anti-Human CHEK2 antibody for Immunocytochemistry

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • OPN2SW
Epitope
AA 481-531
51
50
39
35
19
17
15
15
15
13
12
10
7
7
7
5
4
3
3
3
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
481
215
195
19
19
16
13
8
5
4
2
1
1
1
1
1
1
Host
Mouse
346
136
8
1
1
Clonality
Monoclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
9
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
395
124
119
108
91
66
47
43
27
6
6
5
2
1
1
Options
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Catalog No. ABIN1724714
$ 466.40
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN151943 ICC IF WB Mouse IgG Log in to see 8F12 2
1 ABIN213921 ICC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4297968 FACS ICC IF IHC IHC (p) IP WB Mouse IgG1 Transcript Variant 1 Log in to see 5C4
1 ABIN1871829 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal
1 ABIN4297976 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN5631977 ELISA ICC IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN4297967 ELISA ICC IF IHC IHC (fro) IHC (p) WB Mouse IgG2b Log in to see 1C12B8
1 ABIN4297971 ELISA ICC IF WB Rabbit Log in to see Polyclonal
1 ABIN2869319 ELISA ICC IHC WB Mouse IgG2b AA 481-531 Log in to see 1C12B8
1 ABIN4297972 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal
1 ABIN210859 ICC IP WB Mouse IgG1 Log in to see
1 ABIN5631979 ICC IF WB DyLight 550 Mouse IgG Log in to see 8F12
1 ABIN5631981 ICC IF FITC Mouse IgG Log in to see 8F12
1 ABIN5631978 ICC IF WB Biotin Mouse IgG Log in to see 8F12
1 ABIN5631980 ICC IF WB DyLight 650 Mouse IgG Log in to see 8F12
1 ABIN219984 ICC FACS IF IP ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
1 ABIN4263056 ICC IF WB PerCP Mouse IgG Log in to see 8F12
1 ABIN4263059 ICC IF WB DyLight 488 Mouse IgG Log in to see 8F12
1 ABIN4263076 ICC IF WB Alexa Fluor 488 Mouse IgG Log in to see 8F12
1 ABIN4263058 ICC IF WB DyLight 755 Mouse IgG Log in to see 8F12

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 481-531
(51), (50), (39), (35), (19), (17), (15), (15), (15), (13), (12), (10), (7), (7), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(481), (215), (195), (19), (19), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(346), (136), (8), (1), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This CHEK2 antibody is un-conjugated
(10), (9), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Immunocytochemistry (ICC), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(395), (124), (119), (108), (91), (66), (47), (43), (27), (6), (6), (5), (2), (1), (1)
Pubmed 3 references available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN1724714) Images
Immunogen Purified recombinant fragment of human CHK2 (aa481-531) expressed in E. Coli.
Clone 1C12B8
Isotype IgG2b

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling References for anti-CHEK2 antibody (ABIN1724714) Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background CHK2: CHK2 checkpoint homolog (S. pombe). In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 61 kDa
Gene ID 11200
HGNC 11200
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling References for anti-CHEK2 antibody (ABIN1724714) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, ICC: 1:200 - 1:1000
Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details References for anti-CHEK2 antibody (ABIN1724714) Images back to top
Format Liquid
Buffer Ascitic fluid containing 0.03 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

References for anti-CHEK2 antibody (ABIN1724714)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Product cited in:

Cybulski, Masojc, Oszutowska, Jaworowska, Grodzki, Waloszczyk, Serwatowski, Pankowski, Huzarski, Byrski, Górski, Jakubowska, Debniak, Wokolorczyk, Gronwald, Tarnowska, Serrano-Fernández, Lubinski et al.: "Constitutional CHEK2 mutations are associated with a decreased risk of lung and laryngeal cancers. ..." in: Carcinogenesis, Vol. 29, Issue 4, pp. 762-5, 2008

Antoni, Sodha, Collins, Garrett: "CHK2 kinase: cancer susceptibility and cancer therapy - two sides of the same coin?" in: Nature reviews. Cancer, Vol. 7, Issue 12, pp. 925-36, 2007

Bell, Kim, Godwin, Schiripo, Harris, Haserlat, Wahrer, Haiman, Daly, Niendorf, Smith, Sgroi, Garber, Olopade, Le Marchand, Henderson, Altshuler, Haber, Freedman: "Genetic and functional analysis of CHEK2 (CHK2) variants in multiethnic cohorts." in: International journal of cancer. Journal international du cancer, Vol. 121, Issue 12, pp. 2661-7, 2007

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling References for anti-CHEK2 antibody (ABIN1724714) back to top
Supplier Images
Immunocytochemistry (ICC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 481-531) antibody (ABIN1724714) anti-Checkpoint Kinase 2 (CHEK2) (AA 481-531) antibody
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 481-531) antibody (ABIN1724714) anti-Checkpoint Kinase 2 (CHEK2) (AA 481-531) antibody (Image 2)
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 481-531) antibody (ABIN1724714) anti-Checkpoint Kinase 2 (CHEK2) (AA 481-531) antibody (Image 3)