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anti-Human E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • ATE2FB
  • CG6376
  • dE2f
  • dE2F
  • dE2F1
  • dE2f1
  • de2f1
  • def21
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • drosE2F1
  • DRTF1/E2F
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • e2f
  • E2F
  • E2F-1
  • e2f1
  • E2f1
  • E2F1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • Evar(3)164
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • mKIAA4009
  • RBAP1
  • RBBP3
  • RBP3
  • xE2F
Epitope
His357
32
28
27
25
25
14
13
13
12
10
10
9
9
8
8
7
7
6
5
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
363
79
36
9
4
3
3
3
1
1
1
Host
Rabbit
279
73
19
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
19
18
16
14
13
13
4
4
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
286
169
80
60
54
54
41
36
26
12
11
2
2
2
1
1
Supplier
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Catalog No. ABIN359769
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.1205588 ABIN359768 EIA WB Rabbit Ig Ser332 Log in to see Polyclonal 5
0.1205588 ABIN2792180 WB Rabbit C-Term Log in to see Polyclonal 1
0.1205588 ABIN2792603 WB Rabbit N-Term Log in to see Polyclonal 1
0.1205588 ABIN391857 WB Rabbit Ig AA 310-344, Ser332 Log in to see Polyclonal 5
0.1205588 ABIN391858 WB Rabbit Ig AA 342-371, His357 Log in to see Polyclonal 6
0.1205588 ABIN389553 IHC (p) WB Rabbit Ig pSer337 Log in to see Polyclonal 5
0.1205588 ABIN969516 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see 8G9 3
0.1205588 ABIN1449255 WB Mouse IgG2b pSer364 Log in to see #2 3
0.1205588 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5
0.1205588 ABIN1531270 ELISA WB Rabbit IgG pThr433 Log in to see Polyclonal
0.1205588 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 kappa Log in to see 16G7 1
0.1205588 ABIN153153 ICC IF IP IHC WB Mouse IgG1 kappa Log in to see 1-80E11 2
0.1205588 ABIN783034 IF WB Rabbit IgG AA 133-322 Log in to see Polyclonal
0.1205588 ABIN1532290 ELISA WB Rabbit IgG Log in to see Polyclonal
0.1205588 ABIN2854981 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal
0.1205588 ABIN3044440 WB Rabbit IgG AA 246-260, Middle Region Log in to see Polyclonal
0.1205588 ABIN2704741 IHC WB Rabbit C-Term Log in to see Polyclonal
0.1205588 ABIN2704740 WB Rabbit C-Term, pSer433 Log in to see Polyclonal
0.1205588 ABIN2777217 WB Rabbit Middle Region Log in to see Polyclonal
0.1205588 ABIN2460125 ELISA WB Rabbit Log in to see Polyclonal

Top referenced anti-E2F1 antibody for Western Blotting

Similar anti-E2F1 Antibodies

Application / Reactivity Human
Dot Blot (DB) 41 Antibodies
ELISA 161 Antibodies
Enzyme Immunoassay (EIA) 12 Antibodies
Flow Cytometry (FACS) 12 Antibodies
Gel Shift (GS) 2 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 36 Antibodies
Immunohistochemistry (IHC) 80 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 54 Antibodies
Immunoprecipitation (IP) 60 Antibodies
Proximity Ligation Assay (PLA) 2 Antibodies
Western Blotting (WB) 287 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope His357
(32), (28), (27), (25), (25), (14), (13), (13), (12), (10), (10), (9), (9), (8), (8), (7), (7), (6), (5), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(363), (79), (36), (9), (4), (3), (3), (3), (1), (1), (1)
Host Rabbit
(279), (73), (19), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(19), (18), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Western Blotting (WB)
(286), (169), (80), (60), (54), (54), (41), (36), (26), (12), (11), (2), (2), (2), (1), (1)
Pubmed 5 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN359769) Images
Specificity This antibody reacts to E2F1.
Purification Protein A column, followed by peptide affinity purification
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding H357 of human E2F1.
Isotype Ig

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN359769) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another two members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.Synonyms: E2F transcription factor 1, E2F-1, PBR3, PRB-binding protein E2F-1, RBBP3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1
Gene ID 1869, 9606
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN359769) Images back to top
Application Notes ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN359769) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

References for anti-E2F1 Antibody (ABIN359769)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

ODonnell, Wentzel, Zeller, Dang, Mendell: "c-Myc-regulated microRNAs modulate E2F1 expression." in: Nature, Vol. 435, Issue 7043, pp. 839-43, 2005

Wang, Hou, Mohapatra, Ma, Cress, Pledger, Chen: "Activation of p27Kip1 Expression by E2F1. A negative feedback mechanism." in: The Journal of biological chemistry, Vol. 280, Issue 13, pp. 12339-43, 2005

Joshi, Ordonez-Ercan, Dasgupta, Chellappan: "Induction of human metallothionein 1G promoter by VEGF and heavy metals: differential involvement of E2F and metal transcription factors." in: Oncogene, Vol. 24, Issue 13, pp. 2204-17, 2005

Saberwal, Lucas, Janssen, Deobhakta, Hu, Galili, Raza, Mundle: "Increased levels and activity of E2F1 transcription factor in myelodysplastic bone marrow." in: International journal of hematology, Vol. 80, Issue 2, pp. 146-54, 2004

Chaussepied, Ginsberg: "Transcriptional regulation of AKT activation by E2F." in: Molecular cell, Vol. 16, Issue 5, pp. 831-7, 2004

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN359769) back to top
Supplier Images
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (His357) (ABIN359769) Western blot analysis of anti-E2F1 Antibody in 293 cell line lysates (35ug/lane). E2F...