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anti-Rat (Rattus) CHEK1 antibody for Enzyme Immunoassay

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Reactivity
Human, Mouse (Murine), Rat (Rattus)
451
149
139
5
4
4
3
2
2
2
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
374
180
138
63
59
34
25
22
20
7
6
5
1
1
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Catalog No. ABIN265347
$ 390.50
Plus shipping costs $45.00

Similar anti-CHEK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 43 Antibodies 41 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 8 Antibodies
Immunofluorescence (IF) 20 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 45 Antibodies 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 92 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(451), (149), (139), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(374), (180), (138), (63), (59), (34), (25), (22), (20), (7), (6), (5), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Specificity This antibody detects endogenous levels of Chk1 protein. (region surrounding asn311)
Cross-Reactivity (Details) Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.
Purification Affinity Chromatography using epitope-specific immunogen.

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name CHK1 (CHEK1 Antibody Abstract)
Background Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by proteolysis of cyclins. Chk1 and Chk2 are involved in these processes as regulators of Cdks. Chk1 and Chk2 both function as essential components in the G2 DNA damage checkpoint by phosphorylating Cdc25C in response to DNA damage. Phosphorylation inhibits Cdc25C activity, thereby blocking mitosis. Cdc25A, Cdc25B and Cdc25C protein tyrosine phosphatases function as mitotic activators by dephosphorylating Cdc2 p34 on regulatory tyrosine residues. It has also been shown that Chk1 can phosphorylate Wee1 in vitro, providing evidence that the hyperphosphorylated form of Wee1, seen in cells delayed by Chk1 overexpression, is due to phosphorylation by Chk1.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1
Molecular Weight approx. 56 kDa
Gene ID 1111
NCBI Accession NP_001107593
UniProt O14757
Research Area Kinases/Phosphatases, Cell Cycle
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes ELISA: 1/10000approx. 1/20000. Western blot: 1/500approx. 1/1000. Immunofuorescence: 1/50-1/200. Immunohistochemistry: 1/50approx. 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
 image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN265347) anti-Checkpoint Kinase 1 (CHEK1) antibody
 image for anti-CHEK1 antibody (Checkpoint Kinase 1) (ABIN265347) anti-Checkpoint Kinase 1 (CHEK1) antibody (Image 2)