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anti-Human Checkpoint Kinase 2 antibody for Western Blotting

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CHK-2
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • MCYG_07308
  • LOC396525
  • CHEK2
  • Chek2
  • chek2
Epitope
AA 514-518
48
47
38
34
20
17
15
15
13
12
12
10
10
8
6
6
5
5
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
474
227
208
19
19
16
14
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
345
131
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
8
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Western Blotting (WB)
388
131
128
99
82
66
46
41
26
6
6
5
2
1
1
Supplier
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Catalog No. ABIN616051
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9590726 ABIN2779326 WB Rabbit N-Term Log in to see Polyclonal 1
2.9590726 ABIN487312 IHC (p) IP WB Mouse IgG2a Log in to see DCS-270 4
2.9590726 ABIN2779312 IHC WB Rabbit N-Term Log in to see Polyclonal 3
2.9590726 ABIN499619 EIA IF WB Rabbit IgG N-Term Log in to see Polyclonal 4
2.9590726 ABIN117975 EIA WB Rabbit AA 64-73, pThr68 Log in to see Polyclonal 3
2.9590726 ABIN129576 ELISA WB Rabbit IgG AA 64-73, pThr68 Log in to see Polyclonal 1
2.9590726 ABIN968597 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
2.9590726 ABIN968596 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
2.9590726 ABIN151943 ICC IF WB Mouse IgG Log in to see 8F12 2
2.9590726 ABIN408359 IHC (p) IHC WB Rabbit pThr68 Log in to see Polyclonal 7
2.9590726 ABIN393759 IF WB Mouse IgG2a kappa AA 1-544 Log in to see 4B7 5
2.9590726 ABIN1724714 ICC IHC ELISA WB Mouse IgG2b AA 481-531 Log in to see 1C12B8 3
2.9590726 ABIN1881196 FACS IF IHC (p) WB Rabbit AA 111-141, N-Term Log in to see Polyclonal 3
2.9590726 ABIN1531263 IHC ELISA WB Rabbit IgG pThr387 Log in to see Polyclonal
2.9590726 ABIN1531498 IF ELISA WB Rabbit IgG pThr383 Log in to see Polyclonal
2.9590726 ABIN1531802 ELISA WB Rabbit IgG pSer516 Log in to see Polyclonal
2.9590726 ABIN1531803 IHC ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
2.9590726 ABIN2855002 WB Rabbit IgG C-Term Log in to see Polyclonal
2.9590726 ABIN3043811 IHC (p) WB Rabbit IgG AA 465-498, C-Term Log in to see Polyclonal
2.9590726 ABIN3183905 ELISA WB Rabbit IgG Tyr594, Tyr604 Log in to see Polyclonal

Top referenced anti-Checkpoint Kinase 2 antibody for Western Blotting

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human
ELISA 119 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies
Flow Cytometry (FACS) 26 Antibodies
Immunochromatography (IC) 6 Antibodies
Immunocytochemistry (ICC) 41 Antibodies
Immunofluorescence (IF) 82 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 6 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 66 Antibodies
Immunohistochemistry (IHC) 130 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 98 Antibodies
Immunoprecipitation (IP) 45 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 380 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 514-518
(48), (47), (38), (34), (20), (17), (15), (15), (13), (12), (12), (10), (10), (8), (6), (6), (5), (5), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(474), (227), (208), (19), (19), (16), (14), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(345), (131), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(9), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Western Blotting (WB)
(388), (131), (128), (99), (82), (66), (46), (41), (26), (6), (6), (5), (2), (1), (1)
Pubmed 2 references available
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Product details

Target details Application Details Handling References Images
Sequence Q-P-S-T-S
Specificity This antibody detects endogenous levels of total Chk2 protein.
Cross-Reactivity (Details) Species reactivity (tested):Human
Purification Affinity-chromatography using epitope-specific peptide
Immunogen Peptide sequence around aa 514~518 derived from Chk2

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Molecular Weight 62 kDa
Gene ID 11200
NCBI Accession NP_001005735
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling References Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1.0 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.

References

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Background publications

Melchionna, Chen, Blasina, McGowan: "Threonine 68 is required for radiation-induced phosphorylation and activation of Cds1." in: Nature cell biology, Vol. 2, Issue 10, pp. 762-5, 2000

Ahn, Schwarz, Piwnica-Worms, Canman: "Threonine 68 phosphorylation by ataxia telangiectasia mutated is required for efficient activation of Chk2 in response to ionizing radiation." in: Cancer research, Vol. 60, Issue 21, pp. 5934-6, 2000

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 514-518) antibody (ABIN616051) Western blot analysis of extract from HL60 cells using Chk2.