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anti-Mouse (Murine) CHEK1 antibody for Western Blotting

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
Middle Region
60
49
36
30
30
15
15
12
10
9
9
6
5
4
4
4
4
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
447
191
163
14
13
9
9
8
7
4
4
3
3
2
1
1
1
Host
Rabbit
386
53
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
377
170
133
71
65
40
22
21
21
10
7
6
6
2
Options
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Catalog No. ABIN2779738
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0923712 ABIN1531555 IF ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal 2
3.0923712 ABIN389542 IHC (p) WB Rabbit Ig pSer280 Log in to see Polyclonal 3
3.0923712 ABIN387806 WB Mouse IgM Log in to see 2G1D5 2
3.0923712 ABIN446614 WB Mouse IgG1 Log in to see 2G1D2 1
3.0923712 ABIN969476 ELISA WB Mouse IgG1 Log in to see 2G1D5 2
3.0923712 ABIN388983 WB Rabbit Ig AA 258-287, Ser280 Log in to see Polyclonal 2
3.0923712 ABIN4297951 IHC IHC (p) WB Rabbit pSer317 Log in to see Polyclonal 3
3.0923712 ABIN1106682 EIA WB Mouse IgG1 Log in to see 2G1D5 1
3.0923712 ABIN2791667 WB Rabbit Middle Region Log in to see Polyclonal
3.0923712 ABIN1531800 ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal
3.0923712 ABIN3043214 WB Rabbit IgG AA 69-87, N-Term Log in to see Polyclonal
3.0923712 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal
3.0923712 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal
3.0923712 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal
3.0923712 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
3.0923712 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Log in to see Polyclonal
3.0923712 ABIN1532831 ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN1532830 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.0923712 ABIN2957528 IC IF WB Rabbit pSer301 Log in to see Polyclonal
3.0923712 ABIN356395 EIA WB Mouse Log in to see 2G1D5

Top referenced anti-CHEK1 antibody for Western Blotting

Similar anti-CHEK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Cow (Bovine) Dog (Canine) Zebrafish Horse (Equine) Rabbit Guinea Pig
Immunofluorescence (fixed cells) (IF/ICC) 10 Antibodies 5 Antibodies 4 Antibodies
Western Blotting (WB) 360 Antibodies 9 Antibodies 13 Antibodies 136 Antibodies 114 Antibodies 3 Antibodies 10 Antibodies 10 Antibodies 5 Antibodies
Proximity Ligation Assay (PLA) 6 Antibodies 3 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Middle Region
(60), (49), (36), (30), (30), (15), (15), (12), (10), (9), (9), (6), (5), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Cow (Bovine), Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus), Zebrafish
(447), (191), (163), (14), (13), (9), (9), (8), (7), (4), (4), (3), (3), (2), (1), (1), (1)
Host Rabbit
(386), (53), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(377), (170), (133), (71), (65), (40), (22), (21), (21), (10), (7), (6), (6), (2)
Pubmed 1 reference available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) Images
Blocking Peptide Blocking peptide for this product available: ABIN974904
Sequence WSCGIVLTAM LAGELPWDQP SDSCQEYSDW KEKKTYLNPW KKIDSAPLAL
Predicted Reactivity Cow: 93%, Dog: 100%, Guinea Pig: 93%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 79%
Characteristics This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Antigen
Alternative Name CHEK1 (CHEK1 Antibody Abstract)
Background CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Alias Symbols: CHK1
Protein Interaction Partner: RELA, CUL4A, UBC, SETMAR, DDB1, TNRC6B, ATR, HECW2, CLSPN, DTL, BTG3, TOPBP1, MAPKAPK2, HSP90AA1, CDC25C, RAD51, PIGM, CUL1, VCP, CDC25A, ATM, APP, MED15, NEK2, TLK1, RB1, BRCA1, FEM1B, XIAP, COPS5, MEPE, LATS2, TP53, Chek1, MEN1, USP7, DNMT1, FANCE, PCNA
Protein Size: 476
Molecular Weight 54 kDa
Gene ID 1111
NCBI Accession NM_001274, NP_001265
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 476 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHEK1 Antibody (ABIN2779738)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/ml
Positive Control...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (Image 2)