You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Xenopus laevis CHEK1 antibody for Immunohistochemistry

Recommended CHEK1 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 1 (CHEK1) Antibodies
  • An08g10320
  • AO090003000441
  • CHK1
  • chk1
  • C85740
  • Chk1
  • rad27
  • id:ibd2720
  • zgc:56093
  • CHEK1
  • checkpoint kinase 1
  • serine/threonine-protein kinase chk1
  • serine/threonine-protein kinase Chk1
  • CHK1 checkpoint homolog (S. pombe)
  • STT3, subunit of the oligosaccharyltransferase complex, homolog A (S. cerevisiae)
  • CHEK1
  • ANI_1_2436074
  • AOR_1_768154
  • PTRG_04183
  • SJAG_01680
  • PAAG_04978
  • MCYG_03290
  • VDBG_03742
  • chek1
  • Chek1
  • STT3A
Epitope
Middle Region
62
50
36
34
30
15
15
13
12
10
9
6
5
5
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
469
199
173
14
12
8
8
8
7
4
4
3
3
3
1
1
1
1
Host
Rabbit
404
56
24
6
Clonality
Polyclonal
Conjugate
This CHEK1 antibody is un-conjugated
13
12
11
8
8
8
3
3
3
3
3
3
3
3
3
3
3
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
396
185
143
71
67
40
23
22
22
9
7
7
6
2
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN2779738
$ 289.00
Plus shipping costs $45.00

Top referenced anti-CHEK1 antibody for Immunohistochemistry

Similar anti-CHEK1 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Chicken Cow (Bovine) Dog (Canine) Xenopus laevis Zebrafish (Danio rerio)
Immunofluorescence (fixed cells) (IF/ICC) 9 Antibodies 5 Antibodies 4 Antibodies
Western Blotting (WB) 378 Antibodies 4 Antibodies 8 Antibodies 12 Antibodies 141 Antibodies 121 Antibodies 2 Antibodies 1 Antibodies
Proximity Ligation Assay (PLA) 7 Antibodies 4 Antibodies 1 Antibodies 1 Antibodies
Immunoprecipitation (IP) 19 Antibodies 6 Antibodies 4 Antibodies 2 Antibodies

General

Antigen Checkpoint Kinase 1 (CHEK1) Antibodies
Epitope Middle Region
(62), (50), (36), (34), (30), (15), (15), (13), (12), (10), (9), (6), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Rat (Rattus), Xenopus laevis, Zebrafish (Danio rerio)
(469), (199), (173), (14), (12), (8), (8), (8), (7), (4), (4), (3), (3), (3), (1), (1), (1), (1)
Host Rabbit
(404), (56), (24), (6)
Clonality
Polyclonal
Conjugate This CHEK1 antibody is un-conjugated
(13), (12), (11), (8), (8), (8), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(396), (185), (143), (71), (67), (40), (23), (22), (22), (9), (7), (7), (6), (2)
Pubmed 1 reference available
Supplier Log in to see

Product Details anti-CHEK1 Antibody

Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) Images
Blocking Peptide Blocking peptide for this product available: ABIN974904
Sequence WSCGIVLTAM LAGELPWDQP SDSCQEYSDW KEKKTYLNPW KKIDSAPLAL
Predicted Reactivity Dog : 100 %, Horse : 100 %, Human : 100 %, Mouse : 100 %, Bovine : 90 %, Chicken : 90 %, Guinea pig : 90 %, Pig : 90 %, Zebrafish : 70 %
Characteristics This is a rabbit polyclonal antibody against CHEK1. It was validated on Western Blot and immunohistochemistry.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the middle region of human CHEK1

Target Details CHEK1

Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Antigen
Alternative Name CHEK1 (CHEK1 Antibody Abstract)
Background CHEK1 is required during normal S phase to avoid aberrantly increased initiation of DNA replication, thereby protecting against DNA breakage. Its expression is dispensable for somatic cell death and critical for sustaining G2 DNA damage checkpoint.
Molecular Weight 54 kDa
Gene ID 1111
NCBI Accession NM_001274, NP_001265
UniProt O14757
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 476 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN2779738) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-CHEK1 Antibody (ABIN2779738)

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Background publications

Krämer, Mailand, Lukas, Syljuåsen, Wilkinson, Nigg, Bartek, Lukas: "Centrosome-associated Chk1 prevents premature activation of cyclin-B-Cdk1 kinase." in: Nature cell biology, Vol. 6, Issue 9, pp. 884-91, 2004

Images

Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN2779738) back to top
Supplier Images
Western Blotting (WB) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) WB Suggested Anti-CHEK1 Antibody Titration: 0.2-1 ug/ml
Positive Control...
Immunohistochemistry (IHC) image for anti-CHEK1 antibody (Checkpoint Kinase 1) (Middle Region) (ABIN2779738) anti-Checkpoint Kinase 1 (CHEK1) (Middle Region) antibody (Image 2)