anti-Human CHEK2 antibody for Enzyme Immunoassay

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • LOC396525
Epitope
AA 64-73, pThr68
51
51
39
36
19
17
15
15
15
13
12
10
8
8
6
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
487
218
198
19
18
16
13
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
352
136
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
9
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
399
126
124
107
90
66
48
43
27
6
6
4
2
1
1
Supplier
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Catalog No. ABIN318831
$ 544.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN5556683 EIA WB Rabbit AA 64-73, pThr68 Log in to see Polyclonal
1 ABIN5554688 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN1106684 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1C12B8 3
1 ABIN2718529 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 64-73, pThr68
(51), (51), (39), (36), (19), (17), (15), (15), (15), (13), (12), (10), (8), (8), (6), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(487), (218), (198), (19), (18), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(352), (136), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(10), (9), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(399), (126), (124), (107), (90), (66), (48), (43), (27), (6), (6), (4), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN318831) Images
Specificity This antibody detects Checkpoint kinase 2 (Chk2), phosphorylated at Threonine 68 (Thr68).
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.
Purification Purified
Immunogen Synthetic peptide corresponding to amino acids 64-73 of human Chk2.
Isotype IgG

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN318831) Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Chk2 is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radiation, and phsophorylate Thr68 and other sites in the amino-terminal region of Chk2, leading to Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, preventing the entry into mitosis, and has been shown to stabilize the tumour suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumour suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familial cancer usually associated with inherited mutations in TP53.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
NCBI Accession NP_001005735
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling ProductDetails: References for anti-CHEK2 antibody (ABIN318831) Images back to top
Application Notes ELISA: 1: 10000 - 1: 50000. Western Blot: 1: 200 - 1: 2000, detects a band of approximately 61 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details ProductDetails: References for anti-CHEK2 antibody (ABIN318831) Images back to top
Concentration 1.0mg/mL
Buffer PBS, pH 7.2, 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

ProductDetails: References for anti-CHEK2 antibody (ABIN318831)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Background publications

Bartek, Lukas: "Chk1 and Chk2 kinases in checkpoint control and cancer." in: Cancer cell, Vol. 3, Issue 5, pp. 421-9, 2003