anti-Human E2F1 antibody for Immunocytochemistry

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Reactivity
Human
425
125
47
10
8
6
6
5
5
4
3
3
2
1
1
Host
Mouse
288
125
21
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
25
23
20
15
13
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
337
181
110
85
79
57
55
42
39
28
9
3
2
2
1
1
1
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Catalog No. ABIN153153
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 16G7 1
1 ABIN2854981 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN441399 ICC IF WB Rabbit IgG Center Log in to see Polyclonal
1 ABIN153152 ICC IF IP WB Mouse IgG2b Log in to see 10D1 2B5
1 ABIN932458 DB ICC Mouse IgG2b Log in to see 658C2a
1 ABIN2856879 ICC IF WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN1831921 DB ICC Mouse IgG2b Log in to see 658C2a
1 ABIN5076415 ICC IF WB Rabbit IgG Log in to see Polyclonal
1 ABIN5626568 ICC IF WB DyLight 350 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626574 ICC IF WB DyLight 755 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626578 ICC IF IHC IHC (p) WB DyLight 550 Mouse IgG1 AA 244-264 Log in to see 16G7
1 ABIN5626564 ICC IF WB Alexa Fluor 488 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626572 ICC IF WB DyLight 650 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626573 ICC IF WB DyLight 680 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626575 ICC IF FITC Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626577 ICC IF IHC IHC (p) WB Biotin Mouse IgG1 AA 244-264 Log in to see 16G7
1 ABIN5626579 ICC IF IHC IHC (p) WB DyLight 650 Mouse IgG1 AA 244-264 Log in to see 16G7
1 ABIN5626580 ICC IF IHC IHC (p) FITC Mouse IgG1 AA 244-264 Log in to see 16G7
1 ABIN5626565 ICC IF WB Alexa Fluor 647 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5
1 ABIN5626563 ICC IF WB Alexa Fluor 405 Mouse IgG2b AA 223-332 Log in to see 10D1 2B5

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Reactivity Human
(425), (125), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(288), (125), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(25), (23), (20), (15), (13), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (IHC), Western Blotting (WB)
(337), (181), (110), (85), (79), (57), (55), (42), (39), (28), (9), (3), (2), (2), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN153153) Images
Purification Protein G purified
Immunogen The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
Clone 18E10
Isotype IgG1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 antibody (ABIN153153) Images back to top
Antigen
Alternative Name E2F-1 (E2F1 Antibody Abstract)
Background Gene Symbol: E2F1
Molecular Weight Theoretical MW: 47 kDa
Gene ID 1869
UniProt Q01094
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 antibody (ABIN153153) Images back to top
Application Notes Western Blot, Immunohistochemistry 1:10 - 1:500, Immunocytochemistry/Immunofluorescence, ImmunoprecipitationThe observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 antibody (ABIN153153) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer PBS ( pH 7.4)
Buffer contains: No Preservative
Preservative Without preservative
Handling Advice Avoid freeze-thaw cycles
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

References for anti-E2F1 antibody (ABIN153153)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Malhotra, Lapointe, Salari, Higgins, Ferrari, Montgomery, van de Rijn, Brooks, Pollack: "A tri-marker proliferation index predicts biochemical recurrence after surgery for prostate cancer." in: PLoS ONE, Vol. 6, Issue 5, pp. e20293, 2011 (Sample species: Human). Further details: Immunohistochemistry

Liu, Clements, Zhao, Marmorstein: "Structure of the human Papillomavirus E7 oncoprotein and its mechanism for inactivation of the retinoblastoma tumor suppressor." in: The Journal of biological chemistry, Vol. 281, Issue 1, pp. 578-86, 2006 (Sample species: Human). Further details: Immunohistochemistry

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 antibody (ABIN153153) back to top
Supplier Images
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) antibody (ABIN153153) Western Blot: E2F1 Antibody (18E10) [ABIN153153] - Sample (30 ug of whole cell lysate...