anti-Mouse (Murine) Checkpoint Kinase 2 antibody for Western Blotting

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CHK-2
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • MCYG_07308
  • LOC396525
  • CHEK2
  • Chek2
  • chek2
Epitope
pThr68
48
48
37
34
19
17
15
15
14
13
12
10
8
7
6
5
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
459
216
197
19
19
16
14
8
5
4
2
1
1
1
1
1
1
Host
Rabbit
330
131
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
9
8
8
7
7
5
5
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
Application
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
375
119
116
98
83
66
44
42
26
6
6
5
2
1
1
Supplier
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Catalog No. ABIN408359
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.1955817 ABIN968596 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
3.1955817 ABIN968597 IF WB Mouse IgG1 AA 31-234 Log in to see 19-Chk2 2
3.1955817 ABIN3043811 IHC (p) WB Rabbit IgG AA 465-498, C-Term Log in to see Polyclonal
3.1955817 ABIN1531263 IHC ELISA WB Rabbit IgG pThr387 Log in to see Polyclonal
3.1955817 ABIN1531498 IF ELISA WB Rabbit IgG pThr383 Log in to see Polyclonal
3.1955817 ABIN1531803 IHC ELISA WB Rabbit IgG pThr68 Log in to see Polyclonal
3.1955817 ABIN3182510 ELISA IHC WB Rabbit IgG pThr387 Log in to see Polyclonal
3.1955817 ABIN3183906 ELISA IHC WB Rabbit IgG Tyr599 Log in to see Polyclonal
3.1955817 ABIN3183904 ELISA IHC WB Rabbit IgG Tyr594 Log in to see Polyclonal
3.1955817 ABIN3183907 ELISA IHC WB Rabbit IgG Tyr60 Log in to see Polyclonal
3.1955817 ABIN3182426 ELISA IF WB Rabbit IgG pThr383 Log in to see Polyclonal
3.1955817 ABIN3181964 ELISA IHC WB Rabbit IgG pThr68 Log in to see Polyclonal
3.1955817 ABIN1106683 WB Rabbit IgG C-Term Log in to see Polyclonal
3.1955817 ABIN1532512 ELISA WB Rabbit IgG Log in to see Polyclonal
3.1955817 ABIN1532281 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1955817 ABIN1532833 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
3.1955817 ABIN2705870 IHC WB Rabbit pSer387 Log in to see Polyclonal
3.1955817 ABIN2705871 IC IF WB Rabbit pSer383 Log in to see Polyclonal
3.1955817 ABIN223048 IF WB Rabbit IgG N-Term Log in to see Polyclonal
3.1955817 ABIN1871829 ICC IF IHC IP WB Rabbit IgG Log in to see Polyclonal

Top referenced anti-Checkpoint Kinase 2 antibody for Western Blotting

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 43 Antibodies 43 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 16 Antibodies 16 Antibodies
Immunofluorescence (IF) 27 Antibodies 26 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 4 Antibodies 4 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 52 Antibodies
Immunohistochemistry (IHC) 56 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 37 Antibodies
Immunoprecipitation (IP) 9 Antibodies
Western Blotting (WB) 134 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope pThr68
(48), (48), (37), (34), (19), (17), (15), (15), (14), (13), (12), (10), (8), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(459), (216), (197), (19), (19), (16), (14), (8), (5), (4), (2), (1), (1), (1), (1), (1), (1)
Host Rabbit
(330), (131), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(9), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1)
Application Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
(375), (119), (116), (98), (83), (66), (44), (42), (26), (6), (6), (5), (2), (1), (1)
Pubmed 7 references available
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Product details

Target details Application Details Handling References Images
Specificity Chk2 [p Thr68] Antibody detects endogenous levels of Chk2 protein only when phosphorylated at T68.
Purification Immunogen affinity purified
Immunogen Synthetic phosphopeptide derived from human Chk2 around the phosphorylation site of Threonine 68.

