|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Monkey|
|Western Blotting (WB)||316 Antibodies||109 Antibodies||109 Antibodies||7 Antibodies|
|Proximity Ligation Assay (PLA)||1 Antibodies|
|Luminex Assay (LMNX)||2 Antibodies|
|Immunoprecipitation (IP)||36 Antibodies||6 Antibodies||5 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||64 Antibodies||25 Antibodies||24 Antibodies|
|Immunohistochemistry (IHC)||117 Antibodies||49 Antibodies||48 Antibodies||3 Antibodies|
|Antigen||Checkpoint Kinase 2 (CHEK2) Antibodies|
|Epitope||AA 64-73, pThr68 Alternatives|
|Reactivity||Human, Monkey, Mouse (Murine), Rat (Rattus) Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|1 reference available|
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Product detailsTarget details Application Details Handling References Images
|Specificity||This antibody detects Checkpoint kinase 2 (Chk2), phosphorylated at Threonine 68 (Thr68).|
Species reactivity (expected):Chimpanzee, Mouse, Rat.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to amino acids 64-73 of human Chk2.|
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|Alternative Name||CHK2 (CHEK2 Antibody Abstract)|
|Background||Chk2 is a serine/threonine-protein kinase which functions as a cell cycle checkpoint regulator. ATM and ATR kinases are activated in response to ionizing and ultraviolet radiation, and phsophorylate Thr68 and other sites in the amino-terminal region of Chk2, leading to Chk2 dimerisation and activation. Activated Chk2 phosphorylates and inhibits Cdc25C phosphatase, preventing the entry into mitosis, and has been shown to stabilize the tumour suppressor protein p53, leading to cell cycle arrest in G1. In addition, Chk2 can phosphorylate BRCA1, allowing BRCA1 to restore survival after DNA damage. Chk2 is a putative tumour suppressor protein, with mutations in to the Chk2 gene linked to Li-Fraumeni syndrome, a familial cancer usually associated with inherited mutations in TP53.Synonyms: CHEK-2, CHEK2, CHK-2, CHK2 checkpoint homolog, Cds1, RAD53, Serine/threonine-protein kinase Chk2|
|Pathways||p53 Signaling, Apoptosis, Cell Division Cycle|
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ELISA: 1: 10000 - 1: 50000. Western Blot: 1: 200 - 1: 2000, detects a band of approximately 61 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
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|Buffer||PBS, pH 7.2, 0.01 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
ReferencesProduct details Target details Application Details Handling Images back to top
Bartek, Lukas: "Chk1 and Chk2 kinases in checkpoint control and cancer." in: Cancer cell, Vol. 3, Issue 5, pp. 421-9, 2003