anti-Mouse (Murine) CHEK1 antibody for Immunofluorescence

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
pSer301
71
66
39
33
16
16
15
15
14
11
6
5
5
5
5
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
485
195
167
14
14
10
10
10
7
5
4
4
3
2
1
1
1
Host
Rabbit
421
55
23
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
411
177
144
76
68
40
25
21
21
10
7
6
6
5
Options
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Catalog No. ABIN1531555
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
11.177332 ABIN1532571 IF ELISA Rabbit IgG Log in to see Polyclonal 1
11.177332 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal
11.177332 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal
11.177332 ABIN2957528 IC IF WB Rabbit pSer301 Log in to see Polyclonal
11.177332 ABIN1870069 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal
11.177332 ABIN2857048 ICC IF IHC (p) WB Rabbit IgG Internal Region, pSer345 Log in to see Polyclonal
11.177332 ABIN2406850 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal
1 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal
1 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal
1 ABIN1871824 FACS IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN407619 ICC IF IHC (p) IHC WB Rabbit Log in to see Polyclonal
1 ABIN2888805 IF ELISA Rabbit IgG AA 271-320 Log in to see Polyclonal
1 ABIN3197993 IC IF IHC WB Rabbit pSer345 Log in to see Polyclonal
1 ABIN265347 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN2883630 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
1 ABIN2594896 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN2594897 ICC IF IHC (p) WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1977790 IF IHC (p) Rabbit IgG AA 250-300 Log in to see Polyclonal
1 ABIN2882463 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer301
(71), (66), (39), (33), (16), (16), (15), (15), (14), (11), (6), (5), (5), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(485), (195), (167), (14), (14), (10), (10), (10), (7), (5), (4), (4), (3), (2), (1), (1), (1)
Host Rabbit
(421), (55), (23), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(411), (177), (144), (76), (68), (40), (25), (21), (21), (10), (7), (6), (6), (5)
Pubmed 2 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images
Specificity Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Molecular weight of target protein: 54 kDa
Synonyms: CHEK1, Chk1, kinase Chk1
Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

ProductDetails: References for anti-CHEK1 antibody (ABIN1531555)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling ProductDetails: References for anti-CHEK1 antibody (ABIN1531555) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody (ABIN1531555) Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) Antibod...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 1 (CHEK1) (pSer301) antibody (ABIN1531555) Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The ...