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anti-Human FAS antibody for Flow Cytometry

Recommended FAS Antibody (supplied by: Log in to see )

Antigen
Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
  • AI196731
  • APO1
  • APT1
  • CD95
  • TNFR6
  • Tnfrsf6
  • lpr
  • TNFRSF6
  • ALPS1A
  • APO-1
  • FAS1
  • FASTM
  • A630082H08Rik
  • FAS
  • Fas (TNF receptor superfamily member 6)
  • Fas (TNF receptor superfamily, member 6)
  • Fas cell surface death receptor
  • fatty acid synthase
  • Fas
  • FAS
  • Fasn
Reactivity
Human
664
108
97
15
10
6
4
3
3
3
3
2
1
Host
Mouse
421
301
18
5
4
3
3
Clonality
Monoclonal
Conjugate
This FAS antibody is un-conjugated
77
64
52
31
16
8
6
6
4
4
4
4
4
4
4
4
4
3
2
2
2
2
2
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
331
313
213
147
116
83
60
52
52
46
21
19
12
7
7
5
5
4
4
3
3
3
2
2
2
1
1
1
1
1
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Catalog No. ABIN238438
$ 253.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.149551 ABIN1106623 FACS PE Mouse IgG2a Log in to see B-G27 1
13.149551 ABIN492367 FACS APC Mouse IgG1 Log in to see LT95 6
13.149551 ABIN1106613 FACS Neut WB Mouse IgG1 Log in to see ZB4 11
13.149551 ABIN1106620 IHC (fro) FACS Mouse IgG2a Log in to see B-G27 3
13.149551 ABIN1106622 FACS FITC Mouse IgG2a Log in to see B-G27 3
13.149551 ABIN1106617 EIA FACS WB Mouse IgG1 Log in to see 4F8D6 2
13.149551 ABIN1106616 EIA FACS IF WB Mouse IgG1 Log in to see 4F8H6 2
13.149551 ABIN1177357 Func FACS Mouse IgG1, kappa Log in to see DX2 2
13.149551 ABIN969518 ICC FACS ELISA WB Mouse IgG1 Log in to see 4F8H6 2
13.149551 ABIN969519 FACS ELISA WB Mouse IgG1 Log in to see 4F8D6 2
13.149551 ABIN94250 Func FACS Mouse IgM Log in to see UT-1 4
13.149551 ABIN371582 FACS FITC Mouse IgG2a Log in to see DX3 1
13.149551 ABIN2661155 FACS Biotin Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2657878 BR FACS IP IHC Alexa Fluor 647 Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2659571 FACS PE-Cy7 Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2658744 FACS ICFC APC Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2660170 FACS PerCP-Cy5.5 Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2657495 FACS ICFC Alexa Fluor 488 Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2662323 FACS IF IHC Pacific Blue Mouse IgG1 kappa Log in to see DX2 8
13.149551 ABIN2661868 FACS FITC Mouse IgG1 kappa Log in to see DX2 8

Top referenced anti-FAS antibody for Flow Cytometry

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Application / Reactivity Human
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Biochemical Assay (BCA) 1 Antibodies
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Blocking Reagent (BR) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 19 Antibodies
ELISA 187 Antibodies
ELISA (Capture) 1 Antibodies
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Immunofluorescence (IF) 46 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 5 Antibodies

General

Antigen Fas (TNF Receptor Superfamily, Member 6) (FAS) Antibodies
Reactivity Human
(664), (108), (97), (15), (10), (6), (4), (3), (3), (3), (3), (2), (1)
Host Mouse
(421), (301), (18), (5), (4), (3), (3)
Clonality (Clone)
Monoclonal   ( )
Conjugate This FAS antibody is un-conjugated
(77), (64), (52), (31), (16), (8), (6), (6), (4), (4), (4), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
(331), (313), (213), (147), (116), (83), (60), (52), (52), (46), (21), (19), (12), (7), (7), (5), (5), (4), (4), (3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1)
Pubmed 6 references available
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Product Details anti-FAS Antibody

