You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Rat (Rattus) CHEK1 antibody for ELISA

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
pSer301
56
47
37
32
28
20
14
13
12
9
9
6
4
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
431
137
128
5
4
3
3
2
2
2
1
1
Host
Rabbit
377
47
21
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
12
12
11
8
8
8
3
3
3
3
3
3
3
3
3
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
354
172
125
55
52
34
22
21
18
8
6
4
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1531555
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0012266339 ABIN1532571 IF ELISA Rabbit IgG Log in to see Polyclonal 1
0.0012266339 ABIN1531800 ELISA WB Rabbit IgG pSer317 Log in to see Polyclonal
0.0012266339 ABIN1531801 IHC ELISA Rabbit IgG pSer345 Log in to see Polyclonal
0.0012266339 ABIN1532831 ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012266339 ABIN1532830 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.0012266339 ABIN2163691 IHC ELISA WB Rabbit IgG Ser317 Log in to see Polyclonal
0.0012266339 ABIN2163692 IHC ELISA WB Rabbit IgG Ser317 Log in to see Polyclonal
0.0012266339 ABIN2460510 IHC ELISA WB Rabbit Log in to see Polyclonal
0.0012266339 ABIN2163672 ELISA WB Rabbit IgG Ser345 Log in to see Polyclonal
0.0012266339 ABIN2163671 ELISA WB Rabbit IgG Ser280 Log in to see Polyclonal
0.0012266339 ABIN2163673 IF ELISA WB Rabbit IgG Ser286 Log in to see Polyclonal
0.0012266339 ABIN2163674 IHC ELISA WB Rabbit IgG Ser317 Log in to see Polyclonal
0.0012266339 ABIN2163670 IHC ELISA Rabbit IgG Ser345 Log in to see Polyclonal
0.0012266339 ABIN2163669 IF ELISA Rabbit IgG Log in to see Polyclonal
0.0012266339 ABIN3181961 ELISA IHC WB Rabbit IgG pSer317 Log in to see Polyclonal
0.0012266339 ABIN3181962 ELISA IHC Rabbit IgG pSer345 Log in to see Polyclonal
0.0012266339 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
0.0012266339 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal
0.0012266339 ABIN3183903 ELISA IHC WB Rabbit IgG Ser249 Log in to see Polyclonal
0.0012266339 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 39 Antibodies 37 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 8 Antibodies
Immunofluorescence (IF) 20 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 39 Antibodies 33 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 16 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 83 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer301
(56), (47), (37), (32), (28), (20), (14), (13), (12), (9), (9), (6), (4), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(431), (137), (128), (5), (4), (3), (3), (2), (2), (2), (1), (1)
Host Rabbit
(377), (47), (21), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(12), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(354), (172), (125), (55), (52), (34), (22), (21), (18), (8), (6), (4), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN1531555) Images
Specificity Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN1531555) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Molecular weight of target protein: 54 kDa
Synonyms: CHEK1, Chk1, kinase Chk1
Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN1531555) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN1531555) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CHEK1 Antibody (ABIN1531555)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN1531555) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer301) (ABIN1531555) Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) Antibod...
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer301) (ABIN1531555) Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The ...