anti-Mouse (Murine) E2F1 antibody for ELISA

Recommended E2F1 Antibody (supplied by: Log in to see )

Antigen
E2F Transcription Factor 1 (E2F1) Antibodies
  • CG6376
  • DRTF1/E2F
  • DmE2F-1
  • Dmel\\CG6376
  • Dp
  • E(Sev-CycE)3A
  • E(var)3-93E
  • E(var)3-95E
  • E(var)93E
  • E2F
  • E2F-1
  • E2F1
  • E2f1
  • Evar(3)164
  • dE2F
  • dE2F1
  • dE2f
  • dE2f1
  • de2f1
  • def21
  • drosE2F1
  • e2f
  • e2f1
  • l(3)07172
  • l(3)j3B1
  • l(3)j3C2
  • l(3)rM729
  • RBAP1
  • RBBP3
  • RBP3
  • mKIAA4009
  • xE2F
  • ATE2FB
  • E2F TRANSCRIPTION FACTOR-1 E2F1
  • E2F transcription factor 1
  • E2FB
  • E2FB TRANSCRIPTION FACTOR
  • E2F transcription factor
  • E2F transcription factor 1
  • E2f
  • E2F1
  • E2f1
  • e2f1
Epitope
AA 69-223
31
30
30
27
25
15
14
14
12
11
11
10
9
9
8
8
7
6
6
5
5
5
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
371
101
47
10
8
6
6
5
5
4
3
3
2
1
1
Host
Mouse
287
72
21
1
Clonality
Monoclonal
Conjugate
This E2F1 antibody is un-conjugated
21
19
16
14
13
13
4
4
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
290
165
77
60
58
55
42
39
25
12
12
3
2
2
1
1
1
Options
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Catalog No. ABIN969516
$ 430.10
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
9.76768 ABIN4306767 CyTOF ELISA FACS IHC IHC (p) WB Mouse IgG1 Log in to see 8G9
9.76768 ABIN1845545 FACS IHC ELISA WB Mouse IgG1 AA 69-223 Log in to see
1 ABIN1531270 ELISA WB Rabbit IgG pThr433 Log in to see Polyclonal
1 ABIN3184378 ELISA IHC WB Rabbit IgG Ser310 Log in to see Polyclonal
1 ABIN1491636 ELISA FACS IHC (p) WB Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN1532290 ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN2460125 ELISA WB Rabbit Log in to see Polyclonal
1 ABIN2888627 ELISA WB Rabbit IgG AA 388-437 Log in to see Polyclonal
1 ABIN4880694 ELISA FACS IHC WB Mouse IgG1 AA 69-223 Log in to see 8G9
1 ABIN3182197 ELISA WB Rabbit IgG pThr433 Log in to see Polyclonal
1 ABIN408534 IHC (p) IHC ELISA WB Rabbit pThr433 Log in to see Polyclonal
1 ABIN2883720 ELISA WB Rabbit IgG pThr433 Log in to see Polyclonal
1 ABIN571306 IHC ELISA WB Rabbit IgG Internal Region, Lys120 Log in to see Polyclonal
1 ABIN1585777 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
1 ABIN4901417 ELISA WB Rabbit IgG 2meLys80 Log in to see Polyclonal
1 ABIN4901415 ELISA WB Rabbit IgG acLys521 Log in to see Polyclonal
1 ABIN4901416 ELISA WB Rabbit IgG 3meLys5 Log in to see Polyclonal
1 ABIN1085505 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN1085506 IHC ELISA WB Rabbit IgG Log in to see Polyclonal
1 ABIN4901413 ELISA WB Rabbit acLys120 Log in to see Polyclonal

Top referenced anti-E2F1 antibody for ELISA

Similar anti-E2F1 Antibodies

Application / Reactivity Mouse (Murine) Human
Cytometry by Time of Flight (CyTOF) 1 Antibodies 1 Antibodies
ELISA 36 Antibodies 157 Antibodies
Enzyme Immunoassay (EIA) 1 Antibodies 12 Antibodies
Flow Cytometry (FACS) 5 Antibodies 13 Antibodies
Gel Shift (GS) 3 Antibodies
Immunocytochemistry (ICC) 16 Antibodies
Immunofluorescence (IF) 22 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 3 Antibodies
Immunohistochemistry (IHC) 37 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 1 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 29 Antibodies
Immunoprecipitation (IP) 22 Antibodies
Proximity Ligation Assay (PLA) 1 Antibodies
Western Blotting (WB) 97 Antibodies
Dot Blot (DB) 39 Antibodies

General

Antigen E2F Transcription Factor 1 (E2F1) Antibodies
Epitope AA 69-223
(31), (30), (30), (27), (25), (15), (14), (14), (12), (11), (11), (10), (9), (9), (8), (8), (7), (6), (6), (5), (5), (5), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine)
(371), (101), (47), (10), (8), (6), (6), (5), (5), (4), (3), (3), (2), (1), (1)
Host Mouse
(287), (72), (21), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This E2F1 antibody is un-conjugated
(21), (19), (16), (14), (13), (13), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(290), (165), (77), (60), (58), (55), (42), (39), (25), (12), (12), (3), (2), (2), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-E2F1 Antibody

Target Details E2F1 Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN969516) Images
Purification Purified
Immunogen Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. Coli.
Clone 8G9
Isotype IgG1

Target Details E2F1

Product Details anti-E2F1 Antibody Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN969516) Images back to top
Antigen
Alternative Name E2F1 (E2F1 Antibody Abstract)
Background The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
Molecular Weight 46.9 kDa
Gene ID 1869
HGNC 1869
Research Area Cancer, Transcription Factors
Pathways p53 Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy

Application Details

Product Details anti-E2F1 Antibody Target Details E2F1 Handling ProductDetails: References for anti-E2F1 antibody (ABIN969516) Images back to top
Application Notes ELISA: 1:10000, WB: 1:500 - 1:2000, IHC: 1:200 - 1:1000, FCM: 1:200 - 1:400
Restrictions For Research Use only

Handling

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details ProductDetails: References for anti-E2F1 antibody (ABIN969516) Images back to top
Format Liquid
Buffer PBS with 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment 4°C, -20°C for long term storage

ProductDetails: References for anti-E2F1 antibody (ABIN969516)

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
Product cited in:

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, 2010

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, 2010

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, 2010

Images

Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling ProductDetails: References for anti-E2F1 antibody (ABIN969516) back to top
Supplier Images
ELISA image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 1: Red: Control Antigen (100 ng), Purple: Antigen (10ng), Green: Antigen (50 n...
Flow Cytometry (FACS) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 2: Flow cytometric analysis of HeLa cells using E2F1 mouse mAb (green) and neg...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 3: Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues...
Immunohistochemistry (IHC) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 4: Immunohistochemical analysis of paraffin-embedded rectum cancer tissues usi...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 5: Western blot analysis using E2F1 mouse mAb against HeLa (1), SK-N-SH (2), a...
Western Blotting (WB) image for anti-E2F1 antibody (E2F Transcription Factor 1) (AA 69-223) (ABIN969516) Figure 6: Western blot analysis using E2F1 mAb against human E2F1 recombinant protein...