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anti-Human E2F1 antibody for Immunoprecipitation

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
Center
30
30
30
27
25
15
14
14
12
11
10
9
9
8
8
8
6
6
6
4
4
4
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
365
97
44
9
8
6
6
5
5
4
3
3
2
1
1
Host
Rabbit
280
73
20
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
284
162
78
59
58
53
42
39
24
13
12
3
2
2
1
1
1
Options
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Catalog No. ABIN2854981
$ 358.60
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN115204 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95 1
1 ABIN492942 EIA IP WB Rabbit AA 138-144 Log in to see Polyclonal 4
1 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 16G7 1
1 ABIN153153 ICC IF IP IHC WB Mouse IgG1 Log in to see 18E10 2
1 ABIN115203 IF IHC (p) IP WB Mouse IgG2a Log in to see KH95
1 ABIN2712090 EIA IP WB Rabbit AA 392-414, C-Term Log in to see Polyclonal 4
1 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5
1 ABIN492940 EIA IP WB Rabbit AA 58-83 Log in to see Polyclonal 4
1 ABIN386823 IF IHC (p) IP WB Mouse IgG2a, kappa AA 342-386 Log in to see KH95
1 ABIN210874 IP WB Mouse IgG AA 1-89, AA 342-386 Log in to see KH20-KH95
1 ABIN4903534 IF IP WB Mouse IgG Log in to see
1 ABIN153150 IP WB Mouse IgG1 Log in to see 17E2
1 ABIN153151 IP WB Mouse IgG1 Log in to see 6B5
1 ABIN153152 ICC IF IP WB Mouse IgG2b Log in to see 10D1 2B5
1 ABIN333021 IP WB Rabbit IgG AA 310-329 Log in to see Polyclonal
1 ABIN386827 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa AA 342-386 Log in to see KH95
1 ABIN386825 GS IF IHC (p) IP WB Biotin Mouse IgG2a, kappa AA 342-386 Log in to see KH95
1 ABIN189992 IP WB Mouse IgG1 AA 332-390 Log in to see
1 ABIN189994 IP WB Mouse IgG1 AA 244-264 Log in to see
1 ABIN189993 IP WB Mouse IgG1 AA 223-322 Log in to see

Top referenced anti-E2F1 antibody for Immunoprecipitation

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
ELISA 33 Antibodies 154 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 5 Antibodies 13 Antibodies
Gel Shift (GS) 3 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 93 Antibodies
Dot Blot (DB) 39 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope Center
(30), (30), (30), (27), (25), (15), (14), (14), (12), (11), (10), (9), (9), (8), (8), (8), (6), (6), (6), (4), (4), (4), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(365), (97), (44), (9), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Rabbit
(280), (73), (20), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
(284), (162), (78), (59), (58), (53), (42), (39), (24), (13), (12), (3), (2), (2), (1), (1), (1)
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling Images
Cross-Reactivity Chicken, Rat (Rattus), Cow (Bovine)
Cross-Reactivity (Details) Chicken (86 %), Rat (97 %), Bovine (96 %)
Characteristics Rabbit Polyclonal antibody to E2F1 (E2F transcription factor 1)
E2F1 antibody [N1N3]
Purification Purified by antigen-affinity chromatography.
Immunogen Recombinant protein encompassing a sequence within the center region of human E2F1. The exact sequence is proprietary.
Isotype IgG

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Cellular Localization: Nucleus
Molecular Weight 47 kDa
Gene ID 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling Images back to top
Application Notes Suggested dilution Reference ICC/IF 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*
Comment

Positive Control: 293T , A431 , H1299 , HeLaS3 , HepG2 , Molt-4 , Raji , NIH-3T3

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.
Preservative Thimerosal (Merthiolate)
Precaution of Use This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) WB Image Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane w...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted ...
Immunoprecipitation (IP) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) IP Image E2F1 antibody [N1N3] immunoprecipitates E2F1 protein in IP experiments. IP s...
Immunofluorescence (IF) image for anti-E2F1 antibody (E2F Transcription Factor 1) (Center) (ABIN2854981) ICC/IF Image E2F1 antibody [N1N3] detects E2F1 protein at nucleus by immunofluorescen...