (125), (55), (51), (34), (22), (20), (18), (8), (6), (4), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Cross-Reactivity Cow (Bovine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
Cross-Reactivity (Details) Rat (100 %), Zebrafish (88 %), Xenopus laevis (88 %), Bovine (100 %)
Characteristics Rabbit Polyclonal antibody to Chk1 (phospho Ser345) (CHK1 checkpoint homolog (S. pombe))
Chk1 (phospho Ser345) antibody [C1C2], Internal
Purification Purified by antigen-affinity chromatography.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Inhibition of CDC25 activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Binds to and phosphorylates RAD51 at 'Thr-309', which may enhance the association of RAD51 with chromatin and promote DNA repair by homologous recombination. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may affect chromatin assembly during S phase or DNA repair. May also phosphorylate multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and enhances suppression of cellular proliferation.

Cellular Localization: Nucleus , Cytoplasm
Molecular Weight 54 kDa
Gene ID 1111
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Western blot1:500-1:3000*
Comment

Positive Control: HCT116 , HCT116 cells with 30uM cisplatin treatment for 24hr

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 1XPBS, 1 % BSA, 20 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857048) ICC/IF Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Se...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857048) IHC-P Image Immunohistochemical analysis of paraffin-embedded HeLa xenograft, using C...
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857048) WB Image Sample: 20 ug of HCT116 P53 whole cell lysate 10% SDS PAGE Chk1-phopho-S345 ...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Internal Region) (ABIN2857048) IHC-P Image Chk1 (phospho Ser345) antibody [C1C2], Internal detects Chk1 (phospho Ser...