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anti-Human Checkpoint Kinase 2 antibody for Immunofluorescence

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Epitope
N-Term
44
43
39
32
24
15
13
13
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
456
185
166
10
9
8
8
7
5
1
1
1
Host
Rabbit
333
129
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
374
128
124
82
76
56
46
40
25
6
4
2
1
1
Supplier
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Catalog No. ABIN499619
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.32040194 ABIN1881196 FACS IF IHC (p) WB Rabbit AA 111-141, N-Term Log in to see Polyclonal 3
0.32040194 ABIN1531498 IF ELISA WB Rabbit IgG pThr383 Log in to see Polyclonal
0.32040194 ABIN1106684 EIA IF IHC (p) WB Mouse IgG2b Log in to see 1C12B8 3
0.32040194 ABIN2452258 FACS IF WB Mouse IgG2b Log in to see 2F4
0.32040194 ABIN2707424 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
0.32040194 ABIN2163677 IF ELISA WB Rabbit IgG Thr383 Log in to see Polyclonal
0.32040194 ABIN932519 IF IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8
0.32040194 ABIN524629 IF WB Mouse AA 1-543, full length Log in to see Polyclonal
0.32040194 ABIN564867 IF IHC (p) ELISA WB Mouse IgG2a kappa AA 1-543, full length Log in to see 4B7
0.32040194 ABIN2472955 IF IHC (p) WB Mouse IgG2a AA 1-544 Log in to see 4B7
0.32040194 ABIN524630 IF WB Mouse AA 1-543, full length Log in to see Polyclonal
0.32040194 ABIN2405164 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0010840179 ABIN968596 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
0.0010840179 ABIN968597 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
0.0010840179 ABIN393759 IF WB Mouse IgG2a kappa AA 1-544 Log in to see 4B7 5
0.0010840179 ABIN271879 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0010840179 ABIN302804 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0010840179 ABIN223048 IF WB Rabbit IgG N-Term Log in to see Polyclonal
0.0010840179 ABIN466738 IF IHC ELISA WB Mouse IgG2b Log in to see 1C12B8
0.0010840179 ABIN1497495 FACS IF IHC (p) WB Mouse IgG1 Log in to see 10G7

Top referenced anti-Checkpoint Kinase 2 antibody for Immunofluorescence

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 45 Antibodies 45 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies 15 Antibodies
Immunofluorescence (IF) 23 Antibodies 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 51 Antibodies 54 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies 31 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 115 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope N-Term
(44), (43), (39), (32), (24), (15), (13), (13), (12), (11), (10), (9), (9), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(456), (185), (166), (10), (9), (8), (8), (7), (5), (1), (1), (1)
Host Rabbit
(333), (129), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(374), (128), (124), (82), (76), (56), (46), (40), (25), (6), (4), (2), (1), (1)
Pubmed 4 references available
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Product details

Target details Application Details Handling References Images
Specificity Chk2 antibody was raised against a synthetic peptide corresponding to amino acids near the amino terminus of human Chk2.
Purification Affinity chromatography purified via peptide column
Immunogen Human Chk2 (N-Terminus) Peptide
Isotype IgG

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including Chk2 , p53R2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. The checkpoint kinase Chk2 is the mammalian homologue of yeast Cds1/Rad53. In response to DNA damage, the checkpoint kinase ATM phosphorylates and activates Chk2, which in turn directly phosphorylates and activates p53 (7,8). Chk2 serves as ATM downstream effector to mediate activation of p53. Chk2 also phosphorylates and activates BRCA1, the product of a tumor suppressor gene that is mutated in breast and ovarian cancer (9).Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
NCBI Accession NP_009125
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling References Images back to top
Application Notes ELISA. Western Blot: Chk2 antibody can be used for detection of Chk2 at 0.5 to 1 μg/mL. A 60 kDaband can be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Concentration 1 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.

References

Product details Target details Application Details Handling Images back to top
Background publications

Tanaka, Arakawa, Yamaguchi, Shiraishi, Fukuda, Matsui, Takei, Nakamura: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000

Oda, Ohki, Murasawa, Nemoto, Shibue, Yamashita, Tokino, Taniguchi, Tanaka: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000

Oda, Arakawa, Tanaka, Matsuda, Tanikawa, Mori, Nishimori, Tamai, Tokino, Nakamura, Taya: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Immunofluorescence (IF) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN499619) Immunocytochemistry of Chk2 in Jurkat cells with AP30226PU-N Chk2 antibody at 1 μg/ml.
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN499619) Western blot analysis of Chk2 expression in K562 (A), Jurkat (B), and HL-60 (C) whole...