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anti-Human E2F1 antibody for Immunofluorescence

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
Center
31
28
27
25
25
15
13
13
10
10
9
9
9
8
7
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
334
56
28
9
4
3
3
3
1
1
1
Host
Rabbit
271
52
19
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
13
13
13
3
3
3
3
3
3
3
3
3
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
257
158
60
41
40
38
36
35
12
10
8
2
2
1
1
1
Supplier
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Catalog No. ABIN2854981
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.29896915 ABIN783034 IF WB Rabbit IgG AA 133-322 Log in to see Polyclonal
0.29896915 ABIN2157244 IF WB Rabbit IgG Log in to see Polyclonal
0.29896915 ABIN2856879 ICC IF WB Rabbit Internal Region Log in to see Polyclonal
0.0011172801 ABIN115204 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95 1
0.0011172801 ABIN115203 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95
0.0011172801 ABIN386825 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa Log in to see KH95
0.0011172801 ABIN1107043 EIA IF WB Rabbit acLys120, acLys125 Log in to see Polyclonal
0.0011172801 ABIN200168 IF IP WB Mouse IgG1 AA 244-264 Log in to see 16G7
0.0011172801 ABIN200169 IF IP WB Mouse IgG1 AA 244-264 Log in to see 18E10
0.0011172801 ABIN386827 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa Log in to see KH95
0.0011172801 ABIN1886014 IF WB Rabbit AA 133-322 Log in to see Polyclonal
0.0011172801 ABIN2256897 IF IP IHC WB Mouse IgG2a kappa AA 342-386 Log in to see 3F375
0.0011172801 ABIN441399 ICC IF WB Rabbit IgG Log in to see Polyclonal
0.0011172801 ABIN2256853 FACS IF ELISA WB Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0011172801 ABIN2256887 FACS IF ELISA WB FITC Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0011172801 ABIN2256889 FACS IF ELISA WB PE Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0011172801 ABIN2256885 FACS IF ELISA WB APC Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0011172801 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa AA 244-264 Log in to see 16G7
0.0011172801 ABIN153152 ICC IF IP WB Mouse IgG2b AA 223-332 Log in to see 2B5
0.0011172801 ABIN386828 IF IHC (p) IP WB Mouse IgG2a, kappa Log in to see KH95

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
ELISA 22 Antibodies 152 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 2 Antibodies 10 Antibodies
Immunocytochemistry (ICC) 2 Antibodies 13 Antibodies
Immunofluorescence (IF) 5 Antibodies 32 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 7 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 55 Antibodies
Dot Blot (DB) 41 Antibodies
Gel Shift (GS) 2 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope Center
(31), (28), (27), (25), (25), (15), (13), (13), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(334), (56), (28), (9), (4), (3), (3), (3), (1), (1), (1)
Host Rabbit
(271), (52), (19), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (13), (13), (13), (3), (3), (3), (3), (3), (3), (3), (3), (3)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(257), (158), (60), (41), (40), (38), (36), (35), (12), (10), (8), (2), (2), (1), (1), (1)
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Cross-Reactivity Chicken, Rat (Rattus), Cow (Bovine)
Cross-Reactivity (Details) Chicken (86 %), Rat (97 %), Bovine (96 %)
Characteristics Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)
E2F1 antibody [N1N3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Cellular Localization: Nucleus
Molecular Weight 47 kDa
Gene ID 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) WB Image Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane w...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted ...
Immunoprecipitation (IP) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) IP Image E2F1 antibody [N1N3] immunoprecipitates E2F1 protein in IP experiments. IP s...
Immunofluorescence (IF) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) ICC/IF Image E2F1 antibody [N1N3] detects E2F1 protein at nucleus by immunofluorescen...