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anti-Human E2F1 antibody for Enzyme Immunoassay

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
pSer337
31
27
27
25
25
15
13
13
10
10
9
9
9
8
8
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
350
72
29
9
4
3
3
3
1
1
1
Host
Rabbit
273
66
19
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
273
159
76
54
53
50
41
36
23
11
11
2
2
1
1
1
Supplier
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Catalog No. ABIN358119
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008854274 ABIN358416 EIA Rabbit Ig pSer332 Log in to see Polyclonal 5
0.0008854274 ABIN359768 EIA WB Rabbit Ig Ser332 Log in to see Polyclonal 5
0.0008854274 ABIN359769 EIA WB Rabbit Ig His357 Log in to see Polyclonal 5
0.0008854274 ABIN401277 EIA IHC (p) WB Rabbit IgG AA 360-369, pSer364 Log in to see Polyclonal 5
0.0008854274 ABIN492942 EIA IP WB Rabbit AA 138-144 Log in to see Polyclonal 4
0.0008854274 ABIN492940 EIA IP WB Rabbit AA 58-83 Log in to see Polyclonal 4
0.0008854274 ABIN2712090 EIA IP WB Rabbit AA 392-414, C-Term Log in to see Polyclonal 4
0.0008854274 ABIN303220 EIA IHC (p) WB Rabbit AA 360-369, pSer364 Log in to see Polyclonal
0.0008854274 ABIN1107042 EIA IHC (fro) WB Rabbit acLys120, acLys125 Log in to see Polyclonal
0.0008854274 ABIN1107043 EIA IF WB Rabbit acLys120, acLys125 Log in to see Polyclonal
0.0008854274 ABIN2719893 EIA ICC IF IHC (p) Rabbit IgG Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Enzyme Immunoassay

Similar anti-E2F1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 41 Antibodies
ELISA 153 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 11 Antibodies
Gel Shift (GS) 2 Antibodies
Immunocytochemistry (ICC) 27 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 56 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 282 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope pSer337
(31), (27), (27), (25), (25), (15), (13), (13), (10), (10), (9), (9), (9), (8), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(350), (72), (29), (9), (4), (3), (3), (3), (1), (1), (1)
Host Rabbit
(273), (66), (19), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(273), (159), (76), (54), (53), (50), (41), (36), (23), (11), (11), (2), (2), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN358119) Images
Specificity This antibody detects E2F1 pSer337.
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S337 of human E2F1.
Isotype Ig

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN358119) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Molecular Weight 46920 Da
Gene ID 1869, 9606
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN358119) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN358119) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-E2F1 Antibody (ABIN358119)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

ODonnell, Wentzel, Zeller, Dang, Mendell: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005

Joshi, Ordonez-Ercan, Dasgupta, Chellappan: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005

Saberwal, Lucas, Janssen, Deobhakta, Hu, Galili, Raza, Mundle: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004

Images

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Supplier Images
 image for anti-E2F1 antibody (E2F Transcription Factor 1) (pSer337) (ABIN358119) anti-E2F Transcription Factor 1 (E2F1) (pSer337) antibody
 image for anti-E2F1 antibody (E2F Transcription Factor 1) (pSer337) (ABIN358119) anti-E2F Transcription Factor 1 (E2F1) (pSer337) antibody (Image 2)