Target Details FAS Application Details Handling References for anti-FAS Antibody (ABIN238438) Images
Specificity The antibody LT95 reacts with CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Cross-Reactivity (Details) Species reactivity (tested):Human.
Characteristics Synonyms: FASLG receptor, Apo-1 antigen, APT1, FAS1, TNFRSF6, Tumor necrosis factor receptorsuperfamily member 6
Purification Precipitation methods and Ion exchange chromatography.
Immunogen HUT-78 human T cell lymphoma cell line
Clone LT95
Isotype IgG1

Target Details FAS

Product Details anti-FAS Antibody Application Details Handling References for anti-FAS Antibody (ABIN238438) Images back to top
Antigen
Alternative Name CD95 / FAS (FAS Antibody Abstract)
Background CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.Synonyms: APT1, Apo-1 antigen, FAS1, FASLG receptor, TNFRSF6, Tumor necrosis factor receptor superfamily member 6
Gene ID 355
UniProt P25445
Pathways p53 Signaling, Apoptosis, TCR Signaling

Application Details

Product Details anti-FAS Antibody Target Details FAS Handling References for anti-FAS Antibody (ABIN238438) Images back to top
Application Notes Flow Cytometry. Immunohistochemistry (paraffin sections). Positive tissue: tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-FAS Antibody Target Details FAS Application Details References for anti-FAS Antibody (ABIN238438) Images back to top
Concentration 1.0 mg/mL
Buffer Tris buffered saline (TBS), pH 8.0, 15 mM Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.
Expiry Date 12 months

References for anti-FAS Antibody (ABIN238438)

Product Details anti-FAS Antibody Target Details FAS Application Details Handling Images back to top
Background publications

Brumatti, Yon, Castro, Bueno-da-Silva, Jacysyn, Brunner, Amarante-Mendes: "Conversion of CD95 (Fas) Type II into Type I signaling by sub-lethal doses of cycloheximide." in: Experimental cell research, Vol. 314, Issue 3, pp. 554-63, 2008

Guo, Zhang, Tang, Cao: "Fas signal links innate and adaptive immunity by promoting dendritic-cell secretion of CC and CXC chemokines." in: Blood, Vol. 106, Issue 6, pp. 2033-41, 2005

Drosopoulos, Roberts, Cermak, Sasazuki, Shirasawa, Andera, Pintzas: "Transformation by oncogenic RAS sensitizes human colon cells to TRAIL-induced apoptosis by up-regulating death receptor 4 and death receptor 5 through a MEK-dependent pathway." in: The Journal of biological chemistry, Vol. 280, Issue 24, pp. 22856-67, 2005

Nakamura, Kakinuma, Amada, Miyata, Taniguchi, Koda, Sato: "Pyrazole derivatives as new potent and selective 20-hydroxy-5,8,11,14-eicosatetraenoic acid synthase inhibitors." in: Bioorganic & medicinal chemistry, Vol. 12, Issue 23, pp. 6209-19, 2004

Park, Thomsen, Frevert, Pham, Skerrett, Kiener, Liles: "Fas (CD95) induces proinflammatory cytokine responses by human monocytes and monocyte-derived macrophages." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 170, Issue 12, pp. 6209-16, 2003

Scaffidi, Fulda, Srinivasan, Friesen, Li, Tomaselli, Debatin, Krammer, Peter: "Two CD95 (APO-1/Fas) signaling pathways." in: The EMBO journal, Vol. 17, Issue 6, pp. 1675-87, 1998

Images

Product Details anti-FAS Antibody Target Details FAS Application Details Handling References for anti-FAS Antibody (ABIN238438) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-FAS antibody (Fas (TNF Receptor Superfamily, Member 6)) (ABIN238438) anti-Fas (TNF Receptor Superfamily, Member 6) (FAS) antibody