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anti-Human Checkpoint Kinase 2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Epitope
AA 465-498, C-Term
44
43
40
32
23
15
13
13
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
456
185
166
10
9
8
8
7
5
1
1
1
Host
Rabbit
333
129
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
374
128
124
83
75
56
46
40
25
6
5
2
1
1
Supplier
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Catalog No. ABIN3043811
$ 240.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.34288716 ABIN498829 IHC (p) Rabbit pThr387 Log in to see Polyclonal
0.34288716 ABIN213921 ICC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.34288716 ABIN498830 IHC (p) Rabbit pThr68 Log in to see Polyclonal
0.34288716 ABIN213892 IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1
0.34288716 ABIN498917 IHC (p) Rabbit Log in to see Polyclonal
0.34288716 ABIN498918 IHC (p) Rabbit Log in to see Polyclonal
0.0008982679 ABIN487312 IHC (p) IP WB Mouse IgG2a Log in to see DCS-270 4
0.0008982679 ABIN302248 IHC (p) WB Mouse IgG1 Log in to see 73C175-1-1 6
0.0008982679 ABIN272011 IHC (p) WB Rabbit pSer379 Log in to see Polyclonal
0.0008982679 ABIN271879 IF IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN265350 IHC (p) WB Rabbit Log in to see Polyclonal
0.0008982679 ABIN1881196 FACS IF IHC (p) WB Rabbit AA 111-141, N-Term Log in to see Polyclonal 3
0.0008982679 ABIN302804 IF IHC (p) WB Rabbit N-Term Log in to see Polyclonal
0.0008982679 ABIN487488 IHC (p) IP WB Mouse IgG2a Log in to see DCS-273
0.0008982679 ABIN2878986 IHC (p) ELISA WB Rabbit IgG AA 35-84 Log in to see Polyclonal
0.0008982679 ABIN317991 IHC (p) WB Rabbit pThr68 Log in to see Polyclonal
0.0008982679 ABIN1497498 FACS IHC (p) IP WB Mouse IgG2b Log in to see 4C10
0.0008982679 ABIN1497497 FACS IHC (p) IP WB Mouse IgG2b Log in to see 2H5
0.0008982679 ABIN1497495 FACS IF IHC (p) WB Mouse IgG1 Log in to see 10G7
0.0008982679 ABIN1497500 FACS IHC (p) WB Mouse IgG1 Log in to see 8D11

Top referenced anti-Checkpoint Kinase 2 antibody for Immunohistochemistry (Paraffin-embedded Sections)

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Western Blotting (WB) 362 Antibodies 135 Antibodies 115 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Immunoprecipitation (IP) 39 Antibodies 6 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies 31 Antibodies 27 Antibodies
Immunohistochemistry (IHC) 127 Antibodies 54 Antibodies 51 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies 44 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 465-498, C-Term
(44), (43), (40), (32), (23), (15), (13), (13), (12), (11), (10), (9), (9), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(456), (185), (166), (10), (9), (8), (8), (7), (5), (1), (1), (1)
Host Rabbit
(333), (129), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(374), (128), (124), (83), (75), (56), (46), (40), (25), (6), (5), (2), (1), (1)
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Product details

Target details Application Details Handling Images
Sequence KLLVVDPKAR FTTEEALRHP WLQDEDMKRK FQDL
Cross-Reactivity (Details) No cross reactivity with other proteins.
Characteristics Rabbit IgG polyclonal antibody for Serine/threonine-protein kinase Chk2(CHEK2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: checkpoint kinase 2
Protein Name: Serine/threonine-protein kinase Chk2
Purification Immunogen affinity purified.
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human Chk2 (465-498aa KLLVVDPKARFTTEEALRHPWLQDEDMKRKFQDL), different from the related mouse sequence by four amino acids.
Isotype IgG

Target details

Product details Application Details Handling Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest. Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs. It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA1. Additionally, CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment. Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Moreover, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast. There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.
Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

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Application Notes WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.
Comment

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Restrictions For Research Use only

Handling

Product details Target details Application Details Images back to top
Format Lyophilized
Reconstitution Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
Concentration 500 μg/mL
Buffer Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C,4 °C
Storage Comment At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Images

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Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 465-498), (C-Term) antibody (ABIN3043811) anti-Checkpoint Kinase 2 (CHEK2) (AA 465-498), (C-Term) antibody
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 465-498), (C-Term) antibody (ABIN3043811) anti-Checkpoint Kinase 2 (CHEK2) (AA 465-498), (C-Term) antibody (Image 2)