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|Antigen||Checkpoint Kinase 1 (CHEK1) Antibodies|
|Epitope||AA 312-327 Alternatives|
|Conjugate||This CHEK1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
|Supplier||Log in to see|
Product Details anti-CHEK1 AntibodyTarget Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN401324) Images
|Specificity||This antibody is directed against yeast CHK1 protein.|
|Immunogen||Synthetic peptide corresponding aa 312-327 of S. cerevisiae CHK1|
Target Details CHEK1Product Details anti-CHEK1 Antibody Application Details Handling References for anti-CHEK1 Antibody (ABIN401324) Images back to top
|Alternative Name||CHK1 (CHEK1 Antibody Abstract)|
|Background||CHK1 (also known as serine/threonine-protein kinase CHK1 and checkpoint kinase 1) is involved in cell cycle arrest when DNA damage has occurred or when unligated DNA is present. The kinase controls phosphorylation and abundance of PDS1 to prevent anaphase entry. Also helps prevent mitotic exit. CHK1 is localized within the nucleus.Synonyms: CHEK-1, CHEK1, CHK1 checkpoint homolog, Serine/threonine-protein kinase Chk1|
|Research Area||Kinases/Phosphatases, Cell Cycle|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
Application DetailsProduct Details anti-CHEK1 Antibody Target Details CHEK1 Handling References for anti-CHEK1 Antibody (ABIN401324) Images back to top
ELISA: 1/2,000 - 1/10,000. Western Blot: 1/200 - 1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details References for anti-CHEK1 Antibody (ABIN401324) Images back to top
|Concentration||0.73 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-CHEK1 Antibody (ABIN401324)Product Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling Images back to top
Sanchez, Bachant, Wang et al.: "Control of the DNA damage checkpoint by chk1 and rad53 protein kinases through distinct mechanisms." in: Science (New York, N.Y.), Vol. 286, Issue 5442, pp. 1166-71, 1999 (PubMed).
ImagesProduct Details anti-CHEK1 Antibody Target Details CHEK1 Application Details Handling References for anti-CHEK1 Antibody (ABIN401324) back to top