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anti-Monkey Checkpoint Kinase 2 antibody for Enzyme Immunoassay

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • Cds1
  • CDS1
  • CHK-2
  • Chk2
  • CHK2
  • fa66f08
  • hCds1
  • HuCds1
  • HUCDS1
  • LFS2
  • PP1425
  • Rad53
  • RAD53
  • wu:fa66f08
  • zgc:55865
Epitope
AA 64-73, pThr68
44
40
39
32
24
15
13
13
12
11
10
9
8
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
408
160
159
10
8
7
7
6
5
1
1
1
Host
Rabbit
323
91
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
7
7
5
5
5
5
5
5
5
5
5
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
326
120
117
64
63
56
37
26
24
6
4
2
1
Supplier
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Catalog No. ABIN318831
$ 544.50
Plus shipping costs $45.00

Top referenced anti-Checkpoint Kinase 2 antibody for Enzyme Immunoassay

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus) Monkey
Western Blotting (WB) 316 Antibodies 109 Antibodies 109 Antibodies 7 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Luminex Assay (LMNX) 2 Antibodies
Immunoprecipitation (IP) 36 Antibodies 6 Antibodies 5 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 64 Antibodies 25 Antibodies 24 Antibodies
Immunohistochemistry (IHC) 117 Antibodies 49 Antibodies 48 Antibodies 3 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 64-73, pThr68
(44), (40), (39), (32), (24), (15), (13), (13), (12), (11), (10), (9), (8), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(408), (160), (159), (10), (8), (7), (7), (6), (5), (1), (1), (1)
Host Rabbit
(323), (91), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (2), (2), (2), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(326), (120), (117), (64), (63), (56), (37), (26), (24), (6), (4), (2), (1)
Pubmed 1 reference available
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Product details

Target details Application Details Handling References Images
Specificity This antibody detects Checkpoint kinase 2 (Chk2), phosphorylated at Threonine 68 (Thr68).
Cross-Reactivity (Details) Species reactivity (expected):Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.
Purification Purified
Immunogen Synthetic peptide corresponding to amino acids 64-73 of human Chk2.
Isotype IgG

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Chk2 is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radiation, and phsophorylate Thr68 and other sites in the amino-terminal region of Chk2, leading to Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, preventing the entry into mitosis, and has been shown to stabilize the tumour suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumour suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familial cancer usually associated with inherited mutations in TP53.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2
Gene ID 11200
NCBI Accession NP_001005735
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling References Images back to top
Application Notes ELISA: 1: 10000 - 1: 50000. Western Blot: 1: 200 - 1: 2000, detects a band of approximately 61 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Concentration 1.0mg/mL
Buffer PBS, pH 7.2, 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References

Product details Target details Application Details Handling Images back to top
Background publications

Bartek, Lukas: "Chk1 and Chk2 kinases in checkpoint control and cancer." in: Cancer cell, Vol. 3, Issue 5, pp. 421-9, 2003