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anti-Rat (Rattus) CHEK1 antibody for Immunofluorescence

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
pSer301
58
48
37
34
28
20
14
13
12
9
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
451
149
139
5
4
4
3
2
2
2
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Immunofluorescence (IF), ELISA, Western Blotting (WB)
374
179
138
64
59
34
25
22
20
8
6
5
1
1
Supplier
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Catalog No. ABIN1531555
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN1532571 IF ELISA Rabbit IgG Log in to see Polyclonal 1
0.32040194 ABIN2705868 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.32040194 ABIN2705867 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.32040194 ABIN2163673 IF ELISA WB Rabbit IgG Ser286 Log in to see Polyclonal
0.32040194 ABIN2163669 IF ELISA Rabbit IgG Log in to see Polyclonal
0.32040194 ABIN2406850 IF IHC WB Rabbit IgG pSer280 Log in to see Polyclonal
0.0010840179 ABIN265347 EIA IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN2957528 IC IF WB Rabbit pSer301 Log in to see Polyclonal
0.0010840179 ABIN3183901 ELISA IF IHC WB Rabbit IgG Ser2484 Log in to see Polyclonal
0.0010840179 ABIN3182434 ELISA IF WB Rabbit IgG pSer301 Log in to see Polyclonal
0.0010840179 ABIN3197993 IC IF IHC WB Rabbit pSer345 Log in to see Polyclonal
0.0010840179 ABIN3183900 ELISA IF WB Rabbit IgG Ser2481 Log in to see Polyclonal
0.0010840179 ABIN407619 ICC IF IHC (p) IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN1843354 ICC IF WB Rabbit pSer301 Log in to see Polyclonal
0.0010840179 ABIN1575614 IF ELISA Rabbit IgG Internal Region Log in to see Polyclonal
0.0010840179 ABIN2972901 ICC IF WB Rabbit Center, pSer345 Log in to see Polyclonal
0.0010840179 ABIN2983591 ICC FACS IF WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN1575611 IF ELISA WB Rabbit IgG pSer301 Log in to see Polyclonal
0.0010840179 ABIN2857781 ICC IF WB Rabbit pSer301 Log in to see Polyclonal

Top referenced anti-CHEK1 antibody for Immunofluorescence

Similar anti-CHEK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 43 Antibodies 41 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 8 Antibodies
Immunofluorescence (IF) 20 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 45 Antibodies 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 92 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope pSer301
(58), (48), (37), (34), (28), (20), (14), (13), (12), (9), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(451), (149), (139), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunofluorescence (IF), ELISA, Western Blotting (WB)
(374), (179), (138), (64), (59), (34), (25), (22), (20), (8), (6), (5), (1), (1)
Pubmed 2 references available
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN1531555) Images
Specificity Chk1 (Phospho-Ser301) Antibody detects endogenous levels of Chk1 only when phosphorylated at Ser301.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Chk1 around the phosphorylation site of Ser301.
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN1531555) Images back to top
Antigen
Alternative Name Chk1 (CHEK1 Antibody Abstract)
Background Molecular weight of target protein: 54 kDa
Synonyms: CHEK1, Chk1, kinase Chk1
Molecular Weight 54 kDa
Gene ID 1111
OMIM 603078
UniProt O14757
Research Area Cancer, Cell Cycle, DNA/RNA, Kinases/Phosphatases
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN1531555) Images back to top
Application Notes WB: 1:500-1:1000
IF: 1:100-1:500
ELISA: 1:40000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.24529 (NCBI Gene Symbol: CHK1)

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN1531555) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-CHEK1 Antibody (ABIN1531555)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Matsuoka, Ballif, Smogorzewska, McDonald, Hurov, Luo, Bakalarski, Zhao, Solimini, Lerenthal, Shiloh, Gygi, Elledge: "ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage." in: Science (New York, N.Y.), Vol. 316, Issue 5828, pp. 1160-6, 2007

Sanchez, Wong, Thoma, Richman, Wu, Piwnica-Worms, Elledge: "Conservation of the Chk1 checkpoint pathway in mammals: linkage of DNA damage to Cdk regulation through Cdc25." in: Science (New York, N.Y.), Vol. 277, Issue 5331, pp. 1497-501, 1997

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN1531555) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer301) (ABIN1531555) Western blot analysis of extracts from 293 cells, using Chk1 (Phospho-Ser301) Antibod...
Immunofluorescence (IF) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (pSer301) (ABIN1531555) Immunofluorescence analysis of HeLa cells, using Chk1 (Phospho-Ser301) Antibody. The ...