Target details

Product details Application Details Handling References Images back to top
Antigen
Alternative Name CHK2 (CHEK2 Antibody Abstract)
Background Gene Symbol: CHEK2
Molecular Weight Theoretical MW: 62 kDa
Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

Product details Target details Handling References Images back to top
Application Notes Western Blot 1:500-1:1000, Immunohistochemistry, Immunohistochemistry-Paraffin 1:50-1:200Western blot (WB) analysis of p-Chk2 [p Thr68] pAb in extracts from NIH/3T3 cells treated with UV. Immunohistochemistry (IHC) analyzes of p-Chk2 [p Thr68] pAb in paraffin-embedded human breast carcinoma tissue. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product details Target details Application Details References Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer PBS ( pH 7.2)
Buffer contains: 0.05 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References

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Product cited in:

Ercilla, Llopis, Feu, Aranda, Ernfors, Freire, Agell: "New origin firing is inhibited by APC/CCdh1 activation in S-phase after severe replication stress." in: Nucleic acids research, Vol. 44, Issue 10, pp. 4745-62, 2016 Method employed by authors: Immunohistochemistry (IHC)

Domínguez, Feijoo, Bernal, Ercilla, Agell, Genescà, Tusell: "Centrosome aberrations in human mammary epithelial cells driven by cooperative interactions between p16INK4a deficiency and telomere-dependent genotoxic stress." in: Oncotarget, Vol. 6, Issue 29, pp. 28238-56, 2015 (Sample species: Human). Further details: Western Blotting

Rao, Tortola, Perlot, Wirnsberger, Novatchkova, Nitsch, Sykacek, Frank, Schramek, Komnenovic, Sigl, Aumayr, Schmauss, Fellner, Handschuh, Glösmann, Pasierbek, Schlederer, Resch, Ma, Yang, Popper et al.: "A dual role for autophagy in a murine model of lung cancer. ..." in: Nature communications, Vol. 5, pp. 3056, 2014 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Ma, Wei, Dong, Huo, Dong: "DNA damage response in renal ischemia-reperfusion and ATP-depletion injury of renal tubular cells." in: Biochimica et biophysica acta, Vol. 1842, Issue 7, pp. 1088-96, 2014 Further details: Western Blotting

Velimezi, Liontos, Vougas, Roumeliotis, Bartkova, Sideridou, Dereli-Oz, Kocylowski, Pateras, Evangelou, Kotsinas, Orsolic, Bursac, Cokaric-Brdovcak, Zoumpourlis, Kletsas, Papafotiou, Klinakis et al.: "Functional interplay between the DNA-damage-response kinase ATM and ARF tumour suppressor protein in human cancer. ..." in: Nature cell biology, Vol. 15, Issue 8, pp. 967-77, 2013 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Evangelou, Bartkova, Kotsinas, Pateras, Liontos, Velimezi, Kosar, Liloglou, Trougakos, Dyrskjot, Andersen, Papaioannou, Drosos, Papafotiou, Hodny, Sosa-Pineda, Wu, Klinakis, Ørntoft, Lukas, Bartek et al.: "The DNA damage checkpoint precedes activation of ARF in response to escalating oncogenic stress during tumorigenesis. ..." in: Cell death and differentiation, Vol. 20, Issue 11, pp. 1485-97, 2013 (Sample species: Human). Further details: Western Blotting,Immunohistochemistry (Paraffin-embedded Sections)

Schramek, Kotsinas, Meixner, Wada, Elling, Pospisilik, Neely, Zwick, Sigl, Forni, Serrano, Gorgoulis, Penninger: "The stress kinase MKK7 couples oncogenic stress to p53 stability and tumor suppression." in: Nature genetics, Vol. 43, Issue 3, pp. 212-9, 2011 (Sample species: Mouse (Murine)). Further details: Immunohistochemistry (Paraffin-embedded Sections)

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Immunohistochemistry-Paraffin: p-Chk2 [p Thr68] Antibody [ABIN4083592] - Paraffin-emb...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Immunohistochemistry-Paraffin: p-Chk2 [p Thr68] Antibody [ABIN4083592] - Human breast...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Western Blot: p-Chk2 [p Thr68] Antibody [ABIN4083592] - (WB) analysis of p-Chk2 [p Th...
Western Blotting (WB) image for anti-Checkpoint Kinase 2 (CHEK2) (pThr68) antibody (ABIN408359) Western Blot: p-Chk2 [p Thr68] Antibody [ABIN4083592] - Lane1:MCF-7 whole cell lysate...