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anti-Human Checkpoint Kinase 2 antibody for Flow Cytometry

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Epitope
AA 111-141, N-Term
44
41
38
32
24
15
13
13
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
408
161
160
10
8
7
7
7
5
1
1
1
Host
Rabbit
323
91
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
326
120
117
63
62
56
37
26
23
6
5
2
1
Supplier
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Catalog No. ABIN1881196
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0010476487 ABIN1497495 FACS IF IHC (p) WB Mouse IgG1 Log in to see 10G7
0.0010476487 ABIN1497498 FACS IHC (p) IP WB Mouse IgG2b Log in to see 4C10
0.0010476487 ABIN1497499 FACS IHC (p) IP WB Mouse IgG1 Log in to see 5C7
0.0010476487 ABIN1497497 FACS IHC (p) IP WB Mouse IgG2b Log in to see 2H5
0.0010476487 ABIN1497500 FACS IHC (p) WB Mouse IgG1 Log in to see 8D11
0.0010476487 ABIN2452427 FACS IF IP IHC WB Mouse IgG1 Log in to see 5C4
0.0010476487 ABIN2452258 FACS IF WB Mouse IgG2b Log in to see 2F4
0.0010476487 ABIN2452501 FACS IP IHC WB Mouse IgG1 Log in to see 5C7
0.0010476487 ABIN2452498 FACS IP IHC WB Mouse IgG2b Log in to see 4C10
0.0010476487 ABIN2452507 FACS IP IHC WB Mouse IgG2b Log in to see 2H5
0.0010476487 ABIN2452500 FACS IF IHC WB Mouse IgG1 Log in to see 10G7
0.0010476487 ABIN2452424 FACS IHC WB Mouse IgG1 Log in to see 8D11
0.0010476487 ABIN1497496 FACS IF IHC (p) IP WB Mouse IgG1 Log in to see 5C4
0.0010476487 ABIN219984 ICC FACS IF IP ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
0.0010476487 ABIN2232881 IC FACS IF IP WB Rabbit IgG pThr68 Log in to see Polyclonal
0.0010476487 ABIN2137536 FACS IHC WB Mouse IgG1 Log in to see 8D11
0.0010476487 ABIN2137535 FACS IF IHC WB Mouse IgG1 Log in to see 5C4
0.0010476487 ABIN2137539 FACS IHC WB Mouse IgG2b Log in to see 2H5
0.0010476487 ABIN2137540 FACS IHC WB Mouse IgG2b Log in to see 4C10
0.0010476487 ABIN2137538 FACS IHC WB Mouse IgG1 Log in to see 5C7

Top referenced anti-Checkpoint Kinase 2 antibody for Flow Cytometry

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human
ELISA 109 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 24 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 64 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 117 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies
Immunoprecipitation (IP) 36 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 316 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 111-141, N-Term
(44), (41), (38), (32), (24), (15), (13), (13), (12), (11), (10), (9), (9), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(408), (161), (160), (10), (8), (7), (7), (7), (5), (1), (1), (1)
Host Rabbit
(323), (91), (8), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate Un-conjugated
(8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(326), (120), (117), (63), (62), (56), (37), (26), (23), (6), (5), (2), (1)
Pubmed 3 references available
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Product details

Target details Application Details Handling References Images
Purification This antibody is purified through a protein A column, followed by peptide affinity purification. Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Immunogen This CHEK2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 111-141 amino acids from the N-terminal region of human CHEK2.
Clone RB19067

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53.

Synonyms: CHEK2, CDS1, CHK2, RAD53, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Checkpoint kinase 2
NCBI Accession NP_009125, NP_665861, NP_001244316, NP_001005735
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling References Images back to top
Application Notes WB: 1:1000,IHC (p): 1:50-100,FACS: 1:10-50,IF: 1:10-50
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.
Preservative Sodium azide
Precaution of Use WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

References

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Product cited in:

Yang, Wood, Hrushesky: "Mammalian TIMELESS is required for ATM-dependent CHK2 activation and G2/M checkpoint control." in: The Journal of biological chemistry, Vol. 285, Issue 5, pp. 3030-4, 2010

Varmark, Kwak, Theurkauf: "A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells." in: Cell cycle (Georgetown, Tex.), Vol. 9, Issue 2, pp. 312-20, 2010

Zhu, Miao, Huang, Feng, Zhang, Lu, Cai, Tong, Xu, Qian, Ding: "Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells." in: Neoplasia (New York, N.Y.), Vol. 11, Issue 11, pp. 1226-34, 2009

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Flow Cytometry (FACS) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 111-141), (N-Term) antibody (ABIN1881196) CHEK2 Antibody (N-term) (ABIN1881196) flow cytometric analysis of K562 cells (right h...