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anti-Rat (Rattus) Checkpoint Kinase 2 antibody for Immunofluorescence (Paraffin-embedded Sections)

Recommended Checkpoint Kinase 2 Antibody (supplied by: Log in to see )

Antigen
Checkpoint Kinase 2 (CHEK2) Antibodies
  • CHK-2
  • CDS1
  • CHK2
  • HuCds1
  • LFS2
  • PP1425
  • RAD53
  • hCds1
  • Cds1
  • HUCDS1
  • Rad53
  • Chk2
  • fa66f08
  • wu:fa66f08
  • zgc:55865
  • serine/threonine-protein kinase chk2
  • blue sensitive cone opsin
  • checkpoint kinase 2
  • CHK2 checkpoint homolog (S. pombe)
  • MCYG_07308
  • LOC396525
  • CHEK2
  • Chek2
  • chek2
Epitope
AA 50-90, pThr68
45
45
38
32
22
15
13
12
12
11
10
9
9
7
6
6
5
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
461
189
171
10
10
9
8
7
3
1
1
1
Host
Rabbit
337
128
8
1
Clonality
Polyclonal
Conjugate
Un-conjugated
8
8
8
7
7
5
5
5
5
5
5
5
4
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
376
133
129
90
84
55
46
40
26
6
5
2
1
1
Options
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Catalog No. ABIN703165
$ 291.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN685867 IF (p) IHC (p) WB Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN878463 IF (p) Alexa Fluor 488 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN878464 IF (p) Alexa Fluor 555 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN878462 IF (p) Alexa Fluor 350 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN878465 IF (p) Alexa Fluor 647 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703168 IF (p) Cy3 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703170 IF (p) Cy5.5 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703172 IF (p) FITC Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703169 IF (p) Cy5 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN703171 IF (p) Cy7 Rabbit IgG AA 50-90, pThr68 Log in to see Polyclonal
1 ABIN685871 IF (p) Cy5 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685872 IF (p) Cy5.5 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685874 IF (p) FITC Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685873 IF (p) Cy7 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN685870 IF (p) Cy3 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN890470 IF (p) Alexa Fluor 488 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN890468 IF (p) Alexa Fluor 555 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN890469 IF (p) Alexa Fluor 350 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN890471 IF (p) Alexa Fluor 647 Rabbit IgG AA 120-160 Log in to see Polyclonal
1 ABIN2811610 IF (p) Alexa Fluor 594 Rabbit IgG AA 120-160 Log in to see Polyclonal

Top referenced anti-Checkpoint Kinase 2 antibody for Immunofluorescence (Paraffin-embedded Sections)

Similar anti-Checkpoint Kinase 2 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
ELISA 47 Antibodies 47 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies 2 Antibodies
Flow Cytometry (FACS) 2 Antibodies 1 Antibodies
Immunochromatography (IC) 1 Antibodies 1 Antibodies
Immunocytochemistry (ICC) 15 Antibodies 15 Antibodies
Immunofluorescence (IF) 25 Antibodies 24 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 44 Antibodies 44 Antibodies
Immunohistochemistry (IHC) 55 Antibodies 58 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 5 Antibodies
Western Blotting (WB) 119 Antibodies

General

Antigen Checkpoint Kinase 2 (CHEK2) Antibodies
Epitope AA 50-90, pThr68
(45), (45), (38), (32), (22), (15), (13), (12), (12), (11), (10), (9), (9), (7), (6), (6), (5), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(461), (189), (171), (10), (10), (9), (8), (7), (3), (1), (1), (1)
Host Rabbit
(337), (128), (8), (1)
Clonality
Polyclonal
Conjugate Un-conjugated
(8), (8), (8), (7), (7), (5), (5), (5), (5), (5), (5), (5), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(376), (133), (129), (90), (84), (55), (46), (40), (26), (6), (5), (2), (1), (1)
Pubmed 1 reference available
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Product details

Target details Application Details Handling References Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic phosphopeptide derived from human CHK2 around the phosphorylation site of Thr68
Isotype IgG

Target details

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Antigen
Alternative Name Chk2 (CHEK2 Antibody Abstract)
Background

Serine/threonine-protein kinase which is required for checkpoint-mediated cell cycle arrest, activation of DNA repair and apoptosis in response to the presence of DNA double-strand breaks. May also negatively regulate cell cycle progression during unperturbed cell cycles. Following activation, phosphorylates numerous effectors preferentially at the consensus sequence [L-X-R-X-X-S/T]. Regulates cell cycle checkpoint arrest through phosphorylation of CDC25A, CDC25B and CDC25C, inhibiting their activity. Inhibition of CDC25 phosphatase activity leads to increased inhibitory tyrosine phosphorylation of CDK-cyclin complexes and blocks cell cycle progression. May also phosphorylate NEK6 which is involved in G2/M cell cycle arrest. Regulates DNA repair through phosphorylation of BRCA2, enhancing the association of RAD51 with chromatin which promotes DNA repair by homologous recombination. Also stimulates the transcription of genes involved in DNA repair (including BRCA2) through the phosphorylation and activation of the transcription factor FOXM1. Regulates apoptosis through the phosphorylation of p53/TP53, MDM4 and PML. Phosphorylation of p53/TP53 at 'Ser-20' by CHEK2 may alleviate inhibition by MDM2, leading to accumulation of active p53/TP53. Phosphorylation of MDM4 may also reduce degradation of p53/TP53. Also controls the transcription of pro-apoptotic genes through phosphorylation of the transcription factor E2F1. Tumor suppressor, it may also have a DNA damage-independent function in mitotic spindle assembly by phosphorylating BRCA1. Its absence may be a cause of the chromosomal instability observed in some cancer cells.

Subcellular location: Nucleus


Synonyms: CDS1,CHK2,LFS2,RAD53,hCds1,HuCds1,PP1425,Serine/threonine-protein kinase Chk2,CHK2 checkpoint homolog,Cds1 homolog,Checkpoint kinase 2,CHEK2
Gene ID 11200
UniProt O96017
Pathways p53 Signaling, Apoptosis, Cell Division Cycle

Application Details

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Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

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Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References

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Product cited in:

Yin, Jiang, Peng, Cui, Zhou, He, Zuo, Ouyang, Fan, Fang: "The molecular mechanism of G2M cell cycle arrest induced by AFB1 in the jejunum." in: Oncotarget, Vol. 7, Issue 24, pp. 35592-35606, 2016

Images

Product details Target details Application Details Handling References back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 50-90), (pThr68) antibody (ABIN703165) Formalin-fixed and paraffin embedded human cervical carcinoma labeled with Anti-Phosp...
SDS-PAGE (SDS) image for anti-Checkpoint Kinase 2 (CHEK2) (AA 50-90), (pThr68) antibody (ABIN703165) L1 rat brain lysates, L2 human colon carcinoma lysates probed with Anti- Phospho-CHK2...