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anti-Human Checkpoint Kinase 2 antibody for Western Blotting

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Epitope
N-Term
44
42
39
32
24
15
13
13
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
454
186
167
10
9
8
8
7
5
1
1
1
Host
Rabbit
332
128
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Western Blotting (WB)
372
127
124
82
75
56
45
40
25
6
5
2
1
1
Supplier
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Catalog No. ABIN2779326
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13433187 ABIN616051 WB Rabbit AA 514-518 Log in to see Polyclonal 2
0.13433187 ABIN2779312 IHC WB Rabbit N-Term Log in to see Polyclonal 3
0.13433187 ABIN487312 IHC (p) IP WB Mouse IgG2a Log in to see DCS-270 4
0.13433187 ABIN499619 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
0.13433187 ABIN117975 EIA WB Rabbit AA 64-73, pThr68 Log in to see Polyclonal 3
0.13433187 ABIN968596 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
0.13433187 ABIN968597 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
0.13433187 ABIN129576 ELISA WB Rabbit IgG AA 64-73, pThr68 Log in to see Polyclonal 1
0.13433187 ABIN393759 IF WB Mouse IgG2a kappa AA 1-544 Log in to see 4B7 5
0.13433187 ABIN1724714 ICC IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 3
0.13433187 ABIN1881196 FACS IF IHC (p) WB Rabbit AA 111-141, N-Term Log in to see Polyclonal 3
0.13433187 ABIN1531498 IF ELISA WB Rabbit IgG pThr383 Log in to see Polyclonal
0.13433187 ABIN1531802 ELISA WB Rabbit IgG pSer516 Log in to see Polyclonal
0.13433187 ABIN1531803 IHC ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
0.13433187 ABIN1531263 IHC ELISA WB Rabbit IgG pThr387 Log in to see Polyclonal
0.13433187 ABIN487488 IHC (p) IP WB Mouse IgG2a Log in to see DCS-273
0.13433187 ABIN1106683 WB Rabbit IgG C-Term Log in to see Polyclonal
0.13433187 ABIN1532281 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
0.13433187 ABIN1532512 ELISA WB Rabbit IgG Log in to see Polyclonal
0.13433187 ABIN1532833 IHC ELISA WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Checkpoint Kinase 2 antibody for Western Blotting

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human
ELISA 113 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 25 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 46 Antibodies
Immunofluorescence (IF) 83 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 56 Antibodies
Immunohistochemistry (IHC) 127 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 75 Antibodies
Immunoprecipitation (IP) 39 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 362 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope N-Term
(44), (42), (39), (32), (24), (15), (13), (13), (12), (11), (10), (9), (9), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(454), (186), (167), (10), (9), (8), (8), (7), (5), (1), (1), (1)
Host Rabbit
(332), (128), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Western Blotting (WB)
(372), (127), (124), (82), (75), (56), (45), (40), (25), (6), (5), (2), (1), (1)
Pubmed 1 reference available
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Product details

Target details Application Details Handling References Images
Blocking Peptide Blocking peptide for this product available: ABIN974906
Sequence SRESDVEAQQ SHGSSACSQP HGSVTQSQGS SSQSQGISSS STSTMPNSSQ
Predicted Reactivity Human : 100 %
Characteristics This is a rabbit polyclonal antibody against CHEK2. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human CHEK2

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHEK2 (CHEK2 Antibody Abstract)
Background In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. CHEK2 is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by this gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
Molecular Weight 65 kDa
Gene ID 11200
NCBI Accession NM_001005735, NP_001005735
UniProt B7ZBF3
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling References Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 586 AA

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References

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Background publications

Li, Baskaran, Krisky, Bein, Grandi, Cohen, Glorioso: "Chk2 is required for HSV-1 ICP0-mediated G2/M arrest and enhancement of virus growth." in: Virology, Vol. 375, Issue 1, pp. 13-23, 2008

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN2779326) Host: Rabbit
Target Name: CHEK2
Sample Tissue: Human Adult Placenta
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (N-Term) antibody (ABIN2779326) Host: Rabbit
Target Name: CHEK2
Sample Tissue: Human Fetal Muscle