We are preparing the requested document. Please wait, this may take a while...!
|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||22 Antibodies||152 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||12 Antibodies|
|Flow Cytometry (FACS)||2 Antibodies||10 Antibodies|
|Immunocytochemistry (ICC)||2 Antibodies||13 Antibodies|
|Immunofluorescence (IF)||5 Antibodies||32 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||6 Antibodies|
|Immunohistochemistry (IHC)||17 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||1 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||7 Antibodies|
|Immunoprecipitation (IP)||4 Antibodies|
|Western Blotting (WB)||55 Antibodies|
|Dot Blot (DB)||41 Antibodies|
|Gel Shift (GS)||2 Antibodies|
|Immunofluorescence (Cultured Cells) (IF (cc))||1 Antibodies|
|Antigen||E2F Transcription Factor 1 (E2F1) Antibodies|
|Epitope||AA 69-223 Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This E2F1 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
|3 references available|
|Supplier||Log in to see|
Product Details anti-E2F1 AntibodyTarget Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images
|Immunogen||Purified recombinant fragment of human E2F1 (AA 69-223) expressed in E. coli.|
Target Details E2F1Product Details anti-E2F1 Antibody Application Details Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
|Alternative Name||E2F1 (E2F1 Antibody Abstract)|
|Background||The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.|
|Molecular Weight||46.9 kDa|
|Research Area||Cancer, Transcription Factors|
|Pathways||p53 Signaling, Cell Division Cycle|
Application DetailsProduct Details anti-E2F1 Antibody Target Details E2F1 Handling References for anti-E2F1 Antibody (ABIN969516) Images back to top
ELISA: 1/10000, WB: 1/500 - 1/2000, IHC: 1/200 - 1/1000, FCM: 1/200 - 1/400
Not yet tested in other applications.
Determining optimal working dilutions by titration test.
|Restrictions||For Research Use only|
HandlingProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details References for anti-E2F1 Antibody (ABIN969516) Images back to top
|Buffer||PBS with 0.05 % sodium azide and 0.5 % protein stabilizer|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store at 4 °C or at -20 °C for long term.|
References for anti-E2F1 Antibody (ABIN969516)Product Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling Images back to top
|Product cited in:||
Gurtner, Fuschi, Martelli et al.: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." in: Cancer research, Vol. 70, Issue 23, pp. 9711-20, 2010 (PubMed).
Okazaki, Matsunaga, Okazaki et al.: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." in: Cancer research, Vol. 70, Issue 15, pp. 6238-46, 2010 (PubMed).
Locatelli, Bosotti, Ciomei et al.: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." in: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, 2010 (PubMed).
ImagesProduct Details anti-E2F1 Antibody Target Details E2F1 Application Details Handling References for anti-E2F1 Antibody (ABIN969516) back to top