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anti-Human Checkpoint Kinase 2 antibody for Immunohistochemistry

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CHK-2
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • MCYG_07308
  • LOC396525
  • CHEK2
  • Chek2
  • chek2
Epitope
N-Term
48
47
36
34
19
17
15
15
14
13
12
10
8
8
6
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
458
217
198
19
19
16
14
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
329
131
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
8
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (IHC), Western Blotting (WB)
374
119
116
99
83
66
44
42
26
6
6
5
2
1
1
Options
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Catalog No. ABIN2779312
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.238905 ABIN1724714 ICC IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 3
9.238905 ABIN1531803 IHC ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
9.238905 ABIN1531263 IHC ELISA WB Rabbit IgG pThr387 Log in to see Polyclonal
9.238905 ABIN1532281 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.238905 ABIN1532833 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
9.238905 ABIN2452424 FACS IHC WB Mouse IgG1 Log in to see 8D11
9.238905 ABIN2452498 FACS IP IHC WB Mouse IgG2b Log in to see 4C10
9.238905 ABIN2452500 FACS IF IHC WB Mouse IgG1 Log in to see 10G7
9.238905 ABIN2452501 FACS IP IHC WB Mouse IgG1 Log in to see 5C7
9.238905 ABIN2452507 FACS IP IHC WB Mouse IgG2b Log in to see 2H5
9.238905 ABIN2452427 FACS IF IP IHC WB Mouse IgG1 Log in to see 5C4
9.238905 ABIN2705870 IHC WB Rabbit pSer387 Log in to see Polyclonal
9.238905 ABIN2707424 IC IF IHC WB Rabbit N-Term Log in to see Polyclonal
9.238905 ABIN1871829 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal
9.238905 ABIN3023035 IHC WB Rabbit IgG Log in to see Polyclonal
9.238905 ABIN932519 IF IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8
9.238905 ABIN4297963 IHC IHC (p) WB Rabbit Log in to see Polyclonal
9.238905 ABIN2405164 ICC IF IP IHC WB Rabbit IgG Log in to see Polyclonal
9.238905 ABIN1843154 ELISA IF/ICC IHC WB Mouse IgG2b AA 481-531 Log in to see
9.238905 ABIN1848092 IHC ELISA WB Rabbit N-Term, Thr68 Log in to see Polyclonal

Top referenced anti-Checkpoint Kinase 2 antibody for Immunohistochemistry

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human
ELISA 107 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 42 Antibodies
Immunofluorescence (IF) 83 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 119 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 98 Antibodies
Immunoprecipitation (IP) 43 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 366 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope N-Term
(48), (47), (36), (34), (19), (17), (15), (15), (14), (13), (12), (10), (8), (8), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(458), (217), (198), (19), (19), (16), (14), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(329), (131), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(9), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (IHC), Western Blotting (WB)
(374), (119), (116), (99), (83), (66), (44), (42), (26), (6), (6), (5), (2), (1), (1)
Pubmed 3 references available
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Product details

Target details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN974905
Sequence MSRESDVEAQ QSHGSSACSQ PHGSVTQSQG SSSQSQGISS SSTSTMPNSS
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against CHEK2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2

Target details

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Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, CHEK2 is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in its gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in its gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: RP11-436C9.1, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Protein Interaction Partner: SRPK1, PPP2CA, PLK1, CHEK2, PRKDC, ATM, POLR2L, RCHY1, VHL, UBC, NR4A1, ELAVL1, CDC25A, ARHGAP1, PER3, CDC25C, CUL1, CUL4A, KPNA2, APP, STRAP, TP53, BRCA1, RB1, DAPK3, UBR5, PML, MDM2, BRCA2, KAT2B, LATS2, PSME3, TRIM28, RNF8, STAT1, PPP2R5E, PPP2R5D, PPP
Protein Size: 514
Molecular Weight 58 kDa
Gene ID 11200
NCBI Accession NM_145862, NP_665861
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

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Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 514 AA

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Kobayashi, Lin: "Antiangiogenic and radiotherapy for cancer treatment." in: Histology and histopathology, Vol. 21, Issue 10, pp. 1125-34, 2006

Ribeiro-Silva, Moutinho, Moura, Vale, Zucoloto: "Expression of checkpoint kinase 2 in breast carcinomas: correlation with key regulators of tumor cell proliferation, angiogenesis, and survival." in: Histology and histopathology, Vol. 21, Issue 4, pp. 373-82, 2006

Product cited in:

Yomo, Hongo, Kuroyanagi, Kobayashi: "Parkinsonism and midbrain dysfunction after shunt placement for obstructive hydrocephalus." in: Journal of clinical neuroscience : official journal of the Neurosurgical Society of Australasia, Vol. 13, Issue 3, pp. 373-8, 2006

Images

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Supplier Images
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN2779312) Immunohistochemistry with Placenta tissue at an antibody concentration of 10µg/ml usi...