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anti-Rat (Rattus) CHEK1 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • C85740
  • CHEK1
  • chk1
  • Chk1
  • CHK1
  • id:ibd2720
  • rad27
  • zgc:56093
Epitope
AA 280-330, pSer317
57
48
37
34
28
20
14
12
12
10
9
6
5
4
4
4
4
4
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
451
149
139
5
4
4
3
2
2
2
1
1
Host
Rabbit
393
51
22
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
374
180
138
63
60
33
25
22
20
8
6
5
1
1
Supplier
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Catalog No. ABIN757072
$ 291.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008846439 ABIN875498 IF (p) Alexa Fluor 488 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN875500 IF (p) Alexa Fluor 647 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN875501 IF (p) Alexa Fluor 350 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN875499 IF (p) Alexa Fluor 555 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN757075 IF (p) Cy3 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN757076 IF (p) Cy5 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN757078 IF (p) Cy7 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN757079 IF (p) FITC Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN757077 IF (p) Cy5.5 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN2811603 IF (p) Alexa Fluor 594 Rabbit IgG AA 280-330, pSer317 Log in to see Polyclonal
0.0008846439 ABIN875673 IF (p) Alexa Fluor 488 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN875675 IF (p) Alexa Fluor 555 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN875671 IF (p) Alexa Fluor 647 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN875674 IF (p) Alexa Fluor 350 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN757483 IF (p) Cy7 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN757484 IF (p) FITC Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN757480 IF (p) Cy3 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN757481 IF (p) Cy5 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN757482 IF (p) Cy5.5 Rabbit IgG pSer345 Log in to see Polyclonal
0.0008846439 ABIN2811604 IF (p) Alexa Fluor 594 Rabbit IgG pSer345 Log in to see Polyclonal

Similar anti-CHEK1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 43 Antibodies 41 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 4 Antibodies
Flow Cytometry (FACS) 1 Antibodies 1 Antibodies
Immunochromatography (IC) 4 Antibodies 4 Antibodies
Immunocytochemistry (ICC) 5 Antibodies 8 Antibodies
Immunofluorescence (IF) 20 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 23 Antibodies 23 Antibodies
Immunohistochemistry (IHC) 45 Antibodies 40 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 22 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 92 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope AA 280-330, pSer317
(57), (48), (37), (34), (28), (20), (14), (12), (12), (10), (9), (6), (5), (4), (4), (4), (4), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(451), (149), (139), (5), (4), (4), (3), (2), (2), (2), (1), (1)
Host Rabbit
(393), (51), (22), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(374), (180), (138), (63), (60), (33), (25), (22), (20), (8), (6), (5), (1), (1)
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Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling Images
Specificity This phosphorylation site is homologous to that of Ser317 in Mouse and Rat.
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human CHEK1 around the phosphorylation site of Ser317
Isotype IgG

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name Chek1 (CHEK1 Antibody Abstract)
Background

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest and activation of DNA repair in response to the presence of DNA damage or unreplicated DNA. May also negatively regulate cell cycle progression during unperturbed cell cycles. This regulation is achieved by a number of mechanisms that together help to preserve the integrity of the genome. Recognizes the substrate consensus sequence [R-X-X-S/T]. Binds to and phosphorylates CDC25A, CDC25B and CDC25C. Phosphorylation of CDC25A at 'Ser-178' and 'Thr-507' and phosphorylation of CDC25C at 'Ser-216' creates binding sites for 14-3-3 proteins which inhibit CDC25A and CDC25C. Phosphorylation of CDC25A at 'Ser-76', 'Ser-124', 'Ser-178', 'Ser-279' and 'Ser-293' promotes proteolysis of CDC25A. Phosphorylation of CDC25A at 'Ser-76' primes the protein for subsequent phosphorylation at 'Ser-79', 'Ser-82' and 'Ser-88' by NEK11, which is required for polyubiquitination and degradation of CDCD25A. Inhibition of CDC25 leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. Also phosphorylates NEK6. Binds to and phosphorylates RAD51 at 'Thr-309', which promotes the release of RAD51 from BRCA2 and enhances the association of RAD51 with chromatin, thereby promoting DNA repair by homologous recombination. Phosphorylates multiple sites within the C-terminus of TP53, which promotes activation of TP53 by acetylation and promotes cell cycle arrest and suppression of cellular proliferation. Also promotes repair of DNA cross-links through phosphorylation of FANCE. Binds to and phosphorylates TLK1 at 'Ser-743', which prevents the TLK1-dependent phosphorylation of the chromatin assembly factor ASF1A. This may enhance chromatin assembly both in the presence or absence of DNA damage. May also play a role in replication fork maintenance through regulation of PCNA.

Subcellular location: Cytoplasm, Nucleus


Synonyms: CHK1, Serine/threonine-protein kinase Chk1, CHK1 checkpoint homolog, Cell cycle checkpoint kinase, Checkpoint kinase-1, CHEK1
Gene ID 1111
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 100 μg/mL BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 280-330) (ABIN757072) Formalin-fixed and paraffin embedded mouse small intestine tissue labeled with Anti-p...
SDS-PAGE (SDS) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (AA 280-330) (ABIN757072) Lane 1: rat brain lysates Lane 2: rat heart lysates probed with Anti phospho-CHEK1(Se...