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anti-Human E2F1 antibody for Immunofluorescence

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
AA 133-322
32
28
27
25
25
14
14
13
12
10
10
9
9
8
8
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
363
79
36
9
4
3
3
3
1
1
1
Host
Rabbit
279
73
19
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunofluorescence (IF), Western Blotting (WB)
286
169
80
60
54
53
41
36
26
12
12
2
2
2
1
1
Supplier
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Catalog No. ABIN783034
$ 401.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.24556193 ABIN2854981 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal
0.24556193 ABIN441399 ICC IF WB Rabbit IgG Log in to see Polyclonal
0.24556193 ABIN4306768 ICC IF IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
0.24556193 ABIN2856879 ICC IF WB Rabbit Internal Region Log in to see Polyclonal
0.24556193 ABIN2157244 IF WB Rabbit IgG Log in to see Polyclonal
0.24556193 ABIN153152 ICC IF IP WB Mouse IgG2b kappa Log in to see 2B5
0.0010825092 ABIN115204 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95 1
0.0010825092 ABIN115203 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95
0.0010825092 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 16G7 1
0.0010825092 ABIN153153 ICC IF IP IHC WB Mouse IgG1 kappa Log in to see 1-80E11 2
0.0010825092 ABIN386825 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa Log in to see KH95
0.0010825092 ABIN386827 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa Log in to see KH95
0.0010825092 ABIN1107043 EIA IF WB Rabbit acLys120, acLys125 Log in to see Polyclonal
0.0010825092 ABIN2256897 IF IP IHC WB Mouse IgG2a kappa AA 342-386 Log in to see 3F375
0.0010825092 ABIN1886014 IF WB Rabbit AA 133-322 Log in to see Polyclonal
0.0010825092 ABIN386828 IF IHC (p) IP WB Mouse IgG2a, kappa Log in to see KH95
0.0010825092 ABIN2256853 FACS IF ELISA WB Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010825092 ABIN2256889 FACS IF ELISA WB PE Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010825092 ABIN2256885 FACS IF ELISA WB APC Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal
0.0010825092 ABIN2256887 FACS IF ELISA WB FITC Rabbit IgG AA 109-138, Center, Internal Region Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Immunofluorescence

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Application / Reactivity Human
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ELISA 161 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Gel Shift (GS) 2 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 54 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 287 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 133-322
(32), (28), (27), (25), (25), (14), (14), (13), (12), (10), (10), (9), (9), (8), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(363), (79), (36), (9), (4), (3), (3), (3), (1), (1), (1)
Host Rabbit
(279), (73), (19), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunofluorescence (IF), Western Blotting (WB)
(286), (169), (80), (60), (54), (53), (41), (36), (26), (12), (12), (2), (2), (2), (1), (1)
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Specificity Recognizes E2F transcription factor 1.
Cellular Localization: Nucleus.
Cross-Reactivity (Details) Species reactivity (expected):Mouse (85 %), Rat (81 %).
Species reactivity (tested):Human.
Purification Antigen-Affinity Chromatography.
Immunogen Recombinant protein fragment contain a sequence corresponding to a region within amino acids 133 and 322 of E2F1
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Gene ID 1869, 9606
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.1 M Tris, 0.1 M Glycine, 10 % Glycerol and 0.01 % Thimerosal as preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody undiluted (in aliquots) at -20 °C.

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 133-322) (ABIN783034) Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using AP23781PU-N E2F1 an...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 133-322) (ABIN783034) Western Blot using AP23781PU-N E2F1 antibody at 1/1000 dilution. Sample (30 µg of who...