anti-Human E2F1 antibody for Western Blotting

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
N-Term
44
36
31
28
22
21
17
15
14
14
12
11
11
11
10
9
9
8
8
7
6
5
5
4
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
425
124
46
10
8
5
5
4
4
4
3
3
2
1
1
Host
Rabbit
287
126
21
1
Clonality
Polyclonal
Conjugate
This E2F1 antibody is un-conjugated
25
23
20
15
13
13
8
7
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
3
2
2
Application
Western Blotting (WB)
337
181
111
86
79
58
56
42
39
28
9
3
2
2
1
1
1
Options
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Catalog No. ABIN2792603
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
2.9058237 ABIN2792180 WB Rabbit C-Term Log in to see Polyclonal 1
2.9058237 ABIN359768 EIA WB Rabbit Ig Ser332 Log in to see Polyclonal 5
2.9058237 ABIN359769 EIA WB Rabbit Ig His357 Log in to see Polyclonal 5
2.9058237 ABIN389553 IHC (p) WB Rabbit Ig pSer337 Log in to see Polyclonal 5
2.9058237 ABIN391858 WB Rabbit Ig AA 342-371, His357 Log in to see Polyclonal 6
2.9058237 ABIN391857 WB Rabbit Ig AA 310-344, Ser332 Log in to see Polyclonal 5
2.9058237 ABIN269710 ICC IF IHC (p) IP IHC WB Mouse IgG1 Log in to see 16G7 1
2.9058237 ABIN153153 ICC IF IP IHC WB Mouse IgG1 Log in to see 18E10 2
2.9058237 ABIN1449255 WB Mouse IgG2b pSer364 Log in to see #2 3
2.9058237 ABIN969516 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see 8G9 3
2.9058237 ABIN3044440 WB Rabbit IgG AA 246-260, Middle Region Log in to see Polyclonal 3
2.9058237 ABIN4306768 IHC IHC (p) WB Rabbit IgG Log in to see Polyclonal 1
2.9058237 ABIN1531270 ELISA WB Rabbit IgG pThr433 Log in to see Polyclonal
2.9058237 ABIN967439 IP WB Mouse IgG2a Log in to see KH95-E2F 5
2.9058237 ABIN2854981 ICC IF IP WB Rabbit IgG Center Log in to see Polyclonal
2.9058237 ABIN3184378 ELISA IHC WB Rabbit IgG Ser310 Log in to see Polyclonal
2.9058237 ABIN2777217 WB Rabbit Middle Region Log in to see Polyclonal
2.9058237 ABIN1532290 ELISA WB Rabbit IgG Log in to see Polyclonal
2.9058237 ABIN153150 IP WB Mouse IgG1 Log in to see 17E2
2.9058237 ABIN2704740 WB Rabbit C-Term, pSer433 Log in to see Polyclonal

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope N-Term
(44), (36), (31), (28), (22), (21), (17), (15), (14), (14), (12), (11), (11), (11), (10), (9), (9), (8), (8), (7), (6), (5), (5), (4), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Dog (Canine), Guinea Pig, Horse (Equine), Human, Mouse (Murine), Rabbit, Rat (Rattus)
(425), (124), (46), (10), (8), (5), (5), (4), (4), (4), (3), (3), (2), (1), (1)
Host Rabbit
(287), (126), (21), (1)
Clonality
Polyclonal
Conjugate This E2F1 antibody is un-conjugated
(25), (23), (20), (15), (13), (13), (8), (7), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2)
Application Western Blotting (WB)
(337), (181), (111), (86), (79), (58), (56), (42), (39), (28), (9), (3), (2), (2), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN2792603) Images
Sequence PARGRGRHPG KGVKSPGEKS RYETSLNLTT KRFLELLSHS ADGVVDLNWA
Cross-Reactivity Cow (Bovine), Dog (Canine), Chicken, Human, Mouse (Murine), Rat (Rattus)
Predicted Reactivity Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%
Characteristics This is a rabbit polyclonal antibody against E2F1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human E2F1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN2792603) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by E2F1 is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: E2F-1, RBBP3, RBP3, RBAP1
Protein Interaction Partner: RB1, SIRT1, KDM2A, LAMTOR5, EP300, KAT2B, VHL, RARA, STAT1, NCOR2, STOML1, RBL1, MAGEA11, SKP2, UHRF2, UBC, GTF2H1, ERCC3, SPT15, TFB1, TBP, NCOA3, CEBPE, PARP1, PRMT2, RBL2, CCNE1, TFDP2, TFDP1, CDKN2A, CEBPD, CDK2, CDK1, NEDD8, MCPH1, DDB2, DDB1, XIAP,
Protein Size: 437
Molecular Weight 47 kDa
Gene ID 1869
NCBI Accession NM_005225, NP_005216
UniProt Q01094
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling ProductDetails: References for anti-E2F1 antibody (ABIN2792603) Images back to top
Application Notes WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:1562500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 437 AA

Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details ProductDetails: References for anti-E2F1 antibody (ABIN2792603) Images back to top
Format Liquid
Concentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freeze-thaw cycles.
Storage -20 °C
Storage Comment For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

ProductDetails: References for anti-E2F1 antibody (ABIN2792603)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Background publications

Hu, Sun, Nadiminty, Lou, Pinder, Gao: "Transcriptional regulation of human RANK ligand gene expression by E2F1." in: Biochemical and biophysical research communications, Vol. 370, Issue 3, pp. 440-4, 2008

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN2792603) back to top
Supplier Images
Western Blotting (WB) image for anti-E2F Transcription Factor 1 (E2F1) (N-Term) antibody (ABIN2792603) WB Suggested Anti-E2F1 Antibody Titration: 0.2-1 ug/ml
ELISA Titer: ...