anti-Human CHEK2 antibody for Western Blotting

Recommended CHEK2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • CHK-2
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • CHEK2
  • chek2
  • Chek2
  • MCYG_07308
  • LOC396525
Epitope
N-Term
52
51
38
36
19
17
15
15
15
13
12
10
8
8
7
5
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
487
219
199
19
19
16
13
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
352
136
8
1
1
Clonality
Polyclonal
Conjugate
This CHEK2 antibody is un-conjugated
10
9
9
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
Application
Western Blotting (WB)
399
126
124
107
90
66
48
43
27
6
6
5
2
1
1
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Catalog No. ABIN2779326
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.905818 ABIN3043811 IHC (p) WB Rabbit IgG AA 465-498, C-Term Log in to see Polyclonal
2.905818 ABIN616051 WB Rabbit AA 514-518 Log in to see Polyclonal 2
2.905818 ABIN968596 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
2.905818 ABIN968597 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
2.905818 ABIN487312 IHC (p) IP WB Mouse IgG2a Log in to see DCS-270 4
2.905818 ABIN129576 ELISA WB Rabbit IgG AA 64-73, pThr68 Log in to see Polyclonal 1
2.905818 ABIN151943 ICC IF WB Mouse IgG Log in to see 8F12 2
2.905818 ABIN408359 IHC (p) IHC WB Rabbit pThr68 Log in to see Polyclonal 7
2.905818 ABIN393759 IF WB Mouse IgG2a kappa AA 1-544 Log in to see 4B7 5
2.905818 ABIN1881196 FACS IF IHC (p) WB Rabbit AA 111-141, N-Term Log in to see Polyclonal 3
2.905818 ABIN1724714 ICC IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 3
2.905818 ABIN3044245 WB Rabbit IgG AA 133-148, N-Term Log in to see Polyclonal
2.905818 ABIN3043703 WB Rabbit IgG AA 427-441, C-Term Log in to see Polyclonal
2.905818 ABIN1531802 ELISA WB Rabbit IgG pSer516 Log in to see Polyclonal
2.905818 ABIN1531803 IHC ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
2.905818 ABIN1531263 IHC ELISA WB Rabbit IgG pThr387 Log in to see Polyclonal
2.905818 ABIN1531498 IF ELISA WB Rabbit IgG pThr383 Log in to see Polyclonal
2.905818 ABIN2855002 WB Rabbit IgG C-Term Log in to see Polyclonal
2.905818 ABIN3182426 ELISA IF WB Rabbit IgG pThr383 Log in to see Polyclonal
2.905818 ABIN3181963 ELISA WB Rabbit IgG pSer516 Log in to see Polyclonal

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope N-Term
(52), (51), (38), (36), (19), (17), (15), (15), (15), (13), (12), (10), (8), (8), (7), (5), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(487), (219), (199), (19), (19), (16), (13), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(352), (136), (8), (1), (1)
Clonality
Polyclonal
Conjugate This CHEK2 antibody is un-conjugated
(10), (9), (9), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Application Western Blotting (WB)
(399), (126), (124), (107), (90), (66), (48), (43), (27), (6), (6), (5), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-CHEK2 Antibody

Target Details CHEK2 Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN2779326) Images
Blocking Peptide Blocking peptide for this product available: ABIN974906
Sequence SRESDVEAQQ SHGSSACSQP HGSVTQSQGS SSQSQGISSS STSTMPNSSQ
Predicted Reactivity Human: 100%
Characteristics This is a rabbit polyclonal antibody against CHEK2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2

Target Details CHEK2

Product Details anti-CHEK2 Antibody Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN2779326) Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Alias Symbols: CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1
Protein Interaction Partner: SRPK1, PPP2CA, PLK1, CHEK2, PRKDC, ATM, POLR2L, RCHY1, VHL, UBC, NR4A1, ELAVL1, CDC25A, ARHGAP1, PER3, CDC25C, CUL1, CUL4A, KPNA2, APP, STRAP, TP53, BRCA1, RB1, DAPK3, UBR5, PML, MDM2, BRCA2, KAT2B, LATS2, PSME3, TRIM28, RNF8, STAT1, PPP2R5E, PPP2R5D, PPP
Protein Size: 586
Molecular Weight 65 kDa
Gene ID 11200
NCBI Accession NM_001005735, NP_001005735
UniProt B7ZBF3
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product Details anti-CHEK2 Antibody Target Details CHEK2 Handling ProductDetails: References for anti-CHEK2 antibody (ABIN2779326) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 586 AA

Restrictions For Research Use only

Handling

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details ProductDetails: References for anti-CHEK2 antibody (ABIN2779326) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-CHEK2 antibody (ABIN2779326)

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling Images back to top
Background publications

Li, Baskaran, Krisky, Bein, Grandi, Cohen, Glorioso: "Chk2 is required for HSV-1 ICP0-mediated G2/M arrest and enhancement of virus growth." in: Virology, Vol. 375, Issue 1, pp. 13-23, 2008

Images

Product Details anti-CHEK2 Antibody Target Details CHEK2 Application Details Handling ProductDetails: References for anti-CHEK2 antibody (ABIN2779326) back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN2779326) Host: Rabbit
Target Name: CHEK2
Sample Tissue: Human Adult Placenta
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN2779326) Host: Rabbit
Target Name: CHEK2
Sample Tissue: Human Fetal Muscle